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AB114169

Recombinant Human Bmi1 protein

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Recombinant Human Bmi1 protein is a Human Full Length protein, in the 1 to 326 aa range, expressed in Wheat germ, suitable for SDS-PAGE, ELISA, WB.

View Alternative Names

PCGF4, RNF51, BMI1, Polycomb complex protein BMI-1, Polycomb group RING finger protein 4, RING finger protein 51

2 Images
Western blot - Recombinant Human Bmi1 protein (AB114169)
  • WB

Unknown

Western blot - Recombinant Human Bmi1 protein (AB114169)

ab63767 recognizes the full length tagged recombinant Bmi1 protein (ab114169) which has an expected molecular weight of 62 kDa.

All lanes:

Anti-Bmi1 antibody (<a href='/en-us/products/unavailable/bmi1-antibody-ab63767'>ab63767</a>) at 2 µg/mL

All lanes:

Western blot - Recombinant Human Bmi1 protein (ab114169) at 0.1 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 30s

SDS-PAGE - Recombinant Human Bmi1 protein (AB114169)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Bmi1 protein (AB114169)

ab114169 on a 12.5% SDS-PAGE Stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

Tag free

Applications

WB, SDS-PAGE, ELISA

applications

Biologically active

No

Accession

P35226

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.3% Glutathione

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab114169 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/bmi1-antibody-ab63767'>ab63767</a>.</p>" } } }
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Sequence info

[{"sequence":"MHRTTRIKITELNPHLMCVLCGGYFIDATTIIECLHSFCKTCIVRYLETSKYCPICDVQVHKTRPLLNIRSDKTLQDIVYKLVPGLFKNEMKRRRDFYAAHPSADAANGSNEDRGEVADEDKRIITDDEIISLSIEFFDQNRLDRKVNKDKEKSKEEVNDKRYLRCPAAMTVMHLRKFLRSKMDIPNTFQIDVMYEEEPLKDYYTLMDIAYIYTWRRNGPLPLKYRVRPTCKRMKISHQRDGLTNAGELESDSGSDKANSPAGGIPSTSSCLPSPSTPVQSPHPQFPHISSTMNGTSNSPSGNHQSSFANRPRKASVNGSSATSSG","proteinLength":"Full Length","predictedMolecularWeight":"61.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":326,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"P35226","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bmi1 also known as B-cell-specific Moloney murine leukemia virus integration site 1 is a member of the Polycomb group of proteins. It has an approximate molecular mass of 37 kDa. Bmi1 is expressed in many tissues including the brain bone marrow and hematopoietic cells. It functions as a transcriptional repressor. Bmi1 plays an important role in chromatin remodeling influencing gene silencing and regulation of developmental processes. Bmi1 ELISA assays are commonly used for quantifying its levels in various research applications.
Biological function summary

The functions of Bmi1 extend into maintaining stem cell renewal and differentiation. It acts as part of the Polycomb repressive complex 1 (PRC1) partnering with other proteins to regulate gene expression by altering chromatin structure. Bmi1 protein regulates cell proliferation and inhibits senescence by repressing the INK4a/ARF locus which encodes tumor suppressor proteins like p16INK4a and p14ARF.

Pathways

Bmi1 plays significant roles in processes such as the DNA damage response and the self-renewal of hematopoietic stem cells. It is involved in the modulation of the Wnt and Notch signaling pathways. Bmi1 protein interacts closely with proteins such as CBX2 and RNF2 within these pathways affecting the transcriptional landscape that governs cell fate decisions.

Bmi1 overexpression links to cancer particularly breast cancer and leukemia. In MCF7 breast cancer cells alterations in Bmi1 activity contribute to oncogenesis and tumor progression. Its dysregulation can connect to Myc a well-known oncogenic protein. Additionally Bmi1's influence in Cas9-based gene editing models highlights its potential in cancer therapy research. Bmi1 has also been implicated in neurodegenerative disorders with connections to proteins like p21CIP1 affecting neuronal survival and aging.
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Specifications

Form

Liquid

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General info

Function

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 15386022, PubMed : 16359901, PubMed : 16714294, PubMed : 21772249, PubMed : 25355358, PubMed : 26151332, PubMed : 27827373). The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed : 21772249, PubMed : 25355358). In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed : 15386022, PubMed : 21772249, PubMed : 26151332).

Post-translational modifications

Monoubiquitinated (By similarity). May be polyubiquitinated; which does not lead to proteasomal degradation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones; it mediates monoubiquitination of histone H2A 'Lys-119', rendering chromatin heritably changed in its expressibility (PubMed : 15386022, PubMed : 16359901, PubMed : 16714294, PubMed : 21772249, PubMed : 25355358, PubMed : 26151332, PubMed : 27827373). The complex composed of RNF2, UB2D3 and BMI1 binds nucleosomes, and has activity only with nucleosomal histone H2A (PubMed : 21772249, PubMed : 25355358). In the PRC1-like complex, regulates the E3 ubiquitin-protein ligase activity of RNF2/RING2 (PubMed : 15386022, PubMed : 21772249, PubMed : 26151332).
See full target information BMI1
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