Recombinant Human Bestrophin/BEST1 protein (denatured)

Specifications

Form

Liquid

Additional notes

ab177633 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA

General info

Function

Ligand-gated anion channel that allows the movement of anions across cell membranes when activated by calcium (Ca2+) (PubMed : 11904445, PubMed : 12907679, PubMed : 18179881, PubMed : 18400985, PubMed : 19853238, PubMed : 21330666, PubMed : 26200502, PubMed : 26720466, PubMed : 35789156). Allows the movement of chloride and hydrogencarbonate (PubMed : 11904445, PubMed : 12907679, PubMed : 18179881, PubMed : 18400985, PubMed : 19853238, PubMed : 21330666, PubMed : 26200502, PubMed : 26720466, PubMed : 35789156). Found in a partially open conformation leading to significantly smaller chloride movement (PubMed : 35789156). Upon F2R/PAR-1 activation, the sequestered calcium is released into the cytosol of astrocytes, leading to the (Ca2+)-dependent release of L-glutamate into the synaptic cleft that targets the neuronal postsynaptic GRIN2A/NMDAR receptor resulting in the synaptic plasticity regulation (By similarity). Upon activation of the norepinephrine-alpha-1 adrenergic receptor signaling pathway, transports as well D-serine than L-glutamate in a (Ca2+)-dependent manner, leading to activation of adjacent NMDAR receptors and therefore regulates the heterosynaptic long-term depression and metaplasticity during initial memory acquisition (By similarity). Releases the 4-aminobutanoate neurotransmitter in a (Ca2+)-dependent manner, and participates in its tonic release from cerebellar glial cells (By similarity).

Sequence similarities

Belongs to the anion channel-forming bestrophin (TC 1.A.46) family. Calcium-sensitive chloride channel subfamily.