Recombinant Human Bmf protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for WB, SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- WB, SDS-PAGE
- Biologically active
- No
Amino acid sequence
M A S M T G G Q Q M G R G S H M E P S Q C V E E L E D D V F Q P E D G E P V T Q P G S L L S A D L F A Q S L L D C P L S R L Q L F P L T H C C G P G L R P T S Q E D K A T Q T L S P A S P S Q G V M L P C G V T E E P Q R L F Y A P A E P K S C V V A D P P L P A Q P C F E W R R E Q E R G R P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes ab85485 can be used as a WB positive control in conjunction with Anti-Bmf antibody ab9655. |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes Use 3ug/lane. |
Target data
Function
May play a role in apoptosis. Isoform 1 seems to be the main initiator.
Alternative names
Bcl-2-modifying factor, BMF
Recommended products
Recombinant Human Bmf protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for WB, SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- WB, SDS-PAGE
- Biologically active
- No
- Accession
- Q96LC9-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M A S M T G G Q Q M G R G S H M E P S Q C V E E L E D D V F Q P E D G E P V T Q P G S L L S A D L F A Q S L L D C P L S R L Q L F P L T H C C G P G L R P T S Q E D K A T Q T L S P A S P S Q G V M L P C G V T E E P Q R L F Y A P A E P K S C V V A D P P L P A Q P C F E W R R E Q E R G R P
- Accession
- Q96LC9
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab85485 was purified by using conventional chromatography.
General info
- Function
May play a role in apoptosis. Isoform 1 seems to be the main initiator.
- Sequence similarities
Belongs to the Bcl-2 family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method )
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bmf also known as Bcl-2 modifying factor is important in apoptosis regulation a process of programmed cell death. It acts as a pro-apoptotic protein from the Bcl-2 family. The Bmf protein has a molecular weight of approximately 21 kDa. It is mainly expressed in various epithelial tissues and abundant in hematopoietic cells. Bmf can interact with the dynein light chain at the cytoskeleton which affects its localization and function.
- Biological function summary
The protein exerts its function by promoting apoptosis. Bmf binds directly to pro-survival Bcl-2 family members such as Bcl-X_L and Bcl-2 displacing pro-apoptotic proteins like Bax or Bak. This action facilitates the release of cytochrome c from mitochondria triggering caspase activation and cell death. Bmf can function independently but also works within larger complexes affecting processes related to cellular homeostasis.
- Pathways
Bmf operates prominently within the intrinsic apoptotic pathway where it modulates mitochondrial outer membrane permeability. Its action is closely associated with Bax and Bak proteins facilitating their oligomerization. Additionally Bmf integrates into stress-induced signaling pathways responding to signals like oxidative stress or deprivation of survival factors. Through these connections Bmf influences cell fate and stress responses often cross-talking with the JNK signaling pathway.
- Associated diseases and disorders
Bmf has implications in several types of cancer and neurodegenerative conditions. Overexpression or dysregulation of Bmf can contribute to tumor development due to impaired apoptosis. Specific cancers like lymphomas show altered Bmf levels linking it to pathways with proteins such as Bcl-2 and Bax. In neurodegenerative diseases changes in Bmf expression can impact neuronal survival with implications for conditions like Alzheimer's where its interaction with other Bcl-2 family proteins affects cell viability.
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3 product images
Western blot - Recombinant Human Bmf protein (ab85485)
All lanes: Western blot - Anti-Bmf antibody (Anti-Bmf antibody ab9655) at 2 µg/mL
All lanes: Western blot - Recombinant Human Bmf protein (ab85485) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Exposure time: 8min
SDS-PAGE - Recombinant Human Bmf protein (ab85485)
15% SDS-PAGE analysis of 3μg ab85485
Western blot - Recombinant Human Bmf protein (ab85485)
All lanes: Western blot - Anti-Bmf antibody (Anti-Bmf antibody ab9655) at 2 µg/mL
All lanes: Western blot - Recombinant Human Bmf protein (ab85485) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Exposure time: 8min
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