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Recombinant human BMP2 protein (Fc Chimera) is a Human Fragment protein, in the 289 to 396 aa range, expressed in CHO, with >=98% purity, < 0.06 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.

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Key facts

Purity

>=98% SDS-PAGE

Endotoxin level

< 0.06 EU/µg

Expression system

CHO cells

Tags

Tag free

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Amino acid sequence

R K R L K S S C K R H P L Y V D F S D V G W N D W I V A P P G Y H A F Y C H G E C P F P L A D H L N S T N H A I V Q T L V N S V N S K I P K A C C V P T E L S A I S M L Y L D E N E K V V L K N Y Q D M V V E G C G C R

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

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Target data

Function

Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis (PubMed:18436533, PubMed:24362451, PubMed:31019025). Induces cartilage and bone formation (PubMed:3201241). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 (PubMed:15064755, PubMed:17295905, PubMed:18436533). Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A (PubMed:7791754). In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Also acts to promote expression of HAMP, via the interaction with its receptor BMPR1A/ALK3 (PubMed:31800957). Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation (PubMed:16771708, PubMed:20851880). Also stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation (PubMed:24362451). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity).

Alternative names

Recommended products

Recombinant human BMP2 protein (Fc Chimera) is a Human Fragment protein, in the 289 to 396 aa range, expressed in CHO, with >=98% purity, < 0.06 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>=98% SDS-PAGE

Endotoxin level

< 0.06 EU/µg

Expression system

CHO cells

Applications

SDS-PAGE, FuncS

Biological activity

Shows the biological function of the BMP-2 moiety and exerts a prolonged circulating half-life caused by the modified Fc domain in vivo. Determined by its ability to induce alkaline phosphatase production by ATDC-5 cells. The expected ED50 for this effect is 2.0 -3.5μg/ml.

Accession
P12643-1
Animal free

No

Carrier free

Yes

Species

Human

Reconstitution

Reconstitute in 50µl sterile water. Add 1X PBS to the desired protein concentration.

Concentration
Loading...
Storage buffer

Constituents: 100% PBS

Sequence info

Amino acid sequence

R K R L K S S C K R H P L Y V D F S D V G W N D W I V A P P G Y H A F Y C H G E C P F P L A D H L N S T N H A I V Q T L V N S V N S K I P K A C C V P T E L S A I S M L Y L D E N E K V V L K N Y Q D M V V E G C G C R

Accession

P12643

Protein length

Fragment

Amino acids

289 to 396

Nature

Recombinant

Specifications

Form

Lyophilized

General info

Function

Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cardiogenesis, neurogenesis, and osteogenesis (PubMed:18436533, PubMed:24362451, PubMed:31019025). Induces cartilage and bone formation (PubMed:3201241). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 (PubMed:15064755, PubMed:17295905, PubMed:18436533). Once all three components are bound together in a complex at the cell surface, BMPR2 phosphorylates and activates BMPR1A (PubMed:7791754). In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes. Also acts to promote expression of HAMP, via the interaction with its receptor BMPR1A/ALK3 (PubMed:31800957). Can also signal through non-canonical pathways such as ERK/MAP kinase signaling cascade that regulates osteoblast differentiation (PubMed:16771708, PubMed:20851880). Also stimulates the differentiation of myoblasts into osteoblasts via the EIF2AK3-EIF2A-ATF4 pathway by stimulating EIF2A phosphorylation which leads to increased expression of ATF4 which plays a central role in osteoblast differentiation (PubMed:24362451). Acts as a positive regulator of odontoblast differentiation during mesenchymal tooth germ formation, expression is repressed during the bell stage by MSX1-mediated inhibition of CTNNB1 signaling (By similarity).

Sequence similarities

Belongs to the TGF-beta family.

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage duration

1-2 weeks

Appropriate short-term storage conditions

+4°C

Appropriate long-term storage conditions

-20°C

Storage information

Avoid freeze / thaw cycle, Stable for 12 months at -20°C

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Non-lytic: Acts as a long lasting fusion protein which only binds to the receptor. Mutations to the complement (C1q) and FcgR I binding sites of the IgGs Fc fragment render the fusion proteins incapable of antibody directed cytotoxicity (ADCC) and complement directed cytotoxicity (CDC).

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