Recombinant Human beta Catenin protein (Tagged) is a Human Full Length protein, in the 1 to 781 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, ELISA, WB.
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- Nature communications 14:66822023Palmitoyltransferase DHHC9 and acyl protein thioesterase APT1 modulate renal fibrosis through regulating β-catenin palmitoylation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMengru Gu,Hanlu Jiang,Mengzhu Tan,Long Yu,Ning Xu,Ying Li,Han Wu,Qing Hou,Chunsun DaiPubMed 37865665
- eLife 11:2022Regulation of canonical Wnt signalling by the ciliopathy protein MKS1 and the E2 ubiquitin-conjugating enzyme UBE2E1.Applications:Unspecified applicationReactive species:Unspecified reactive speciesKatarzyna Szymanska,Karsten Boldt,Clare V Logan,Matthew Adams,Philip A Robinson,Marius Ueffing,Elton Zeqiraj,Gabrielle Wheway,Colin A JohnsonPubMed 35170427
- Nature communications 13:3502022Unprotected peptide macrocyclization and stapling via a fluorine-thiol displacement reaction.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMd Shafiqul Islam,Samuel L Junod,Si Zhang,Zakey Yusuf Buuh,Yifu Guan,Mi Zhao,Kishan H Kaneria,Parmila Kafley,Carson Cohen,Robert Maloney,Zhigang Lyu,Vincent A Voelz,Weidong Yang,Rongsheng E WangPubMed 35039490
- Nature 584:98-1012020Intracellular pH controls WNT downstream of glycolysis in amniote embryos.Applications:Unspecified applicationReactive species:Unspecified reactive speciesMasayuki Oginuma et. al.PubMed 32581357
- Scientific reports 8:6712018Beta-catenin cleavage enhances transcriptional activation.Applications:Unspecified applicationReactive species:Unspecified reactive speciesTatiana Goretsky et. al.PubMed 29330435
- Cancer research 76:5615-56272016RBM5-AS1 Is Critical for Self-Renewal of Colon Cancer Stem-like Cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSerena Di Cecilia,Fan Zhang,Ana Sancho,SiDe Li,Francesca Aguiló,Yifei Sun,Madhumitha Rengasamy,Weijia Zhang,Luigi Del Vecchio,Francesco Salvatore,Martin J WalshPubMed 27520449
- ACS central science 1:374-3822015Rapid, Multiplexed Phosphoprotein Profiling Using Silicon Photonic Sensor Arrays.Applications:Unspecified applicationReactive species:Unspecified reactive speciesJames H Wade,Aurora T Alsop,Nicholas R Vertin,Hongwei Yang,Mark D Johnson,Ryan C BaileyPubMed 26539563
Key facts
- Purity
>70%
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
Amino acid sequence
M A T Q A D L M E L D M A M E P D R K A A V S H W Q Q Q S Y L D S G I H S G A T T T A P S L S G K G N P E E E D V D T S Q V L Y E W E Q G F S Q S F T Q E Q V A D I D G Q Y A M T R A Q R V R A A M F P E T L D E G M Q I P S T Q F D A A H P T N V Q R L A E P S Q M L K H A V V N L I N Y Q D D A E L A T R A I P E L T K L L N D E D Q V V V N K A A V M V H Q L S K K E A S R H A I M R S P Q M V S A I V R T M Q N T N D V E T A R C T A G T L H N L S H H R E G L L A I F K S G G I P A L V K M L G S P V D S V L F Y A I T T L H N L L L H Q E G A K M A V R L A G G L Q K M V A L L N K T N V K F L A I T T D C L Q I L A Y G N Q E S K L I I L A S G G P Q A L V N I M R T Y T Y E K L L W T T S R V L K V L S V C S S N K P A I V E A G G M Q A L G L H L T D P S Q R L V Q N C L W T L R N L S D A A T K Q E G M E G L L G T L V Q L L G S D D I N V V T C A A G I L S N L T C N N Y K N K M M V C Q V G G I E A L V R T V L R A G D R E D I T E P A I C A L R H L T S R H Q E A E M A Q N A V R L H Y G L P V V V K L L H P P S H W P L I K A T V G L I R N L A L C P A N H A P L R E Q G A I P R L V Q L L V R A H Q D T Q R R T S M G G T Q Q Q F V E G V R M E E I V E G C T G A L H I L A R D V H N R I V I R G L N T I P L F V Q L L Y S P I E N I Q R V A A G V L C E L A Q D K E A A E A I E A E G A T A P L T E L L H S R N E G V A T Y A A A V L F R M S E D K P Q D Y K K R L S V E L T S S L F R T E P M A W N E T A D L G L D I G A Q G E P L G Y R Q D D P S Y R S F H S G G Y G Q D A L G M D P M M E H E M G G H H P G A D Y P V D G L P D L G H A Q D L M D G L P P G D S N Q L A W F D T D L
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab63175 can be used as a WB positive control in conjunction with Anti-beta Catenin antibody ab16051. |
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Target data
Function
Key downstream component of the canonical Wnt signaling pathway (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed:18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed:21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed:18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity).
