Recombinant human BMP2 protein (Active) is a Human Full Length protein, in the 283 to 396 aa range, expressed in Escherichia coli, with >98% purity and suitable for SDS-PAGE, HPLC, CellAct.
>98% SDS-PAGE
Escherichia coli
Tag free
SDS-PAGE, HPLC, CellAct
Yes
M Q A K H K Q R K R L K S S C K R H P L Y V D F S D V G W N D W I V A P P G Y H A F Y C H G E C P F P L A D H L N S T N H A I V Q T L V N S V N S K I P K A C C V P T E L S A I S M L Y L D E N E K V V L K N Y Q D M V V E G C G C R
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application HPLC | Reactivity Reacts | Dilution info - | Notes - |
Application CellAct | Reactivity Reacts | Dilution info - | Notes The ED50 for inducing alkaline phosphatase production by ATDC-5 cells is < 1µg/ml. |
Select an associated product type
Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis (PubMed:18436533, PubMed:24362451, PubMed:31019025). Induces cartilage and bone formation (PubMed:3201241). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 (PubMed:15064755, PubMed:17295905, PubMed:18436533). Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A (PubMed:7791754). In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Also acts to promote expression of HAMP, via the interaction with its receptor BMPR1A/ALK3 (PubMed:31800957). Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation (PubMed:16771708, PubMed:20851880). Also stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation (PubMed:24362451). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity).
BMP2A, BMP2A, BMP2, Bone morphogenetic protein 2, BMP-2, Bone morphogenetic protein 2A, BMP-2A
Recombinant human BMP2 protein (Active) is a Human Full Length protein, in the 283 to 396 aa range, expressed in Escherichia coli, with >98% purity and suitable for SDS-PAGE, HPLC, CellAct.
>98% SDS-PAGE
Escherichia coli
Tag free
SDS-PAGE, HPLC, CellAct
Yes
Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells.
The expected ED50 for this effect is 0.5-1.0 μg/ml.
No
Human
Centrifuge the vial prior to opening. Reconstitute in water containing BSA (50ug of BSA per 1ug of protein) to a concentration of 0.1-1.0 mg/ml. This solution can then be diluted into other aqueous buffers and stored at 4°C for 1 week or -20°C for future use.
M Q A K H K Q R K R L K S S C K R H P L Y V D F S D V G W N D W I V A P P G Y H A F Y C H G E C P F P L A D H L N S T N H A I V Q T L V N S V N S K I P K A C C V P T E L S A I S M L Y L D E N E K V V L K N Y Q D M V V E G C G C R
Full Length
26 kDa
283 to 396
Recombinant
Lyophilized
>98% by HPLC analyses.
Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis (PubMed:18436533, PubMed:24362451, PubMed:31019025). Induces cartilage and bone formation (PubMed:3201241). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 (PubMed:15064755, PubMed:17295905, PubMed:18436533). Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A (PubMed:7791754). In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Also acts to promote expression of HAMP, via the interaction with its receptor BMPR1A/ALK3 (PubMed:31800957). Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation (PubMed:16771708, PubMed:20851880). Also stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation (PubMed:24362451). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity).
Belongs to the TGF-beta family.
Blue Ice
1-2 weeks
+4°C
-20°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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