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BiochemicalsProteins and Peptides
Proteins and peptidesAnti-Ly6g antibody [1A8] - mouse IgG2c (Chimeric)
Low endotoxin, Azide free.
Our first-to-market chimera with mouse IgG2c backbone, this functional antibody specifically depletes neutrophils in vivo for up to 72h.
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Recombinant Human Bmf protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for WB, SDS-PAGE.
>90% SDS-PAGE
Escherichia coli
Tag free
WB, SDS-PAGE
No
M A S M T G G Q Q M G R G S H M E P S Q C V E E L E D D V F Q P E D G E P V T Q P G S L L S A D L F A Q S L L D C P L S R L Q L F P L T H C C G P G L R P T S Q E D K A T Q T L S P A S P S Q G V M L P C G V T E E P Q R L F Y A P A E P K S C V V A D P P L P A Q P C F E W R R E Q E R G R P
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes Ab85485 can be used as a WB positive control in conjunction with ab9655. |
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes Use 3ug/lane. |
May play a role in apoptosis. Isoform 1 seems to be the main initiator.
Bcl-2-modifying factor, BMF
Recombinant Human Bmf protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for WB, SDS-PAGE.
Bcl-2-modifying factor, BMF
>90% SDS-PAGE
Escherichia coli
Tag free
WB, SDS-PAGE
No
No
Human
pH: 7.5
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
M A S M T G G Q Q M G R G S H M E P S Q C V E E L E D D V F Q P E D G E P V T Q P G S L L S A D L F A Q S L L D C P L S R L Q L F P L T H C C G P G L R P T S Q E D K A T Q T L S P A S P S Q G V M L P C G V T E E P Q R L F Y A P A E P K S C V V A D P P L P A Q P C F E W R R E Q E R G R P
Full Length
Recombinant
Liquid
ab85485 was purified by using conventional chromatography.
May play a role in apoptosis. Isoform 1 seems to be the main initiator.
Belongs to the Bcl-2 family.
Blue Ice
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method )
The protein exerts its function by promoting apoptosis. Bmf binds directly to pro-survival Bcl-2 family members such as Bcl-X_L and Bcl-2 displacing pro-apoptotic proteins like Bax or Bak. This action facilitates the release of cytochrome c from mitochondria triggering caspase activation and cell death. Bmf can function independently but also works within larger complexes affecting processes related to cellular homeostasis.
Bmf also known as Bcl-2 modifying factor is important in apoptosis regulation a process of programmed cell death. It acts as a pro-apoptotic protein from the Bcl-2 family. The Bmf protein has a molecular weight of approximately 21 kDa. It is mainly expressed in various epithelial tissues and abundant in hematopoietic cells. Bmf can interact with the dynein light chain at the cytoskeleton which affects its localization and function.
Bmf operates prominently within the intrinsic apoptotic pathway where it modulates mitochondrial outer membrane permeability. Its action is closely associated with Bax and Bak proteins facilitating their oligomerization. Additionally Bmf integrates into stress-induced signaling pathways responding to signals like oxidative stress or deprivation of survival factors. Through these connections Bmf influences cell fate and stress responses often cross-talking with the JNK signaling pathway.
Bmf has implications in several types of cancer and neurodegenerative conditions. Overexpression or dysregulation of Bmf can contribute to tumor development due to impaired apoptosis. Specific cancers like lymphomas show altered Bmf levels linking it to pathways with proteins such as Bcl-2 and Bax. In neurodegenerative diseases changes in Bmf expression can impact neuronal survival with implications for conditions like Alzheimer's where its interaction with other Bcl-2 family proteins affects cell viability.
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Full details and terms and conditions can be found here:
Terms & Conditions.
All lanes: Western blot - Anti-Bmf antibody (AB9655) at 2 µg/mL
All lanes: Western blot - Recombinant Human Bmf protein (AB85485) at 0.1 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Exposure time: 8min
15% SDS-PAGE analysis of 3μg ab85485
All lanes: Western blot - Anti-Bmf antibody (AB9655) at 2 µg/mL
All lanes: Western blot - Recombinant Human Bmf protein (AB85485) at 0.1 µg
All lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (AB97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 21 kDa
Exposure time: 8min
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