Alternative names
Catenin beta-1, Beta-catenin, CTNNB, OK/SW-cl.35, PRO2286, CTNNB1
Recommended products
Recombinant Human beta Catenin protein (Tagged) is a Human Full Length protein, in the 1 to 781 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, ELISA, WB.
Key facts
- Purity
>70%
- Expression system
Baculovirus infected Sf9 cells
- Tags
GST tag N-Terminus
- Applications
SDS-PAGE, ELISA, WB
- Biologically active
No
- Accession
- P35222-1
- Animal free
No
- Species
Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00174% PMSF
Sequence info
Amino acid sequence
M A T Q A D L M E L D M A M E P D R K A A V S H W Q Q Q S Y L D S G I H S G A T T T A P S L S G K G N P E E E D V D T S Q V L Y E W E Q G F S Q S F T Q E Q V A D I D G Q Y A M T R A Q R V R A A M F P E T L D E G M Q I P S T Q F D A A H P T N V Q R L A E P S Q M L K H A V V N L I N Y Q D D A E L A T R A I P E L T K L L N D E D Q V V V N K A A V M V H Q L S K K E A S R H A I M R S P Q M V S A I V R T M Q N T N D V E T A R C T A G T L H N L S H H R E G L L A I F K S G G I P A L V K M L G S P V D S V L F Y A I T T L H N L L L H Q E G A K M A V R L A G G L Q K M V A L L N K T N V K F L A I T T D C L Q I L A Y G N Q E S K L I I L A S G G P Q A L V N I M R T Y T Y E K L L W T T S R V L K V L S V C S S N K P A I V E A G G M Q A L G L H L T D P S Q R L V Q N C L W T L R N L S D A A T K Q E G M E G L L G T L V Q L L G S D D I N V V T C A A G I L S N L T C N N Y K N K M M V C Q V G G I E A L V R T V L R A G D R E D I T E P A I C A L R H L T S R H Q E A E M A Q N A V R L H Y G L P V V V K L L H P P S H W P L I K A T V G L I R N L A L C P A N H A P L R E Q G A I P R L V Q L L V R A H Q D T Q R R T S M G G T Q Q Q F V E G V R M E E I V E G C T G A L H I L A R D V H N R I V I R G L N T I P L F V Q L L Y S P I E N I Q R V A A G V L C E L A Q D K E A A E A I E A E G A T A P L T E L L H S R N E G V A T Y A A A V L F R M S E D K P Q D Y K K R L S V E L T S S L F R T E P M A W N E T A D L G L D I G A Q G E P L G Y R Q D D P S Y R S F H S G G Y G Q D A L G M D P M M E H E M G G H H P G A D Y P V D G L P D L G H A Q D L M D G L P P G D S N Q L A W F D T D L
- Accession
- Protein length
Full Length
- Predicted molecular weight
115 kDa
- Amino acids
1 to 781
- Nature
Recombinant
- Tags
GST tag N-Terminus
Specifications
- Form
Liquid
- Additional notes
Purified by affinity chromatography
General info
- Function
Key downstream component of the canonical Wnt signaling pathway (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the absence of Wnt, forms a complex with AXIN1, AXIN2, APC, CSNK1A1 and GSK3B that promotes phosphorylation on N-terminal Ser and Thr residues and ubiquitination of CTNNB1 via BTRC and its subsequent degradation by the proteasome (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). In the presence of Wnt ligand, CTNNB1 is not ubiquitinated and accumulates in the nucleus, where it acts as a coactivator for transcription factors of the TCF/LEF family, leading to activate Wnt responsive genes (PubMed:17524503, PubMed:18077326, PubMed:18086858, PubMed:18957423, PubMed:21262353, PubMed:22155184, PubMed:22647378, PubMed:22699938). Involved in the regulation of cell adhesion, as component of an E-cadherin:catenin adhesion complex (By similarity). Acts as a negative regulator of centrosome cohesion (PubMed:18086858). Involved in the CDK2/PTPN6/CTNNB1/CEACAM1 pathway of insulin internalization (PubMed:21262353). Blocks anoikis of malignant kidney and intestinal epithelial cells and promotes their anchorage-independent growth by down-regulating DAPK2 (PubMed:18957423). Disrupts PML function and PML-NB formation by inhibiting RANBP2-mediated sumoylation of PML (PubMed:22155184). Promotes neurogenesis by maintaining sympathetic neuroblasts within the cell cycle (By similarity). Involved in chondrocyte differentiation via interaction with SOX9: SOX9-binding competes with the binding sites of TCF/LEF within CTNNB1, thereby inhibiting the Wnt signaling (By similarity).
