Recombinant human BRAF (mutated L597V) protein is a Human Fragment protein, in the 381 to 766 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >70% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
D L I R D Q G F R G D G G S T T G L S A T P P A S L P G S L T N V K A L Q K S P G P Q R E R K S S S S S E D R N R M K T L G R R D S S D D W E I P D G Q I T V G Q R I G S G S F G T V Y K G K W H G D V A V K M L N V T A P T P Q Q L Q A F K N E V G V L R K T R H V N I L L F M G Y S T K P Q L A I V T Q W C E G S S L Y H H L H I I E T K F E M I K L I D I A R Q T A Q G M D Y L H A K S I I H R D L K S N N I F L H E D L T V K I G D F G V A T V K S R W S G S H Q F E Q L S G S I L W M A P E V I R M Q D K N P Y S F Q S D V Y A F G I V L Y E L M T G Q L P Y S N I N N R D Q I I F M V G R G Y L S P D L S K V R S N C P K A M K R L M A E C L K K K R D E R P L F P Q I L A S I E L L A R S L P K I H R S A S E P S L N R A G F Q T E D F S L Y A C A S P K T P I Q A G G Y G A F P V H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
4 products for Alternative Product
Target data
Function
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). Phosphorylates PFKFB2 (PubMed:36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).
Alternative names
BRAF1, RAFB1, BRAF, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1
Recommended products
Recombinant human BRAF (mutated L597V) protein is a Human Fragment protein, in the 381 to 766 aa range, expressed in Baculovirus infected Sf9, with >70% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >70% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab204186 was determined to be 2000 nmol/min/mg.
- Accession
- P15056-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- D L I R D Q G F R G D G G S T T G L S A T P P A S L P G S L T N V K A L Q K S P G P Q R E R K S S S S S E D R N R M K T L G R R D S S D D W E I P D G Q I T V G Q R I G S G S F G T V Y K G K W H G D V A V K M L N V T A P T P Q Q L Q A F K N E V G V L R K T R H V N I L L F M G Y S T K P Q L A I V T Q W C E G S S L Y H H L H I I E T K F E M I K L I D I A R Q T A Q G M D Y L H A K S I I H R D L K S N N I F L H E D L T V K I G D F G V A T V K S R W S G S H Q F E Q L S G S I L W M A P E V I R M Q D K N P Y S F Q S D V Y A F G I V L Y E L M T G Q L P Y S N I N N R D Q I I F M V G R G Y L S P D L S K V R S N C P K A M K R L M A E C L K K K R D E R P L F P Q I L A S I E L L A R S L P K I H R S A S E P S L N R A G F Q T E D F S L Y A C A S P K T P I Q A G G Y G A F P V H
- Accession
- P15056
- Protein length
- Fragment
- Predicted molecular weight
- 69 kDa
- Amino acids
- 381 to 766
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). Phosphorylates PFKFB2 (PubMed:36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).
- Sequence similarities
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
- Post-translational modifications
Phosphorylation at Ser-365 by SGK1 inhibits its activity (Ref.8, PubMed:11410590). Phosphorylation at Thr-753 by MAPK1 (PubMed:19710016). Dephosphorylation of Ser-365 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC); this relieves inactivation and stimulates kinase activity (PubMed:35768504, PubMed:35830882).
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
2 product images
SDS-PAGE - Recombinant human BRAF (mutated L597V) protein (ab204186)
SDS-PAGE analysis of ab204186.
Functional Studies - Recombinant human BRAF (mutated L597V) protein (ab204186)
Kinase Assay demonstrating specific activity of ab204186.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com