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Recombinant Human beta Catenin protein (Tagged) is a Human Full Length protein, in the 1 to 781 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, ELISA, WB.
Alternative names=Catenin beta-1, Beta-catenin, CTNNB, OK/SW-cl.35, PRO2286, CTNNB1
- Purity
>70%
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
- Sequence
M A T Q A D L M E L D M A M E P D R K A A V S H W Q Q Q S Y L D S G I H S G A T T T A P S L S G K G N P E E E D V D T S Q V L Y E W E Q G F S Q S F T Q E Q V A D I D G Q Y A M T R A Q R V R A A M F P E T L D E G M Q I P S T Q F D A A H P T N V Q R L A E P S Q M L K H A V V N L I N Y Q D D A E L A T R A I P E L T K L L N D E D Q V V V N K A A V M V H Q L S K K E A S R H A I M R S P Q M V S A I V R T M Q N T N D V E T A R C T A G T L H N L S H H R E G L L A I F K S G G I P A L V K M L G S P V D S V L F Y A I T T L H N L L L H Q E G A K M A V R L A G G L Q K M V A L L N K T N V K F L A I T T D C L Q I L A Y G N Q E S K L I I L A S G G P Q A L V N I M R T Y T Y E K L L W T T S R V L K V L S V C S S N K P A I V E A G G M Q A L G L H L T D P S Q R L V Q N C L W T L R N L S D A A T K Q E G M E G L L G T L V Q L L G S D D I N V V T C A A G I L S N L T C N N Y K N K M M V C Q V G G I E A L V R T V L R A G D R E D I T E P A I C A L R H L T S R H Q E A E M A Q N A V R L H Y G L P V V V K L L H P P S H W P L I K A T V G L I R N L A L C P A N H A P L R E Q G A I P R L V Q L L V R A H Q D T Q R R T S M G G T Q Q Q F V E G V R M E E I V E G C T G A L H I L A R D V H N R I V I R G L N T I P L F V Q L L Y S P I E N I Q R V A A G V L C E L A Q D K E A A E A I E A E G A T A P L T E L L H S R N E G V A T Y A A A V L F R M S E D K P Q D Y K K R L S V E L T S S L F R T E P M A W N E T A D L G L D I G A Q G E P L G Y R Q D D P S Y R S F H S G G Y G Q D A L G M D P M M E H E M G G H H P G A D Y P V D G L P D L G H A Q D L M D G L P P G D S N Q L A W F D T D L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab63175 can be used as a WB positive control in conjunction with ab16051. |
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Recombinant Human beta Catenin protein (Tagged) is a Human Full Length protein, in the 1 to 781 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, ELISA, WB.
Alternative names=Catenin beta-1, Beta-catenin, CTNNB, OK/SW-cl.35, PRO2286, CTNNB1
Key facts
- Purity
>70%
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
- Predicted molecular weight
115 kDa
- Amino acids
1 to 781
- Sequence
M A T Q A D L M E L D M A M E P D R K A A V S H W Q Q Q S Y L D S G I H S G A T T T A P S L S G K G N P E E E D V D T S Q V L Y E W E Q G F S Q S F T Q E Q V A D I D G Q Y A M T R A Q R V R A A M F P E T L D E G M Q I P S T Q F D A A H P T N V Q R L A E P S Q M L K H A V V N L I N Y Q D D A E L A T R A I P E L T K L L N D E D Q V V V N K A A V M V H Q L S K K E A S R H A I M R S P Q M V S A I V R T M Q N T N D V E T A R C T A G T L H N L S H H R E G L L A I F K S G G I P A L V K M L G S P V D S V L F Y A I T T L H N L L L H Q E G A K M A V R L A G G L Q K M V A L L N K T N V K F L A I T T D C L Q I L A Y G N Q E S K L I I L A S G G P Q A L V N I M R T Y T Y E K L L W T T S R V L K V L S V C S S N K P A I V E A G G M Q A L G L H L T D P S Q R L V Q N C L W T L R N L S D A A T K Q E G M E G L L G T L V Q L L G S D D I N V V T C A A G I L S N L T C N N Y K N K M M V C Q V G G I E A L V R T V L R A G D R E D I T E P A I C A L R H L T S R H Q E A E M A Q N A V R L H Y G L P V V V K L L H P P S H W P L I K A T V G L I R N L A L C P A N H A P L R E Q G A I P R L V Q L L V R A H Q D T Q R R T S M G G T Q Q Q F V E G V R M E E I V E G C T G A L H I L A R D V H N R I V I R G L N T I P L F V Q L L Y S P I E N I Q R V A A G V L C E L A Q D K E A A E A I E A E G A T A P L T E L L H S R N E G V A T Y A A A V L F R M S E D K P Q D Y K K R L S V E L T S S L F R T E P M A W N E T A D L G L D I G A Q G E P L G Y R Q D D P S Y R S F H S G G Y G Q D A L G M D P M M E H E M G G H H P G A D Y P V D G L P D L G H A Q D L M D G L P P G D S N Q L A W F D T D L
- Accession
- P35222-1
- Protein length
Full Length
- Animal free
No
- Nature
Recombinant
- Species
Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Specifications
- Form
Liquid
- Additional notes
Purified by affinity chromatography
General info
- Function
Key downstream component of the canonical Wnt signaling pathway (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed:18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed:21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed:18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity).
- Sequence similarities
Belongs to the beta-catenin family.
- Post-translational modifications
Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription (By similarity). Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33. Phosphorylated by NEK2. EGF stimulates tyrosine phosphorylation. Phosphorylated on Ser-33 and Ser-37 by HIPK2 and GSK3B, this phosphorylation triggers proteasomal degradation (PubMed:25169422). Phosphorylation on Ser-191 and Ser-246 by CDK5. Phosphorylation by CDK2 regulates insulin internalization. Phosphorylation by PTK6 at Tyr-64, Tyr-142, Tyr-331 and/or Tyr-333 with the predominant site at Tyr-64 is not essential for inhibition of transcriptional activity.
- Subcellular localisation
Nucleus, Cytoskeleton, Microtubule organizing center, Centrosome, Spindle pole
Storage
- Shipped at conditions
Dry Ice
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
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