- Sequence similarities
Belongs to the beta-catenin family.
- Post-translational modifications
Phosphorylation at Ser-552 by AMPK promotes stabilizion of the protein, enhancing TCF/LEF-mediated transcription (By similarity). Phosphorylation by GSK3B requires prior phosphorylation of Ser-45 by another kinase. Phosphorylation proceeds then from Thr-41 to Ser-37 and Ser-33. Phosphorylated by NEK2. EGF stimulates tyrosine phosphorylation. Phosphorylated on Ser-33 and Ser-37 by HIPK2 and GSK3B, this phosphorylation triggers proteasomal degradation (PubMed:25169422). Phosphorylation on Ser-191 and Ser-246 by CDK5. Phosphorylation by CDK2 regulates insulin internalization. Phosphorylation by PTK6 at Tyr-64, Tyr-142, Tyr-331 and/or Tyr-333 with the predominant site at Tyr-64 is not essential for inhibition of transcriptional activity.
- Subcellular localisation
Nucleus, Cytoskeleton, Microtubule organizing center, Centrosome, Spindle pole
Storage
- Shipped at conditions
Dry Ice
- Appropriate short-term storage conditions
-80°C
- Appropriate long-term storage conditions
-80°C
- Aliquoting information
Upon delivery aliquot
- Storage information
Avoid freeze / thaw cycle
Supplementary info
This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta Catenin also known by names such as CTNNB1 or beta-chip is an important protein involved in cell signaling and adhesion. This protein has a molecular weight of around 88 kDa. Beta Catenin is expressed in many cell types and tissues indicating its widespread role in various biological processes. It functions mechanically by mediating the linkage between cadherins and the actin cytoskeleton facilitating cell-cell adhesion. Beta Catenin is also a central part of transcription regulation processes in the nucleus.
- Biological function summary
This protein plays roles in both cell adhesion and the regulation of gene expression. Beta Catenin is a critical component of the Wnt signaling pathway where it can form complexes with other proteins to influence gene transcription. In the absence of Wnt signaling beta Catenin levels are low due to its degradation. However when the pathway is active it accumulates in the cytoplasm and eventually translocates to the nucleus where it interacts with TCF/LEF transcription factors to regulate the expression of target genes.
- Pathways
Beta Catenin plays a central role in the Wnt signaling pathway and influences cell fate decisions and cellular proliferation. It acts in concert with proteins such as Dishevelled (DVL) and Axin to coordinate these important biological processes. In the absence of Wnt signaling proteins such as APC and GSK-3β are responsible for beta Catenin degradation keeping its cellular levels in check. Beta Catenin’s interaction with transcription factors in the nucleus makes it pivotal in the regulation of cell and tissue homeostasis.
- Associated diseases and disorders
Beta Catenin has associations with colorectal cancer and hepatocellular carcinoma. Its dysregulation can lead to unchecked cell proliferation and tumorigenesis. Often mutations in the beta Catenin gene (CTNNB1) or components of the Wnt pathway like APC are implicated in the development of these cancers. Its interplay with E-cadherin is important for maintaining tissue architecture and disruptions can lead to invasive cancer phenotypes. Understanding beta Catenin’s role provides insights into therapeutic strategies for these cancers.
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3 product images
Western blot - Recombinant Human beta Catenin protein (Tagged) (ab63175)
All lanes: Western blot - Anti-beta Catenin antibody (Anti-beta Catenin antibody ab16051) at 0.25 µg/mL
All lanes: Western blot - Recombinant Human beta Catenin protein (Tagged) (ab63175) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 85 kDa
Exposure time: 10s
Sandwich ELISA - Recombinant Human beta Catenin protein (Tagged) (ab63175)
Standard Curve for ab63175; dilution range 1 pg/ml to 1 μg/ml using Capture Antibody Mouse monoclonal [BDI080] to beta Catenin (ab19448) at 1 μg/ml and Detector Antibody Rabbit polyclonal to beta Catenin (Anti-beta Catenin antibody ab16051) at 0.1 μg/ml.
SDS-PAGE - Recombinant Human beta Catenin protein (Tagged) (ab63175)
SDS-PAGE analysis of Recombinant Human beta Catenin protein (Tagged) (ab63175).
MW ~115 kDa
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