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AB137149

Recombinant Human Bif-1 protein

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Recombinant Human Bif-1 protein is a Human Full Length protein, in the 1 to 365 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

KIAA0491, CGI-61, SH3GLB1, Endophilin-B1, Bax-interacting factor 1, SH3 domain-containing GRB2-like protein B1, Bif-1

1 Images
SDS-PAGE - Recombinant Human Bif-1 protein (AB137149)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Bif-1 protein (AB137149)

15% SDS-PAGE analysis of 3 µg ab137149.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag C-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

Q9Y371

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

Previously labelled as Bif.
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Sequence info

[{"sequence":"MNIMDFNVKKLAADAGTFLSRAVQFTEEKLGQAEKTELDAHLENLLSKAECTKIWTEKIMKQTEVLLQPNPNARIEEFVYEKLDRKAPSRINNPELLGQYMIDAGTEFGPGTAYGNALIKCGETQKRIGTADRELIQTSALNFLTPLRNFIEGDYKTIAKERKLLQNKRLDLDAAKTRLKKAKAAETRNSSEQELRITQSEFDRQAEITRLLLEGISSTHAHHLRCLNDFVEAQMTYYAQCYQYMLDLQKQLGSFPSNYLSNNNQTSVTPVPSVLPNAIGSSAMASTSGLVITSPSNLSDLKECSGSRKARVLYDYDAANSTELSLLADEVITVFSVVGMDSDWLMGERGNQKGKVPITYLELLNLEHHHHHH","proteinLength":"Full Length","predictedMolecularWeight":"41.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":365,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y371","tags":[{"tag":"His","terminus":"C-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bif-1 also known as BAX-interacting factor-1 is a protein involved in the regulation of apoptosis and autophagy. It has an approximate mass of 23 kDa and is expressed in various tissues including the brain and immune cells. The protein is an important player in mitochondrial dynamics participating in membrane curvature required for mitochondrial fission.
Biological function summary

This protein participates in mitochondrial fragmentation and membrane remodeling. Bif-1 is a component of the endophilin B1 complex which is involved in the regulation of apoptosis. It interacts directly with BAX a pro-apoptotic protein and enhances BAX translocation to mitochondria thereby facilitating the release of cytochrome c and subsequent cell death processes. It also influences autophagic processes by binding to Beclin1.

Pathways

Bif-1 functions as an important link between the apoptosis and autophagy pathways. It facilitates the intrinsic apoptotic signaling pathway where BAX and Beclin1 play significant roles. In these pathways the protein controls cellular homeostasis and stress responses linking to other key regulators such as Bak and p53 which are involved in tumor suppression and DNA damage responses.

Bif-1 has connections to neurodegenerative diseases and cancer. Its dysregulation is associated with impaired autophagy and apoptosis contributing to the pathogenesis of Alzheimer's disease. In cancers altered expression of Bif-1 affects tumor growth and resistance to cell death interacting with proteins such as p53 and BAX which are pivotal in controlling cellular proliferation and survival.
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Specifications

Form

Liquid

Additional notes

ab137149 is purified using conventional chromatography techniques.

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General info

Function

May be required for normal outer mitochondrial membrane dynamics (PubMed : 15452144). Required for coatomer-mediated retrograde transport in certain cells (By similarity). May recruit other proteins to membranes with high curvature. May promote membrane fusion (PubMed : 11604418). Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed : 16227588). Isoform 1 acts proapoptotic in fibroblasts (By similarity). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2) (PubMed : 17891140). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation (PubMed : 21068542). Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed : 20643123). Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability (By similarity).

Sequence similarities

Belongs to the endophilin family.

Post-translational modifications

Phosphorylated at Thr-145 by CDK5; this phosphorylation is required for autophagy induction in starved neurons and facilitates homodimerization.

Subcellular localisation

Mitochondrion outer membrane

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Product protocols

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Target data

May be required for normal outer mitochondrial membrane dynamics (PubMed : 15452144). Required for coatomer-mediated retrograde transport in certain cells (By similarity). May recruit other proteins to membranes with high curvature. May promote membrane fusion (PubMed : 11604418). Involved in activation of caspase-dependent apoptosis by promoting BAX/BAK1 activation (PubMed : 16227588). Isoform 1 acts proapoptotic in fibroblasts (By similarity). Involved in caspase-independent apoptosis during nutrition starvation and involved in the regulation of autophagy. Activates lipid kinase activity of PIK3C3 during autophagy probably by associating with the PI3K complex II (PI3KC3-C2) (PubMed : 17891140). Associated with PI3KC3-C2 during autophagy may regulate the trafficking of ATG9A from the Golgi complex to the peripheral cytoplasm for the formation of autophagosomes by inducing Golgi membrane tubulation and fragmentation (PubMed : 21068542). Involved in regulation of degradative endocytic trafficking and cytokinesis, probably in the context of PI3KC3-C2 (PubMed : 20643123). Isoform 2 acts antiapoptotic in neuronal cells; involved in maintenance of mitochondrial morphology and promotes neuronal viability (By similarity).
See full target information SH3GLB1
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