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Recombinant human BRAF protein is a Human Fragment protein, in the 381 to 766 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.

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Images

SDS-PAGE - Recombinant human BRAF protein (AB204208), expandable thumbnail
  • Functional Studies - Recombinant human BRAF protein (AB204208), expandable thumbnail

Publications

Key facts

Purity

>90% Densitometry

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Amino acid sequence

D L I R D Q G F R G D G G S T T G L S A T P P A S L P G S L T N V K A L Q K S P G P Q R E R K S S S S S E D R N R M K T L G R R D S S D D W E I P D G Q I T V G Q R I G S G S F G T V Y K G K W H G D V A V K M L N V T A P T P Q Q L Q A F K N E V G V L R K T R H V N I L L F M G Y S T K P Q L A I V T Q W C E G S S L Y H H L H I I E T K F E M I K L I D I A R Q T A Q G M D Y L H A K S I I H R D L K S N N I F L H E D L T V K I G D F G L A T V K S R W S G S H Q F E Q L S G S I L W M A P E V I R M Q D K N P Y S F Q S D V Y A F G I V L Y E L M T G Q L P Y S N I N N R D Q I I F M V G R G Y L S P D L S K V R S N C P K A M K R L M A E C L K K K R D E R P L F P Q I L A S I E L L A R S L P K I H R S A S E P S L N R A G F Q T E D F S L Y A C A S P K T P I Q A G G Y G A F P V H

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

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Target data

Function

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). Phosphorylates PFKFB2 (PubMed:36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).

Alternative names

Recommended products

Recombinant human BRAF protein is a Human Fragment protein, in the 381 to 766 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>90% Densitometry

Expression system

Baculovirus infected Sf9 cells

Applications

SDS-PAGE, FuncS

Biological activity

The specific activity of ab204208 was determined to be 2,300 nmol/min/mg.

Accession
P15056-1
Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF

Sequence info

Amino acid sequence

D L I R D Q G F R G D G G S T T G L S A T P P A S L P G S L T N V K A L Q K S P G P Q R E R K S S S S S E D R N R M K T L G R R D S S D D W E I P D G Q I T V G Q R I G S G S F G T V Y K G K W H G D V A V K M L N V T A P T P Q Q L Q A F K N E V G V L R K T R H V N I L L F M G Y S T K P Q L A I V T Q W C E G S S L Y H H L H I I E T K F E M I K L I D I A R Q T A Q G M D Y L H A K S I I H R D L K S N N I F L H E D L T V K I G D F G L A T V K S R W S G S H Q F E Q L S G S I L W M A P E V I R M Q D K N P Y S F Q S D V Y A F G I V L Y E L M T G Q L P Y S N I N N R D Q I I F M V G R G Y L S P D L S K V R S N C P K A M K R L M A E C L K K K R D E R P L F P Q I L A S I E L L A R S L P K I H R S A S E P S L N R A G F Q T E D F S L Y A C A S P K T P I Q A G G Y G A F P V H

Accession

P15056

Protein length

Fragment

Predicted molecular weight

69 kDa

Amino acids

381 to 766

Nature

Recombinant

Tags

GST tag N-Terminus

Specifications

Form

Liquid

Additional notes

Purity is lot specific. Please contact our technical Support team for details. Affinity purified.

General info

Function

Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). Phosphorylates PFKFB2 (PubMed:36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).

Sequence similarities

Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.

Post-translational modifications

Phosphorylation at Ser-365 by SGK1 inhibits its activity (Ref.8, PubMed:11410590). Phosphorylation at Thr-753 by MAPK1 (PubMed:19710016). Dephosphorylation of Ser-365 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC); this relieves inactivation and stimulates kinase activity (PubMed:35768504, PubMed:35830882).

Subcellular localisation

Nucleus

Storage

Shipped at conditions

Dry Ice

Appropriate short-term storage conditions

-80°C

Appropriate long-term storage conditions

-80°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Product promise

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In the unlikely event of one of our products not working as expected, you are covered by our product promise.

Full details and terms and conditions can be found here:
Terms & Conditions.

2 product images

  • SDS-PAGE - Recombinant human BRAF protein (ab204208), expandable thumbnail

    SDS-PAGE - Recombinant human BRAF protein (ab204208)

    The purity of ab204208 was determined to be >90% by densitometry.
    Approximate MWt: 69 kDa.

  • Functional Studies - Recombinant human BRAF protein (ab204208), expandable thumbnail

    Functional Studies - Recombinant human BRAF protein (ab204208)

    Kinase assay showing the specific activity of ab204208 as 2,300 nmol/min/mg.

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Product protocols

For this product, it's our understanding that no specific protocols are required. You can:

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