Sample Prep & Detection Kits
Conjugation kitsPurification kitsSample preparation kitsChromogen kitsIHC kitsChIP kitsAccessory Reagents & Controls
Accessory reagents & controlsBiochemicals
BiochemicalsProteins and Peptides
Proteins and peptidesOur latest ELISA kit: Human Tau (phospho T217) - Intracellular
Highly sensitive kit offering the most promising biomarkers for Alzheimer’s disease diagnostics. Learn about all product ranges with our product overviews.
Featured events
Make new connections at our global events.
Our programs
New Lab Program
Get a head start with our exclusive new lab discount. Enjoy 20% off and free shipping for three months.
New Biotech Program
Just starting out? Get 15% off and free shipping to your lab for six months.
Product promise
Peace of mind that all products perform as stated.
Product reviews
Leave reviews, get rewarded and help your community.
Trial program
Try untested species and applications to earn money off your next order.
Recombinant Human beta III Tubulin protein is a Human Full Length protein, in the 1 to 450 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Alternative names=Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB4, TUBB3
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE
No
M G S S H H H H H H S S G L V P R G S H M G S M R E I V H I Q A G Q C G N Q I G A K F W E V I S D E H G I D P S G N Y V G D S D L Q L E R I S V Y Y N E A S S H K Y V P R A I L V D L E P G T M D S V R S G A F G H L F R P D N F I F G Q S G A G N N W A K G H Y T E G A E L V D S V L D V V R K E C E N C D C L Q G F Q L T H S L G G G T G S G M G T L L I S K V R E
E Y P D R I M N T F S V V P S P K V S D T V V E P Y N A T L S I H Q L V E N T D E T Y C I D N E A L Y D I C F R T L K L A T P T Y G D L N H L V S A T M S G V T T S L R F P G Q L N A D L R K L A V N M V P F P R L H F F M P G F A P L T A R G S Q Q Y R A L T V P E L T Q Q M F D A K N M M A A C D P R H G R Y L T V A T V F R G R M S M K E V D E Q M L A I Q S K N S S Y F V E W I P N N V K V A V C D I P P R G L K M S S T F I G N S T A I Q E L F K R I S E Q F T A M F R R K A F L H W Y T G E G M D E M E F T E A E S N M N D L V S E Y Q Q Y Q D A T A E E E G E M Y E D D E E E S E A Q G P K
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Recombinant Human beta III Tubulin protein is a Human Full Length protein, in the 1 to 450 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Alternative names=Tubulin beta-3 chain, Tubulin beta-4 chain, Tubulin beta-III, TUBB4, TUBB3
>90% SDS-PAGE
Escherichia coli
His tag N-Terminus
SDS-PAGE
No
52.8 kDa
1 to 450
M G S S H H H H H H S S G L V P R G S H M G S M R E I V H I Q A G Q C G N Q I G A K F W E V I S D E H G I D P S G N Y V G D S D L Q L E R I S V Y Y N E A S S H K Y V P R A I L V D L E P G T M D S V R S G A F G H L F R P D N F I F G Q S G A G N N W A K G H Y T E G A E L V D S V L D V V R K E C E N C D C L Q G F Q L T H S L G G G T G S G M G T L L I S K V R E
E Y P D R I M N T F S V V P S P K V S D T V V E P Y N A T L S I H Q L V E N T D E T Y C I D N E A L Y D I C F R T L K L A T P T Y G D L N H L V S A T M S G V T T S L R F P G Q L N A D L R K L A V N M V P F P R L H F F M P G F A P L T A R G S Q Q Y R A L T V P E L T Q Q M F D A K N M M A A C D P R H G R Y L T V A T V F R G R M S M K E V D E Q M L A I Q S K N S S Y F V E W I P N N V K V A V C D I P P R G L K M S S T F I G N S T A I Q E L F K R I S E Q F T A M F R R K A F L H W Y T G E G M D E M E F T E A E S N M N D L V S E Y Q Q Y Q D A T A E E E G E M Y E D D E E E S E A Q G P K
Full Length
No
Recombinant
Human
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.32% Tris HCl, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Liquid
ab140581 is purified using conventional chromatography techniques.
Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers (PubMed:34996871). Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms (PubMed:34996871). Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin (PubMed:34996871). TUBB3 plays a critical role in proper axon guidance and maintenance (PubMed:20074521). Binding of NTN1/Netrin-1 to its receptor UNC5C might cause dissociation of UNC5C from polymerized TUBB3 in microtubules and thereby lead to increased microtubule dynamics and axon repulsion (PubMed:28483977). Plays a role in dorsal root ganglion axon projection towards the spinal cord (PubMed:28483977).
Belongs to the tubulin family.
Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Glutamylation is also involved in cilia motility (By similarity).
Cytoskeleton
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com