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AB87627

Recombinant Human BMP5 protein

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(0 Publication)

Recombinant Human BMP5 protein is a Human Full Length protein, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

Bone morphogenetic protein 5, BMP-5, BMP5

2 Images
Western blot - Recombinant Human BMP5 protein (AB87627)
  • WB

Unknown

Western blot - Recombinant Human BMP5 protein (AB87627)

All lanes:

Anti-BMP5 antibody (<a href='/en-us/products/unavailable/bmp5-antibody-ab93852'>ab93852</a>) at 1 µg/mL

Lane 1:

Western blot - Recombinant Human BMP5 protein (ab87627) at 0.1 µg

Lane 2:

Western blot - Recombinant Human BMP5 protein (ab87627) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

true

Exposure time: 1min

SDS-PAGE - Recombinant Human BMP5 protein (AB87627)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human BMP5 protein (AB87627)

15% SDS-PAGE showing ab87627 at approximately 15.7kDa (3μg).

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

WB, SDS-PAGE

applications

Biologically active

No

Accession

P22003

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 3.5 Constituents: 10% Glycerol (glycerin, glycerine), 0.294% Sodium citrate

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab87627 can be used as a WB positive control in conjunction with <a href='/en-us/products/unavailable/bmp5-antibody-ab93852'>ab93852</a>.</p>" } } }
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Sequence info

[{"sequence":"MAANKRKNQNRNKSSSHQDSSRMSSVGDYNTSEQKQACKKHELYVSFRDLGWQDWIIAPEGYAAFYCDGECSFPLNAHMNATNHAIVQTLVHLMFPDHVPKPCCAPTKLNAISVLYFDDSSNVILKKYRNMVVRSCGCH","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P22003","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

Additional notes

ab87627 is purified by conventional chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.Endotoxin: < 1.0 EU per 1 μg of protein (determined by LAL method).

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General info

Function

Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cartilage and bone formation or neurogenesis (PubMed : 11580864, PubMed : 29321139). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 (PubMed : 11580864). In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes (PubMed : 11580864, PubMed : 29321139). Can also signal through non-canonical pathway such as MAPK p38 signaling cascade to promote chondrogenic differentiation (PubMed : 20402566). Promotes the expression of HAMP, this is repressed by its interaction with ERFE (PubMed : 30097509).

Sequence similarities

Belongs to the TGF-beta family.

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Product protocols

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Target data

Growth factor of the TGF-beta superfamily that plays essential roles in many developmental processes, including cartilage and bone formation or neurogenesis (PubMed : 11580864, PubMed : 29321139). Initiates the canonical BMP signaling cascade by associating with type I receptor BMPR1A and type II receptor BMPR2 (PubMed : 11580864). In turn, BMPR1A propagates signal by phosphorylating SMAD1/5/8 that travel to the nucleus and act as activators and repressors of transcription of target genes (PubMed : 11580864, PubMed : 29321139). Can also signal through non-canonical pathway such as MAPK p38 signaling cascade to promote chondrogenic differentiation (PubMed : 20402566). Promotes the expression of HAMP, this is repressed by its interaction with ERFE (PubMed : 30097509).
See full target information Bone morphogenetic protein 5
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Product promise

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