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Recombinant Human beta III Tubulin protein is a Human Full Length protein, in the 1 to 450 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

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Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

Biologically active

No

Sequence

M G S S H H H H H H S S G L V P R G S H M G S M R E I V H I Q A G Q C G N Q I G A K F W E V I S D E H G I D P S G N Y V G D S D L Q L E R I S V Y Y N E A S S H K Y V P R A I L V D L E P G T M D S V R S G A F G H L F R P D N F I F G Q S G A G N N W A K G H Y T E G A E L V D S V L D V V R K E C E N C D C L Q G F Q L T H S L G G G T G S G M G T L L I S K V R E

E Y P D R I M N T F S V V P S P K V S D T V V E P Y N A T L S I H Q L V E N T D E T Y C I D N E A L Y D I C F R T L K L A T P T Y G D L N H L V S A T M S G V T T S L R F P G Q L N A D L R K L A V N M V P F P R L H F F M P G F A P L T A R G S Q Q Y R A L T V P E L T Q Q M F D A K N M M A A C D P R H G R Y L T V A T V F R G R M S M K E V D E Q M L A I Q S K N S S Y F V E W I P N N V K V A V C D I P P R G L K M S S T F I G N S T A I Q E L F K R I S E Q F T A M F R R K A F L H W Y T G E G M D E M E F T E A E S N M N D L V S E Y Q Q Y Q D A T A E E E G E M Y E D D E E E S E A Q G P K

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Reactivity data

Application
SDS-PAGE
Reactivity
Reacts
Dilution info
-
Notes

-

Images

Target data

Recommended products

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Recombinant Human beta III Tubulin protein is a Human Full Length protein, in the 1 to 450 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE

Predicted molecular weight

52.8 kDa

Amino acids

1 to 450

Sequence

M G S S H H H H H H S S G L V P R G S H M G S M R E I V H I Q A G Q C G N Q I G A K F W E V I S D E H G I D P S G N Y V G D S D L Q L E R I S V Y Y N E A S S H K Y V P R A I L V D L E P G T M D S V R S G A F G H L F R P D N F I F G Q S G A G N N W A K G H Y T E G A E L V D S V L D V V R K E C E N C D C L Q G F Q L T H S L G G G T G S G M G T L L I S K V R E

E Y P D R I M N T F S V V P S P K V S D T V V E P Y N A T L S I H Q L V E N T D E T Y C I D N E A L Y D I C F R T L K L A T P T Y G D L N H L V S A T M S G V T T S L R F P G Q L N A D L R K L A V N M V P F P R L H F F M P G F A P L T A R G S Q Q Y R A L T V P E L T Q Q M F D A K N M M A A C D P R H G R Y L T V A T V F R G R M S M K E V D E Q M L A I Q S K N S S Y F V E W I P N N V K V A V C D I P P R G L K M S S T F I G N S T A I Q E L F K R I S E Q F T A M F R R K A F L H W Y T G E G M D E M E F T E A E S N M N D L V S E Y Q Q Y Q D A T A E E E G E M Y E D D E E E S E A Q G P K

Accession
Q13509-1
Protein length

Full Length

Animal free

No

Nature

Recombinant

Species

Human

Concentration
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Storage buffer

pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

Specifications

Form

Liquid

Additional notes

ab140581 is purified using conventional chromatography techniques.

General info

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).

Sequence similarities

Belongs to the tubulin family.

Post-translational modifications

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Glutamylation is also involved in cilia motility (By similarity).

Subcellular localisation

Cytoskeleton

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Product promise

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Downloads

Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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