Recombinant Human beta 2 Microglobulin + FCGRT/FCRN protein (Tagged) is a Human Fragment protein, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
M S R S V A L A V L A L L S L S G L E A I Q R T P K I Q V Y S R H P A E N G K S N F L N C Y V S G F H P S D I E V D L L K N G E R I E K V E H S D L S F S K D W S F Y L L Y Y T E F T P T E K D E Y A C R V N H V T L S Q P K I V K W D R D M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).
Additional Targets
FCGRT
Alternative names
CDABP0092, HDCMA22P, B2M, Beta-2-microglobulin
Recommended products
Recombinant Human beta 2 Microglobulin + FCGRT/FCRN protein (Tagged) is a Human Fragment protein, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- His tag C-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P61769-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 5
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride
Sequence info
Amino acid sequence 1
- Accession
- P55899
- Protein length
- Fragment
- Predicted molecular weight
- 32 kDa
- Nature
- Recombinant
- Tags
- His tag C-Terminus
Amino acid sequence 2
- M S R S V A L A V L A L L S L S G L E A I Q R T P K I Q V Y S R H P A E N G K S N F L N C Y V S G F H P S D I E V D L L K N G E R I E K V E H S D L S F S K D W S F Y L L Y Y T E F T P T E K D E Y A C R V N H V T L S Q P K I V K W D R D M
- Accession
- P61769
- Protein length
- Full Length
- Predicted molecular weight
- 14 kDa
- Amino acids
- 1 to 119
- Nature
- Recombinant
Specifications
- Form
- Liquid
General info
- Function
Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).
- Sequence similarities
Belongs to the beta-2-microglobulin family.
- Post-translational modifications
Glycation of Ile-21 is observed in long-term hemodialysis patients.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta-2 microglobulin (B2M) is a component of the major histocompatibility complex (MHC) class I molecules which are integral to the immune system. It is also associated with the neonatal Fc receptor (FCGRT or FcRn). The B2M protein has a mass of approximately 11.8 kDa. This protein is widely expressed with notable expression in all nucleated cells. In humans the role of B2M includes antigen presentation to T cells and it aids in the stabilization of MHC molecules.
- Biological function summary
B2M interacts with the neonatal Fc receptor and is involved in the regulation of IgG antibody levels by extending their half-life. As part of the FcRn complex B2M also aids in the transport of IgG across epithelial barriers such as the intestinal lining in neonatal mammals. This complex plays a significant role in maintaining the homeostasis of IgG antibodies therefore impacting adaptive immunity.
- Pathways
B2M participates in antigen processing and presentation. It forms a complex with FCGRT which is critical in the FcRn pathway responsible for the recycling and transcytosis of immunoglobulins. This interaction preserves IgG from lysosomal degradation. Additionally B2M interacts with other proteins like albumin through related pathways highlighting its involvement in protein recycling and homeostasis.
- Associated diseases and disorders
B2M is relevant to several autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Altered B2M levels are indicative of these conditions reflecting immune dysregulation. Higher levels of B2M are also observed in multiple myeloma a disorder featuring malignancies of plasma cells. In these disease contexts B2M often serves as a biomarker alongside elevated levels of IgG or the presence of paraproteins.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human beta 2 Microglobulin + FCGRT/FCRN protein (Tagged) (ab271513)
SDS-PAGE analysis of ab271513.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com