Recombinant human BMPR1B protein is a Human Fragment protein, in the 149 to 502 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, WB, FuncS.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP7/OP-1 and GDF5. Positively regulates chondrocyte differentiation through GDF5 interaction.
Alternative names
CDw293, Bone morphogenetic protein receptor type-1B, BMP type-1B receptor, BMPR-1B, BMPR1B
Recommended products
Recombinant human BMPR1B protein is a Human Fragment protein, in the 149 to 502 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, WB, FuncS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- Tag free
- Applications
- SDS-PAGE, WB, FuncS
- Biologically active
- Yes
- Biological activity
- The Specific activity of ab107949 was determined to be 23 nmol/min/mg.
- Accession
- O00238-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.307% Glutathione, 0.00385% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00292% EDTA, 0.00174% PMSF
Sequence info
Amino acid sequence
- Accession
- O00238
- Protein length
- Fragment
- Predicted molecular weight
- 68 kDa
- Amino acids
- 149 to 502
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
Purity was determined to be >85% by densitometry. Affinity purified.
General info
- Function
On ligand binding, forms a receptor complex consisting of two type II and two type I transmembrane serine/threonine kinases. Type II receptors phosphorylate and activate type I receptors which autophosphorylate, then bind and activate SMAD transcriptional regulators. Receptor for BMP7/OP-1 and GDF5. Positively regulates chondrocyte differentiation through GDF5 interaction.
- Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. TGFB receptor subfamily.
- Post-translational modifications
Autophosphorylated.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
Smad3 peptide ab204884 (Smad3 peptide) can be utilized as a substrate for assessing kinase activity
Product promise
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4 product images
Functional Studies - Recombinant human BMPR1B protein (ab107949)
The specific activity of BMPR1B (ab107949) was determined to be 19 nmol/min/mg as per activity assay protocol
SDS-PAGE - Recombinant human BMPR1B protein (ab107949)
SDS PAGE analysis of ab107949
Functional Studies - Recombinant human BMPR1B protein (ab107949)
Kinase Assay demonstrating specific activity of ab107949.
SDS-PAGE - Recombinant human BMPR1B protein (ab107949)
SDS-PAGE showing ab107949 at approximately 68kDa.
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Product protocols
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