Recombinant Human beta 2 Microglobulin + FCGRT/FCRN protein (Tagged) (Biotin) is a Human Full Length protein, in the 1 to 119 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE, Size Exclusion Chromatography.
Images
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE, Size Exclusion Chromatography
- Biologically active
- No
- Conjugation
- Biotin
Sequence
This product is comprised of multiple sequences see
Amino acid sequence 1
M S R S V A L A V L A L L S L S G L E A I Q R T P K I Q V Y S R H P A E N G K S N F L N C Y V S G F H P S D I E V D L L K N G E R I E K V E H S D L S F S K D W S F Y L L Y Y T E F T P T E K D E Y A C R V N H V T L S Q P K I V K W D R D M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application Size Exclusion Chromatography | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Cell surface receptor that transfers passive humoral immunity from the mother to the newborn. Binds to the Fc region of monomeric immunoglobulin gamma and mediates its selective uptake from milk (PubMed:10933786, PubMed:7964511). IgG in the milk is bound at the apical surface of the intestinal epithelium. The resultant FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids. Throughout life, contributes to effective humoral immunity by recycling IgG and extending its half-life in the circulation. Mechanistically, monomeric IgG binding to FcRn in acidic endosomes of endothelial and hematopoietic cells recycles IgG to the cell surface where it is released into the circulation (PubMed:10998088). In addition of IgG, regulates homeostasis of the other most abundant circulating protein albumin/ALB (PubMed:24469444, PubMed:28330995). (Microbial infection) Acts as an uncoating receptor for a panel of echoviruses including Echovirus 5, 6, 7, 9, 11, 13, 25 and 29.
Additional Targets
B2M
Alternative names
FCRN, FCGRT, IgG receptor FcRn large subunit p51, FcRn, IgG Fc fragment receptor transporter alpha chain, Neonatal Fc receptor
Recommended products
Recombinant Human beta 2 Microglobulin + FCGRT/FCRN protein (Tagged) (Biotin) is a Human Full Length protein, in the 1 to 119 aa range, expressed in HEK 293, with >=90% purity and suitable for SDS-PAGE, Size Exclusion Chromatography.
Key facts
- Purity
- >=90% SDS-PAGE
- Expression system
- HEK 293 cells
- Tags
- Avi tag C-Terminus His tag C-Terminus
- Applications
- SDS-PAGE, Size Exclusion Chromatography
- Biologically active
- No
- Conjugation
- Biotin
- Accession
- P55899-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 5
Constituents: 20% Glycerol (glycerin, glycerine), 0.64% Sodium chloride, 0.63% Tris HCl, 0.02% Potassium chloride
Sequence info
Amino acid sequence 1
- M S R S V A L A V L A L L S L S G L E A I Q R T P K I Q V Y S R H P A E N G K S N F L N C Y V S G F H P S D I E V D L L K N G E R I E K V E H S D L S F S K D W S F Y L L Y Y T E F T P T E K D E Y A C R V N H V T L S Q P K I V K W D R D M
- Accession
- P61769
- Protein length
- Full Length
- Predicted molecular weight
- 14 kDa
- Amino acids
- 1 to 119
- Nature
- Recombinant
Amino acid sequence 2
- A E S H L S L L Y H L T A V S S P A P G T P A F W V S G W L G P Q Q Y L S Y N S L R G E A E P C G A W V W E N Q V S W Y W E K E T T D L R I K E K L F L E A F K A L G G K G P Y T L Q G L L G C E L G P D N T S V P T A K F A L N G E E F M N F D L K Q G T W G G D W P E A L A I S Q R W Q Q Q D K A A N K E L T F L L F S C P H R L R E H L E R G R G N L E W K E P P S M R L K A R P S S P G F S V L T C S A F S F Y P P E L Q L R F L R N G L A A G T G Q G D F G P N S D G S F H A S S S L T V K S G D E H H Y C C I V Q H A G L A Q P L R V E L E S P A K S S
- Accession
- P61769
- Protein length
- Fragment
- Predicted molecular weight
- 34 kDa
- Amino acids
- 24 to 297
- Nature
- Recombinant
- Tags
- Avi tag C-Terminus, His tag C-Terminus
Specifications
- Form
- Liquid
General info
- Function
Cell surface receptor that transfers passive humoral immunity from the mother to the newborn. Binds to the Fc region of monomeric immunoglobulin gamma and mediates its selective uptake from milk (PubMed:10933786, PubMed:7964511). IgG in the milk is bound at the apical surface of the intestinal epithelium. The resultant FcRn-IgG complexes are transcytosed across the intestinal epithelium and IgG is released from FcRn into blood or tissue fluids. Throughout life, contributes to effective humoral immunity by recycling IgG and extending its half-life in the circulation. Mechanistically, monomeric IgG binding to FcRn in acidic endosomes of endothelial and hematopoietic cells recycles IgG to the cell surface where it is released into the circulation (PubMed:10998088). In addition of IgG, regulates homeostasis of the other most abundant circulating protein albumin/ALB (PubMed:24469444, PubMed:28330995).
- Sequence similarities
Belongs to the immunoglobulin superfamily.
- Subcellular localisation
- Endosome membrane
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle, Store in the dark
Notes
Enzymatically biotin-labeled using Avi-tag™ technology.
Co-expressed in HEK-293 cells.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta-2 microglobulin (B2M) is a component of the major histocompatibility complex (MHC) class I molecules which are integral to the immune system. It is also associated with the neonatal Fc receptor (FCGRT or FcRn). The B2M protein has a mass of approximately 11.8 kDa. This protein is widely expressed with notable expression in all nucleated cells. In humans the role of B2M includes antigen presentation to T cells and it aids in the stabilization of MHC molecules.
- Biological function summary
B2M interacts with the neonatal Fc receptor and is involved in the regulation of IgG antibody levels by extending their half-life. As part of the FcRn complex B2M also aids in the transport of IgG across epithelial barriers such as the intestinal lining in neonatal mammals. This complex plays a significant role in maintaining the homeostasis of IgG antibodies therefore impacting adaptive immunity.
- Pathways
B2M participates in antigen processing and presentation. It forms a complex with FCGRT which is critical in the FcRn pathway responsible for the recycling and transcytosis of immunoglobulins. This interaction preserves IgG from lysosomal degradation. Additionally B2M interacts with other proteins like albumin through related pathways highlighting its involvement in protein recycling and homeostasis.
- Associated diseases and disorders
B2M is relevant to several autoimmune diseases such as rheumatoid arthritis and systemic lupus erythematosus. Altered B2M levels are indicative of these conditions reflecting immune dysregulation. Higher levels of B2M are also observed in multiple myeloma a disorder featuring malignancies of plasma cells. In these disease contexts B2M often serves as a biomarker alongside elevated levels of IgG or the presence of paraproteins.
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2 product images
SDS-PAGE - Recombinant Human beta 2 Microglobulin + FCGRT/FCRN protein (Tagged) (Biotin) (ab271512)
SDS-PAGE analysis of ab271512.
Size Exclusion Chromatography - Recombinant Human beta 2 Microglobulin + FCGRT/FCRN protein (Tagged) (Biotin) (ab271512)
SEC analysis of ab271512.
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