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Recombinant Human beta Tubulin protein is a Human Full Length protein, in the 1 to 444 aa range, expressed in Escherichia coli, with >70% purity and suitable for SDS-PAGE, WB.

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Images

SDS-PAGE - Recombinant Human beta Tubulin protein (AB70187), expandable thumbnail
  • Western blot - Recombinant Human beta Tubulin protein (AB70187), expandable thumbnail
  • Western blot - Recombinant Human beta Tubulin protein (AB70187), expandable thumbnail

Publications

Key facts

Purity

>70% Densitometry

Expression system

Escherichia coli

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB

Biologically active

No

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

WB

Reactivity

Reacts

Dilution info

-

Notes

ab70187 can be used as a WB positive control in conjunction with Anti-beta Tubulin antibody ab15568.

Target data

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Alternative names

Recommended products

Recombinant Human beta Tubulin protein is a Human Full Length protein, in the 1 to 444 aa range, expressed in Escherichia coli, with >70% purity and suitable for SDS-PAGE, WB.

Key facts

Purity

>70% Densitometry

Expression system

Escherichia coli

Applications

SDS-PAGE, WB

Accession
P07437-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Sequence info

Amino acid sequence

Accession

P07437

Protein length

Full Length

Predicted molecular weight

76 kDa

Amino acids

1 to 444

Nature

Recombinant

Tags

GST tag N-Terminus

Specifications

Form

Liquid

Additional notes

Purity is lot specific. Please contact our technical Support team for details.

General info

Function

Tubulin is the major constituent of microtubules, a cylinder consisting of laterally associated linear protofilaments composed of alpha- and beta-tubulin heterodimers. Microtubules grow by the addition of GTP-tubulin dimers to the microtubule end, where a stabilizing cap forms. Below the cap, tubulin dimers are in GDP-bound state, owing to GTPase activity of alpha-tubulin.

Sequence similarities

Belongs to the tubulin family.

Post-translational modifications

Some glutamate residues at the C-terminus are polyglutamylated, resulting in polyglutamate chains on the gamma-carboxyl group (PubMed:26875866, PubMed:28576883). Polyglutamylation plays a key role in microtubule severing by spastin (SPAST). SPAST preferentially recognizes and acts on microtubules decorated with short polyglutamate tails: severing activity by SPAST increases as the number of glutamates per tubulin rises from one to eight, but decreases beyond this glutamylation threshold (PubMed:26875866). Glutamylation is also involved in cilia motility (By similarity).

Subcellular localisation

Cytoskeleton

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

Beta tubulin also known as beta-tubulin or β-tubulin is a globular protein with a molecular weight of approximately 50 kDa. It is a major component of microtubules which are cylindrical structures vital for maintaining cell shape and enabling intracellular transport. This protein is expressed widely in eukaryotic cells and plays a role in mitosis and meiosis by forming the spindle apparatus important for chromosome separation during cell division. The size of beta tubulin allows it to effectively polymerize with alpha tubulin forming heterodimers which are the building blocks of the microtubule network.

Biological function summary

This protein contributes significantly to the dynamic instability of microtubules allowing rapid assembly and disassembly which is essential for cellular processes like motility signaling and maintaining the cell’s architecture. Beta tubulin operates as part of the tubulin family which includes several related proteins within microtubule structures. The beta-tubulin molecules in microtubules are critical for the interactions with microtubule-associated proteins (MAPs) and motor proteins such as kinesin and dynein influencing trafficking and positioning of organelles within the cell.

Pathways

Beta tubulin plays key roles in the mitotic spindle assembly checkpoint ensuring accurate chromosome segregation. It is actively involved in the microtubule pathway and has associations with signaling pathways such as the Wnt signaling pathway which affects cell growth and differentiation. Through these pathways beta tubulin interacts with proteins like tau and MAP2 which stabilize microtubules to control their functional dynamics within the cell.

Associated diseases and disorders

Mutations or dysregulations in beta tubulin can affect neurological and proliferative conditions such as neurodegenerative diseases and cancer. Beta tubulin is notably linked to Alzheimer's disease where tau protein tangles disrupt normal microtubule function. It also connects to diseases like paclitaxel-resistant cancer where altered beta tubulin isoform expression can lead to chemotherapy resistance. The interaction of beta tubulin with tau and alpha tubulin provides further insight into etiological mechanisms of these conditions.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

3 product images

  • SDS-PAGE - Recombinant Human beta Tubulin protein (ab70187), expandable thumbnail

    SDS-PAGE - Recombinant Human beta Tubulin protein (ab70187)

  • Western blot - Recombinant Human beta Tubulin protein (ab70187), expandable thumbnail

    Western blot - Recombinant Human beta Tubulin protein (ab70187)

    All lanes: Western blot - Anti-beta Tubulin antibody (Anti-beta Tubulin antibody ab15568) at 1 µg/mL

    All lanes: Western blot - Recombinant Human beta Tubulin protein (ab70187) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa

    Exposure time: 30s

  • Western blot - Recombinant Human beta Tubulin protein (ab70187), expandable thumbnail

    Western blot - Recombinant Human beta Tubulin protein (ab70187)

    All lanes: Western blot - Anti-beta Tubulin antibody (Anti-beta Tubulin antibody ab15568) at 1 µg/mL

    All lanes: Western blot - Recombinant Human beta Tubulin protein (ab70187) at 0.1 µg

    Secondary

    All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 49 kDa

    Exposure time: 30s

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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