Recombinant Human Bone marrow stromal cell antigen 1 protein (His tag) is a Human Fragment protein, in the 33 to 293 aa range, expressed in Baculovirus infected insect, with >95% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 0.1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
R W R G E G T S A H L R D I F L G R C A E Y R A L L S P E Q R N K N C T A I W E A F K V A L D K D P C S V L P S D Y D L F I N L S R H S I P R D K S L F W E N S H L L V N S F A D N T R R F M P L S D V L Y G R V A D F L S W C R Q K N D S G L D Y Q S C P T S E D C E N N P V D S F W K R A S I Q Y S K D S S G V I H V M L N G S E P T G A Y P I K G F F A D Y E I P N L Q K E K I T R I E I W V M H E I G G P N V E S C G E G S M K V L E K R L K D M G F Q Y S C I N D Y R P V K L L Q C V D H S T H P D C A L K S A A A A T Q R K A H H H H H H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Catalyzes both the synthesis of cyclic ADP-beta-D-ribose (cADPR) from NAD(+), and its hydrolysis to ADP-D-ribose (ADPR) (PubMed:7805847). Cyclic ADPR is known to serve as an endogenous second messenger that elicits calcium release from intracellular stores, and thus regulates the mobilization of intracellular calcium (Probable). May be involved in pre-B-cell growth (Probable).
Alternative names
CD157, ADP-ribosyl cyclase/cyclic ADP-ribose hydrolase 2, ADP-ribosyl cyclase 2, Bone marrow stromal cell antigen 1, Cyclic ADP-ribose hydrolase 2, BST-1, cADPR hydrolase 2, BST1
Recommended products
Recombinant Human Bone marrow stromal cell antigen 1 protein (His tag) is a Human Fragment protein, in the 33 to 293 aa range, expressed in Baculovirus infected insect, with >95% purity, < 0.1 EU/µg endotoxin level and suitable for SDS-PAGE.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 0.1 EU/µg
- Expression system
- Baculovirus infected insect cells
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- Q10588-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: PBS, 10% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- R W R G E G T S A H L R D I F L G R C A E Y R A L L S P E Q R N K N C T A I W E A F K V A L D K D P C S V L P S D Y D L F I N L S R H S I P R D K S L F W E N S H L L V N S F A D N T R R F M P L S D V L Y G R V A D F L S W C R Q K N D S G L D Y Q S C P T S E D C E N N P V D S F W K R A S I Q Y S K D S S G V I H V M L N G S E P T G A Y P I K G F F A D Y E I P N L Q K E K I T R I E I W V M H E I G G P N V E S C G E G S M K V L E K R L K D M G F Q Y S C I N D Y R P V K L L Q C V D H S T H P D C A L K S A A A A T Q R K A H H H H H H
- Accession
- Q10588
- Protein length
- Fragment
- Predicted molecular weight
- 30.5 kDa
- Amino acids
- 33 to 293
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified
General info
- Function
Catalyzes both the synthesis of cyclic ADP-beta-D-ribose (cADPR) from NAD(+), and its hydrolysis to ADP-D-ribose (ADPR) (PubMed:7805847). Cyclic ADPR is known to serve as an endogenous second messenger that elicits calcium release from intracellular stores, and thus regulates the mobilization of intracellular calcium (Probable). May be involved in pre-B-cell growth (Probable).
- Sequence similarities
Belongs to the ADP-ribosyl cyclase family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Bone marrow stromal cell antigen 1 (BST-1) also known as CD157 is a glycosylphosphatidylinositol-anchored protein with a molecular weight of approximately 38-42 kDa. You find BST-1 expression on the surface of various cell types especially within bone marrow stromal cells and cells of the immune system such as monocytes and macrophages. This protein functions as an ecto-enzyme with ADP-ribosyl cyclase activity and is part of the NADase enzyme family. Its mechanical role involves metabolizing extracellular NAD+ influencing cellular calcium signaling and energy metabolism.
- Biological function summary
BST-1 contributes to the regulation of cellular interactions and immune responses. It participates in the organization of leukocyte trafficking and the formation of signal transduction cascades. BST-1 operates as a single protein but interacts with other membrane proteins to coordinate cellular communication. You can observe its function in processes like cell adhesion and migration impacting the immune surveillance and response systems.
- Pathways
BST-1 plays a role in immune signaling and calcium-signaling pathways. In the immune system pathway it associates with molecules such as integrins to modulate leukocyte adhesion and transmigration. The calcium-signaling pathway is another key area where BST-1 interacts with intracellular proteins influencing various cellular targets and affecting metabolic processes critical for cell survival and proliferation.
- Associated diseases and disorders
Scientists have linked BST-1 to various conditions such as leukemia and chronic inflammation. Its expression levels often change in these diseases emphasizing its potential role in their progression. Researchers also explore the connection between BST-1 and other proteins like CD38 in the context of leukemia where both proteins might participate in cancer cell proliferation and survival. Understanding BST-1 in disease contexts provides insights into disease mechanisms and potential therapeutic strategies.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human Bone marrow stromal cell antigen 1 protein (His tag) (ab235881)
15% SDS-PAGE analysis of 3 μg ab235881.
28-40 kDa (Under reducing conditions)
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com