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AB116891

Recombinant Human Blood Group Kell Antigen protein (GST tag N-Terminus)

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Recombinant Human Blood Group Kell Antigen protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 732 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

CD238, Kell blood group glycoprotein, KEL

1 Images
SDS-PAGE - Recombinant Human Blood Group Kell Antigen protein (GST tag N-Terminus) (AB116891)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Blood Group Kell Antigen protein (GST tag N-Terminus) (AB116891)

12.5% SDS-PAGE showing ab116891 at approximately 106.59kDa stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

SDS-PAGE, WB, ELISA

applications

Biologically active

No

Accession

P23276

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Recombinant Human Zinc finger protein 276 (GST tag N-Terminus)

SDS-PAGE - Recombinant Human Zinc finger protein 276 (GST tag N-Terminus) (AB164919)

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Proteins & Peptides

AB165467

Recombinant Human ZBTB46 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human ZBTB46 protein (GST tag N-Terminus) (AB165467)

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Proteins & Peptides

AB166176

Recombinant Human WDR62 protein (GST tag N-Terminus)

SDS-PAGE - Recombinant Human WDR62 protein (GST tag N-Terminus) (AB166176)

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Primary Antibodies

AB90456

Anti-Blood Group Kell Antigen antibody [MM0435-12X3]

Western blot - Anti-Blood Group Kell Antigen antibody [MM0435-12X3] (AB90456)

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  • 1
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>Recombinant protein.</p>" } } }
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Sequence info

[{"sequence":"MEGGDQSEEEPRERSQAGGMGTLWSQESTPEERLPVEGSRPWAVARRVLTAILILGLLLCFSVLLFYNFQNCGPRPCETSVCLDLRDHYLASGNTSVAPCTDFFSFACGRAKETNNSFQELATKNKNRLRRILEVQNSWHPGSGEEKAFQFYNSCMDTLAIEAAGTGPLRQVIEELGGWRISGKWTSLNFNRTLRLLMSQYGHFPFFRAYLGPHPASPHTPVIQIDQPEFDVPLKQDQEQKIYAQIFREYLTYLNQLGTLLGGDPSKVQEHSSLSISITSRLFQFLRPLEQRRAQGKLFQMVTIDQLKEMAPAIDWLSCLQATFTPMSLSPSQSLVVHDVEYLKNMSQLVEEMLLKQRDFLQSHMILGLVVTLSPALDSQFQEARRKLSQKLRELTEQPPMPARPRWMKCVEETGTFFEPTLAALFVREAFGPSTRSAAMKLFTAIRDALITRLRNLPWMNEETQNMAQDKVAQLQVEMGASEWALKPELARQEYNDIQLGSSFLQSVLSCVRSLRARIVQSFLQPHPQHRWKVSPWDVNAYYSVSDHVVVFPAGLLQPPFFHPGYPRAVNFGAAGSIMAHELLHIFYQLLLPGGCLACDNHALQEAHLCLKRHYAAFPLPSRTSFNDSLTFLENAADVGGLAIALQAYSKRLLRHHGETVLPSLDLSPQQIFFRSYAQVMCRKPSPQDSHDTHSPPHLRVHGPLSSTPAFARYFRCARGALLNPSSRCQLW","proteinLength":"Full Length","predictedMolecularWeight":"106.59 kDa","actualMolecularWeight":null,"aminoAcidEnd":732,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P23276","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

The Blood Group Kell Antigen also known as Kell protein is a type II transmembrane glycoprotein with a mass of approximately 93 kDa. It exists on the surface of red blood cells (RBCs) and has enzymatic activity specifically as a zinc endopeptidase. The primary expression site for Kell protein is the erythrocyte membrane where it functions in processing various polypeptides through its enzymatic action. There are different phenotypes like Kell positive and Kell negative and antibodies such as anti-Kell and anti-K are significant in immunohematology.
Biological function summary

The Kell protein facilitates the degradation of endothelin precursors. It belongs to the neprilysin (M13) family of zinc endopeptidases playing a critical part in modulating vasoactive peptides. Normally the Kell protein forms a complex with another protein called XK an integral membrane protein. When properly functioning this Kell-XK complex maintains RBC integrity and membrane flexibility essential for normal cellular operations and lifespan.

Pathways

The Kell antigen influences pathways involved in cardiovascular regulation and cellular movement. It has connections with the vasoregulatory pathway where it interacts with endothelins. The breakdown of endothelin precursors by Kell reduces the levels of these potent vasoconstrictors. The protein also has an indirect association with members of the endothelin-converting enzyme family which further processes these peptides into active forms.

The Kell antigen plays an important role in conditions like transfusion reactions and hemolytic disease of the newborn (HDN). In transfusion medicine incompatibility with Kell positive blood can lead to antibody production resulting in hemolytic reactions. Anti-Kell antibodies such as anti-K and anti-Kell are particularly implicated in HDN where the maternal immune response targets the Kell antigen on fetal erythrocytes. The XK protein related to Kell through the disease mechanism is also involved and mutations in XK can cause McLeod syndrome further complicating blood group antigen relationships.
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Specifications

Form

Liquid

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General info

Function

Zinc endopeptidase with endothelin-3-converting enzyme activity. Cleaves EDN1, EDN2 and EDN3, with a marked preference for EDN3.

Sequence similarities

Belongs to the peptidase M13 family.

Post-translational modifications

N-glycosylated.

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Product protocols

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Target data

Zinc endopeptidase with endothelin-3-converting enzyme activity. Cleaves EDN1, EDN2 and EDN3, with a marked preference for EDN3.
See full target information KEL
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