JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB101645

Recombinant Human BIT1 protein

Be the first to review this product! Submit a review

|

(1 Publication)

Recombinant Human BIT1 protein is a Human Full Length protein, in the 64 to 179 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

BIT1, PTH2, CGI-147, PTRH2, PTH 2, Bcl-2 inhibitor of transcription 1

1 Images
SDS-PAGE - Recombinant Human BIT1 protein (AB101645)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human BIT1 protein (AB101645)

ab101645 at 3 μg analysed by 15% SDS PAGE

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

Q9Y3E5

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column

Product details

This product was previously labelled as PTRH2
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMEYKMILVVRNDLKMGKGKVAAQCSHAAVSAYKQIQRRNPEMLKQWEYCGQPKVVVKAPDEETLIALLAHAKMLGLTVSLIQDAGRTQIAPGSQTVLGIGPGPADLIDKVTGHLKLY","proteinLength":"Full Length","predictedMolecularWeight":"14.9 kDa","actualMolecularWeight":null,"aminoAcidEnd":179,"aminoAcidStart":64,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q9Y3E5","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column
style
left-column

Specifications

Form

Liquid

Additional notes

ab101645 was purified using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

style
left-column

General info

Function

Peptidyl-tRNA hydrolase which releases tRNAs from the ribosome during protein synthesis (PubMed : 14660562). Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.

Sequence similarities

Belongs to the PTH2 family.

Post-translational modifications

Ubiquitinated by PRKN during mitophagy, leading to its degradation and enhancement of mitophagy. Deubiquitinated by USP30.

Subcellular localisation

Mitochondrion

style
left-column

Product protocols

style
left-column

Target data

Peptidyl-tRNA hydrolase which releases tRNAs from the ribosome during protein synthesis (PubMed : 14660562). Promotes caspase-independent apoptosis by regulating the function of two transcriptional regulators, AES and TLE1.
See full target information PTRH2
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

The Journal of neuroscience : the official journal 37:11151-11165 PubMed29038245

2017

Mitochondrial Calcium Dysregulation Contributes to Dendrite Degeneration Mediated by PD/LBD-Associated LRRK2 Mutants.

Applications

Unspecified application

Species

Unspecified reactive species

Manish Verma,Jason Callio,P Anthony Otero,Israel Sekler,Zachary P Wills,Charleen T Chu
View all publications
style
left-column
style
left-column
style
right-column

Complementary Products

Primary Antibodies

AB109291

Anti-BIT1 antibody [EPR5165]

RabMAb|Recombinant

Western blot - Anti-BIT1 antibody [EPR5165] (AB109291)

  • 0
  • 0
Proteins & Peptides

AB104121

Recombinant human HDAC1 protein

Functional Studies - Recombinant human HDAC1 protein (AB104121)

  • 0
  • 0
Proteins & Peptides

AB268350

Recombinant Human ARPP-19 protein (Tagged)

SDS-PAGE - Recombinant Human ARPP-19 protein (Tagged) (AB268350)

  • 0
  • 0
Proteins & Peptides

AB205213

Recombinant Human Nova1 protein

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com