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AB243124

Recombinant human beta 2 Defensin/BD-2 protein (Active)

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Recombinant human beta 2 Defensin/BD-2 protein (Active) is a Human Full Length protein, in the 24 to 64 aa range, expressed in Escherichia coli, with >98%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS, HPLC.

View Alternative Names

DEFB102, DEFB2, DEFB4, DEFB4B, DEFB4A, Defensin beta 4A, Beta-defensin 2, Skin-antimicrobial peptide 1, BD-2, hBD-2, SAP1

1 Images
SDS-PAGE - Recombinant human beta 2 Defensin/BD-2 protein (Active) (AB243124)
  • SDS-PAGE

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SDS-PAGE - Recombinant human beta 2 Defensin/BD-2 protein (Active) (AB243124)

SDS Page analysis of ab243124

Key facts

Purity

>98% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE, HPLC

applications

Biologically active

Yes

Biological activity

Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using immature human dendritic cells is in a concentration range of 10-100 ng/ml.

Accession

O15263

Animal free

No

Carrier free

Yes

Species

Human

Reconstitution

Reconstitute in water or 0.1% BSA aqueous buffer

Storage buffer

Constituents: 0.75% Sodium chloride, 0.16% Sodium phosphate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "HPLC": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"GIGDPVTCLKSGAICHPVFCPRRYKQIGTCGLPGTKCCKKP","proteinLength":"Full Length","predictedMolecularWeight":"4.3 kDa","actualMolecularWeight":null,"aminoAcidEnd":64,"aminoAcidStart":24,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O15263","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta 2 defensin also known as BD-2 or beta-defensin 2 is a small antimicrobial peptide with a molecular mass of about 4.6 kDa. It plays an important role in the innate immune response by disrupting microbial membranes. BD-2 is expressed in epithelial cells making it important for creating a protective barrier in the skin respiratory tract and urogenital tract. This peptide acts directly against bacteria fungi and some viruses helping to maintain microbial homeostasis in these areas.
Biological function summary

Beta 2 defensin serves as a critical component of host defense mechanisms. It not only shows antimicrobial properties but also influences inflammatory processes. BD-2 interacts with immune cells recruiting and activating them for enhanced defense response. Presently there is no evidence of beta 2 defensin being part of a larger protein complex focusing its function on immediate immune response and cellular signaling in areas it is expressed.

Pathways

Beta 2 defensin engages in critical pathways of immune protection. It is part of the toll-like receptor signaling pathway and engages the NF-kB pathway. These pathways are essential for activating immune responses when pathogens are recognized. Additionally beta 2 defensin interacts with other defensins and related antimicrobial peptides to mount an effective immune response coordinating with various cytokines and chemokines to balance protective actions.

Beta 2 defensin has connections to inflammatory and autoimmune conditions. It has a potential link to psoriasis where its expression levels are increased serving both protective and inflammatory roles. BD-2 is also investigated in cystic fibrosis where its impaired function can contribute to chronic lung infections. In these conditions the altered balance of BD-2 associates it with proteins involved in immune dysregulation contributing to the pathophysiology of these disorders.
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Specifications

Form

Lyophilized

Additional notes

Purity >98% as determined by SDS-PAGE and HPLC.

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General info

Function

Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria, with highest activity against Gram-negative bacteria (PubMed : 10837369, PubMed : 9202117). Antimicrobial activity against P.aruginosa seems to be salt-sensitive and is reduced with high salt concentrations greater than 25 mM (PubMed : 10837369). Also exhibits antimicrobial activity against the yeast C.albicans (PubMed : 10837369, PubMed : 30050988, PubMed : 9202117). Permeabilizes C.albicans cell membranes via targeting plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2), thereby leading to cell fragmentation and cell death (PubMed : 30050988). Acts as a ligand for C-C chemokine receptor CCR6 (PubMed : 10521347, PubMed : 20068036). Binds to CCR6 and induces chemotactic activity of CCR6-expressing cells, such as immature dendritic cells and memory T cells (PubMed : 10521347, PubMed : 20068036).

Sequence similarities

Belongs to the beta-defensin family. LAP/TAP subfamily.

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Product protocols

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Target data

Exhibits antimicrobial activity against Gram-negative bacteria and Gram-positive bacteria, with highest activity against Gram-negative bacteria (PubMed : 10837369, PubMed : 9202117). Antimicrobial activity against P.aruginosa seems to be salt-sensitive and is reduced with high salt concentrations greater than 25 mM (PubMed : 10837369). Also exhibits antimicrobial activity against the yeast C.albicans (PubMed : 10837369, PubMed : 30050988, PubMed : 9202117). Permeabilizes C.albicans cell membranes via targeting plasma membrane lipid phosphatidylinositol 4,5-bisphosphate (PIP2), thereby leading to cell fragmentation and cell death (PubMed : 30050988). Acts as a ligand for C-C chemokine receptor CCR6 (PubMed : 10521347, PubMed : 20068036). Binds to CCR6 and induces chemotactic activity of CCR6-expressing cells, such as immature dendritic cells and memory T cells (PubMed : 10521347, PubMed : 20068036).
See full target information DEFB4A
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