Recombinant Human BHMT protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W G S H M P P V G G K K A K K G I L E R L N A G E I V I G D G G F V F A L E K R G Y V K A G P W T P E A A V E H P E A V R Q L H R E F L R A G S N V M Q T F T F Y A S E D K L E N R G N Y V L E K I S G Q E V N E A A C D I A R Q V A D E G D A L V A G G V S Q T P S Y L S C K S E T E V K K V F L Q Q L E V F M K K N V D F L I A E Y F E H V E E A V W A V E T L I A S G K P V A A T M C I G P E G D L H G V P P G E C A V R L V K A G A S I I G V N C H F D P T I S L K T V K L M K E G L E A A R L K A H L M S Q P L A Y H T P D C N K Q G F I D L P E F P F G L E P R V A T R W D I Q K Y A R E A Y N L G V R Y I G G C C G F E P Y H I R A I A E E L A P E R G F L P P A S E K H G S W G S G L D M H T K P W V R A R A R K E Y W E N L R I A S G R P Y N P S M S K P D G W G V T K G T A E L M Q Q K E A T T E Q Q L K E L F E K Q K F K S Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Alternative names
Betaine--homocysteine S-methyltransferase 1, BHMT
Recommended products
Recombinant Human BHMT protein is a Human Full Length protein, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q93088-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 20% Glycerol (glycerin, glycerine), 0.242% Tris
Sequence info
Amino acid sequence
- M R G S H H H H H H G M A S M T G G Q Q M G R D L Y D D D D K D R W G S H M P P V G G K K A K K G I L E R L N A G E I V I G D G G F V F A L E K R G Y V K A G P W T P E A A V E H P E A V R Q L H R E F L R A G S N V M Q T F T F Y A S E D K L E N R G N Y V L E K I S G Q E V N E A A C D I A R Q V A D E G D A L V A G G V S Q T P S Y L S C K S E T E V K K V F L Q Q L E V F M K K N V D F L I A E Y F E H V E E A V W A V E T L I A S G K P V A A T M C I G P E G D L H G V P P G E C A V R L V K A G A S I I G V N C H F D P T I S L K T V K L M K E G L E A A R L K A H L M S Q P L A Y H T P D C N K Q G F I D L P E F P F G L E P R V A T R W D I Q K Y A R E A Y N L G V R Y I G G C C G F E P Y H I R A I A E E L A P E R G F L P P A S E K H G S W G S G L D M H T K P W V R A R A R K E Y W E N L R I A S G R P Y N P S M S K P D G W G V T K G T A E L M Q Q K E A T T E Q Q L K E L F E K Q K F K S Q
- Accession
- Q93088
- Protein length
- Full Length
- Predicted molecular weight
- 49.2 kDa
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
This protein was purified by using conventional chromatography techniques.
General info
- Function
Involved in the regulation of homocysteine metabolism. Converts betaine and homocysteine to dimethylglycine and methionine, respectively. This reaction is also required for the irreversible oxidation of choline.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
SDS-PAGE - Recombinant Human BHMT protein (ab51280)
ab51280 run on a 15% SDS-PAGE gel with molecular weight markers.
Western blot - Recombinant Human BHMT protein (ab51280)
Blocking/Diluting buffer and concentration: 5% NFDM/TBST
Lane 1 and 2 samples loaded at 10 ng.
Exposure time: 58 seconds.
The blot was developed on a BIO-RAD® ChemiDoc™ MP instrument.All lanes: Western blot - Anti-BHMT + BHMT2 antibody [EPR20822] (Anti-BHMT + BHMT2 antibody [EPR20822] ab213491) at 1/1000 dilution
Lane 1: Western blot - Recombinant Human BHMT2 protein (denatured) (Recombinant Human BHMT2 protein (denatured) ab180277)
Lane 2: Western blot - Recombinant Human BHMT protein (ab51280)
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) (Goat Anti-Rabbit IgG H&L (HRP) ab97051) at 1/100000 dilution
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Product protocols
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