Recombinant Human beta I Tubulin protein is a Human Full Length protein, in the 1 to 451 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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- Cell death & disease 12:422021TRIB2 modulates proteasome function to reduce ubiquitin stability and protect liver cancer cells against oxidative stress.Applications:Unspecified applicationReactive species:Unspecified reactive speciesSusu Guo et. al.PubMed 33414446
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M R E I V H I Q I G Q C G N Q I G A K F W E M I G E E H G I D L A G S D R G A S A L Q L E R I S V Y Y N E A Y G R K Y V P R A V L V D L E P G T M D S I R S S K L G A L F Q P D S F V H G N S G A G N N W A K G H Y T E G A E L I E N V L E V V R H E S E S C D C L Q G F Q I V H S L G G G T G S G M G T L L M N K I R E E Y P D R I M N S F S V M P S P K V S D T V V E P Y N A V L S I H Q L I E N A D A C F C I D N E A L Y D I C F R T L K L T T P T Y G D L N H L V S L T M S G I T T S L R F P G Q L N A D L R K L A V N M V P F P R L H F F M P G F A P L T A Q G S Q Q Y R A L S V A E L T Q Q M F D A R N T M A A C D L R R G R Y L T V A C I F R G K M S T K E V D Q Q L L S V Q T R N S S C F V E W I P N N V K V A V C D I P P R G L S M A A T F I G N N T A I Q E I F N R V S E H F S A M F K R K A F V H W Y T S E G M D I N E F G E A E N N I H D L V S E Y Q Q F Q D A K A V L E E D E E V T E E A E M E P E D K G H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Alternative names
Tubulin beta-1 chain, TUBB1
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Recombinant Human beta I Tubulin protein is a Human Full Length protein, in the 1 to 451 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- Q9H4B7-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M R E I V H I Q I G Q C G N Q I G A K F W E M I G E E H G I D L A G S D R G A S A L Q L E R I S V Y Y N E A Y G R K Y V P R A V L V D L E P G T M D S I R S S K L G A L F Q P D S F V H G N S G A G N N W A K G H Y T E G A E L I E N V L E V V R H E S E S C D C L Q G F Q I V H S L G G G T G S G M G T L L M N K I R E E Y P D R I M N S F S V M P S P K V S D T V V E P Y N A V L S I H Q L I E N A D A C F C I D N E A L Y D I C F R T L K L T T P T Y G D L N H L V S L T M S G I T T S L R F P G Q L N A D L R K L A V N M V P F P R L H F F M P G F A P L T A Q G S Q Q Y R A L S V A E L T Q Q M F D A R N T M A A C D L R R G R Y L T V A C I F R G K M S T K E V D Q Q L L S V Q T R N S S C F V E W I P N N V K V A V C D I P P R G L S M A A T F I G N N T A I Q E I F N R V S E H F S A M F K R K A F V H W Y T S E G M D I N E F G E A E N N I H D L V S E Y Q Q F Q D A K A V L E E D E E V T E E A E M E P E D K G H
- Accession
- Q9H4B7
- Protein length
- Full Length
- Amino acids
- 1 to 451
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
- Sequence similarities
Belongs to the tubulin family.
- Post-translational modifications
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Glutamylation is also involved in cilia motility (By similarity).
- Subcellular localisation
- Cytoskeleton
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta I Tubulin also known as TUBB1 is an essential component of the microtubule cytoskeleton specifically expressed in hematopoietic cells such as platelets. It is a protein with a mass of approximately 50 kDa. This protein shares structural similarities with other beta tubulins forming heterodimers with alpha-tubulin to construct the microtubules necessary for cell shape and movement. Beta I Tubulin primarily localizes to regions where it aids in the dynamics of microtubule assembly and stabilization.
- Biological function summary
Beta tubulins are important for proper cell division signal transduction and organelle positioning. Beta I Tubulin integrates into the microtubule complex playing an important role in platelet formation and function. The dynamic behavior of microtubules facilitated by beta I Tubulin and its interactions influences cytoskeletal organization enabling cells to maintain their architecture and transport intracellular components effectively.
- Pathways
Beta I Tubulin becomes critical in signaling pathways that involve cytoskeleton dynamics such as the Rho GTPase signaling pathway which is essential for actin and microtubule dynamics. It works together with proteins like alpha-tubulin and actin to ensure the proper development of megakaryocytes precursors to platelets. The function of these pathways determines efficient platelet production and stabilization closely linked to overall cellular homeostasis and response to external stimuli.
- Associated diseases and disorders
Beta I Tubulin has connections to conditions such as congenital macrothrombocytopenia and other platelet disorders. Mutations or dysregulations of beta I Tubulin can lead to abnormal platelet morphology and function impacting hemostasis. This protein also shows connections to alpha-tubulin in these disorders indicating the essential partnership between tubulin subunits in the formation of functional microtubules and emphasizing their significance in maintaining normal physiological states.
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1 product image
SDS-PAGE - Recombinant Human beta I Tubulin protein (ab164310)
ab164310 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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