Recombinant human BMP2 protein (Fc Chimera) is a Human Fragment protein, in the 289 to 396 aa range, expressed in CHO, with >=98% purity, < 0.06 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
>=98% SDS-PAGE
< 0.06 EU/µg
CHO cells
Tag free
SDS-PAGE, FuncS
Yes
R K R L K S S C K R H P L Y V D F S D V G W N D W I V A P P G Y H A F Y C H G E C P F P L A D H L N S T N H A I V Q T L V N S V N S K I P K A C C V P T E L S A I S M L Y L D E N E K V V L K N Y Q D M V V E G C G C R
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis (PubMed:18436533, PubMed:24362451, PubMed:31019025). Induces cartilage and bone formation (PubMed:3201241). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 (PubMed:15064755, PubMed:17295905, PubMed:18436533). Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A (PubMed:7791754). In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Also acts to promote expression of HAMP, via the interaction with its receptor BMPR1A/ALK3 (PubMed:31800957). Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation (PubMed:16771708, PubMed:20851880). Also stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation (PubMed:24362451). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity).
BMP2A, BMP2A, BMP2, Bone morphogenetic protein 2, BMP-2, Bone morphogenetic protein 2A, BMP-2A
Recombinant human BMP2 protein (Fc Chimera) is a Human Fragment protein, in the 289 to 396 aa range, expressed in CHO, with >=98% purity, < 0.06 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
>=98% SDS-PAGE
< 0.06 EU/µg
CHO cells
Tag free
SDS-PAGE, FuncS
Yes
Shows the biological function of the BMP-2 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain in vivo. Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 2.0 -3.5μg/ml.
No
Yes
Human
Reconstitute in 50µl sterile water. Add 1X PBS to the desired protein concentration.
Constituents: 100% PBS
R K R L K S S C K R H P L Y V D F S D V G W N D W I V A P P G Y H A F Y C H G E C P F P L A D H L N S T N H A I V Q T L V N S V N S K I P K A C C V P T E L S A I S M L Y L D E N E K V V L K N Y Q D M V V E G C G C R
Fragment
289 to 396
Recombinant
Lyophilized
Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis (PubMed:18436533, PubMed:24362451, PubMed:31019025). Induces cartilage and bone formation (PubMed:3201241). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 (PubMed:15064755, PubMed:17295905, PubMed:18436533). Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A (PubMed:7791754). In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Also acts to promote expression of HAMP, via the interaction with its receptor BMPR1A/ALK3 (PubMed:31800957). Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation (PubMed:16771708, PubMed:20851880). Also stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation (PubMed:24362451). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity).
Belongs to the TGF-beta family.
Blue Ice
1-2 weeks
+4°C
-20°C
Avoid freeze / thaw cycle, Stable for 12 months at -20°C
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).
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