Recombinant Human beta II Tubulin protein is a Human Full Length protein, in the 1 to 445 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Images
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
M R E I V H L Q A G Q C G N Q I G A K F W E V I S D E H G I D P T G T Y H G D S D L Q L E R I N V Y Y N E A T G G K Y V P R A V L V D L E P G T M D S V R S G P F G Q I F R P D N F V F G Q S G A G N N W A K G H Y T E G A E L V D S V L D V V R K E A E S C D C L Q G F Q L T H S L G G G T G S G M G T L L I S K I R E E Y P D R I M N T F S V V P S P K V S D T V V E P Y N A T L S V H Q L V E N T D E T Y C I D N E A L Y D I C F R T L K L T T P T Y G D L N N L V S A T M S G V T T C L R F P G Q L N A D L R K L A V N M V P F P R L H F F M P G F A P L T S R G S Q Q Y R A L T V P E L T Q Q M F D A K N M M A A C D P R H G R Y L T V A A V F R G R M S M K E V D E Q M L N V Q N K N S S Y F V E W I P N N V K T A V C D I P P R G L K M S A T F I G N S T A I Q E L F K R I S E Q F T A M F R R K A F L H W Y T G E G M D E M E F T E A E S N M N D L V S E Y Q Q Y Q D A T A E E E G E F E E E A E E E V A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
Alternative names
TUBB2C, TUBB4B, Tubulin beta-4B chain, Tubulin beta-2 chain, Tubulin beta-2C chain
Recommended products
Recombinant Human beta II Tubulin protein is a Human Full Length protein, in the 1 to 445 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P68371-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- M R E I V H L Q A G Q C G N Q I G A K F W E V I S D E H G I D P T G T Y H G D S D L Q L E R I N V Y Y N E A T G G K Y V P R A V L V D L E P G T M D S V R S G P F G Q I F R P D N F V F G Q S G A G N N W A K G H Y T E G A E L V D S V L D V V R K E A E S C D C L Q G F Q L T H S L G G G T G S G M G T L L I S K I R E E Y P D R I M N T F S V V P S P K V S D T V V E P Y N A T L S V H Q L V E N T D E T Y C I D N E A L Y D I C F R T L K L T T P T Y G D L N N L V S A T M S G V T T C L R F P G Q L N A D L R K L A V N M V P F P R L H F F M P G F A P L T S R G S Q Q Y R A L T V P E L T Q Q M F D A K N M M A A C D P R H G R Y L T V A A V F R G R M S M K E V D E Q M L N V Q N K N S S Y F V E W I P N N V K T A V C D I P P R G L K M S A T F I G N S T A I Q E L F K R I S E Q F T A M F R R K A F L H W Y T G E G M D E M E F T E A E S N M N D L V S E Y Q Q Y Q D A T A E E E G E F E E E A E E E V A
- Accession
- P68371
- Protein length
- Full Length
- Amino acids
- 1 to 445
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.
- Sequence similarities
Belongs to the tubulin family.
- Post-translational modifications
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Glutamylation is also involved in cilia motility (By similarity).
- Subcellular localisation
- Cytoskeleton
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as TUBB4B.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Beta II tubulin also known as beta 2 tubulin is a significant component of microtubules which are essential for maintaining cell structure and integrity. This protein with an approximate molecular mass of 50 kDa is encoded by the TUBB2B gene. It is expressed in various tissues with high expression levels in neuronal tissues contributing to the formation of the cytoskeleton by polymerizing with alpha tubulin to form heterodimers. Alternative names for this protein include 7b9 and C#7b9 though beta II is a common reference in scientific literature.
- Biological function summary
Beta II tubulin plays an important role in cellular processes like cell division and intracellular transport. As part of the microtubule complex it provides critical support in mitotic spindle formation facilitating chromosomal segregation during mitosis. It also assists in maintaining neuronal cell stability which is vital for proper neurological functions. The dynamic nature of microtubules aided by beta II tubulin ensures the correct distribution of organelles and other intracellular structures highlighting its importance in cellular mechanics.
- Pathways
Beta II tubulin interacts with several critical cellular pathways including the mitotic spindle checkpoint pathway and axonal guidance. It closely associates with other tubulins such as alpha and gamma tubulin ensuring the regulation and stabilization of microtubule dynamics. These pathways are integral to cell cycle regulation and neural development highlighting beta II tubulin's involvement in important cellular and developmental processes.
- Associated diseases and disorders
Mutations or dysregulation in beta II tubulin can relate to neurological disorders such as malformation of cortical development and certain types of brain atrophy. These are often linked to the protein's key role in neuronal function and axonal transport. Additionally altered expression of beta II tubulin has a connection to microtubule-related proteins such as tau which are implicated in neurodegenerative disorders like Alzheimer's disease. This highlights the importance of beta II tubulin in maintaining neuronal health and provides potential targets for therapeutic interventions.
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1 product image
SDS-PAGE - Recombinant Human beta II Tubulin protein (ab160968)
ab160968 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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