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AB219458

Recombinant human beta glucuronidase (GUSB) protein (Active) (His tag C-Terminus)

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(1 Publication)

Recombinant human beta glucuronidase (GUSB) protein (Active) (His tag C-Terminus) is a Human Full Length protein, in the 23 to 651 aa range, expressed in Baculovirus infected insect cells, with >90%, < 1 EU/µg endotoxin level, suitable for SDS-PAGE, FuncS.

View Alternative Names

Beta-glucuronidase, Beta-G1, GUSB

1 Images
SDS-PAGE - Recombinant human beta glucuronidase (GUSB) protein (Active) (His tag C-Terminus) (AB219458)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human beta glucuronidase (GUSB) protein (Active) (His tag C-Terminus) (AB219458)

15% SDS-PAGE using 3ug of ab219458.

Key facts

Purity

>90% SDS-PAGE

Endotoxin level

< 1 EU/µg

Expression system

Baculovirus infected insect cells

Tags

His tag C-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

Specific activity is > 4000pmol/min/ug and is defined as the amount of enzyme that hydrolyze 1pmole of 4-Methylum-belliferyl-beta-D-glucosiduronic acid to 4-Methylumbelliferone per minute at 37°C and pH3.5.

Accession

P08236

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.4 Constituents: PBS, 10% Glycerol (glycerin, glycerine)

storage-buffer

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Assay Kits

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β-Glucuronidase Activity Assay Kit (Fluorometric)

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Primary Antibodies

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Anti-beta glucuronidase (GUSB) antibody [EPR10616]

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"LQGGMLYPQESPSRECKELDGLWSFRADFSDNRRRGFEEQWYRRPLWESGPTVDMPVPSSFNDISQDWRLRHFVGWVWYEREVILPERWTQDLRTRVVLRIGSAHSYAIVWVNGVDTLEHEGGYLPFEADISNLVQVGPLPSRLRITIAINNTLTPTTLPPGTIQYLTDTSKYPKGYFVQNTYFDFFNYAGLQRSVLLYTTPTTYIDDITVTTSVEQDSGLVNYQISVKGSNLFKLEVRLLDAENKVVANGTGTQGQLKVPGVSLWWPYLMHERPAYLYSLEVQLTAQTSLGPVSDFYTLPVGIRTVAVTKSQFLINGKPFYFHGVNKHEDADIRGKGFDWPLLVKDFNLLRWLGANAFRTSHYPYAEEVMQMCDRYGIVVIDECPGVGLALPQFFNNVSLHHHMQVMEEVVRRDKNHPAVVMWSVANEPASHLESAGYYLKMVIAHTKSLDPSRPVTFVSNSNYAADKGAPYVDVICLNSYYSWYHDYGHLELIQLQLATQFENWYKKYQKPIIQSEYGAETIAGFHQDPPLMFTEEYQKSLLEQYHLGLDQKRRKYVVGELIWNFADFMTEQSPTRVLGNKKGIFTRQRQPKSAAFLLRERYWKIANETRYPHSVAKSQCLENSLFTHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"73.4 kDa","actualMolecularWeight":null,"aminoAcidEnd":651,"aminoAcidStart":23,"nature":"Recombinant","expressionSystem":"Baculovirus infected insect cells","accessionNumber":"P08236","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Beta glucuronidase also known as GUSB is an enzyme with several well-known names including b glucuronidase b-glucuronidase and beta-glucuronidase. It is a hydrolase enzyme with a molecular mass around 75 kDa. The enzyme is widely expressed in lysosomes of various tissues such as liver spleen and kidney. Mechanically beta-glucuronidase cleaves beta-D-glucuronic acid residues from glycosaminoglycans steroids and hormones an essential step for the degradation of glycosaminoglycans.
Biological function summary

Beta glucuronidase performs significant roles in cellular metabolism and molecule recycling. This enzyme aids in the breakdown and recycling of complex carbohydrates. By facilitating hydrolysis it helps in maintaining cellular homeostasis. Beta glucuronidase is not part of a complex; it functions independently within lysosomes. Its activity is vital for recycling endocytosed material and managing cellular turnover.

Pathways

Beta glucuronidase plays an important role in the lysosomal degradation pathway. This enzyme aids in glycosaminoglycan catabolism a process important for cellular waste disposal and recycling. Its activity connects with several proteins in the pathway notably those involved in lysosomal function such as Hexosaminidase. Beta glucuronidase's enzymatic function further integrates into the broader context of carbohydrate metabolism pathways enabling efficient breakdown of large carbohydrate molecules.

Mutations or deficiencies in beta glucuronidase lead to mucopolysaccharidosis type VII also called Sly syndrome. This lysosomal storage disorder results in the accumulation of undegraded glycosaminoglycans causing developmental delay skeletal abnormalities and other systemic issues. Additionally beta glucuronidase contributes to cancer biology where altered expressions can influence tumor progression. Proteins like glucocerebrosidase may interact indirectly impacting the disease's pathophysiology.
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Specifications

Form

Liquid

Additional notes

Affinity purified

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General info

Function

Plays an important role in the degradation of dermatan and keratan sulfates.

Sequence similarities

Belongs to the glycosyl hydrolase 2 family.

Post-translational modifications

N-linked glycosylated with 3 to 4 oligosaccharide chains.

Subcellular localisation

Lysosome

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Product protocols

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Target data

Plays an important role in the degradation of dermatan and keratan sulfates.
See full target information GUSB
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Molecular metabolism 83:101930 PubMed38570069

2024

Tumoral acidosis promotes adipose tissue depletion by fostering adipocyte lipolysis.

Applications

Unspecified application

Species

Unspecified reactive species

Camille Lefevre,Morgane M Thibaut,Audrey Loumaye,Jean-Paul Thissen,Audrey M Neyrinck,Benoit Navez,Nathalie M Delzenne,Olivier Feron,Laure B Bindels
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Product promise

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