Recombinant human BRAF protein is a Human Fragment protein, in the 381 to 766 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
>90% Densitometry
Baculovirus infected Sf9 cells
GST tag N-Terminus
SDS-PAGE, FuncS
Yes
D L I R D Q G F R G D G G S T T G L S A T P P A S L P G S L T N V K A L Q K S P G P Q R E R K S S S S S E D R N R M K T L G R R D S S D D W E I P D G Q I T V G Q R I G S G S F G T V Y K G K W H G D V A V K M L N V T A P T P Q Q L Q A F K N E V G V L R K T R H V N I L L F M G Y S T K P Q L A I V T Q W C E G S S L Y H H L H I I E T K F E M I K L I D I A R Q T A Q G M D Y L H A K S I I H R D L K S N N I F L H E D L T V K I G D F G L A T V K S R W S G S H Q F E Q L S G S I L W M A P E V I R M Q D K N P Y S F Q S D V Y A F G I V L Y E L M T G Q L P Y S N I N N R D Q I I F M V G R G Y L S P D L S K V R S N C P K A M K R L M A E C L K K K R D E R P L F P Q I L A S I E L L A R S L P K I H R S A S E P S L N R A G F Q T E D F S L Y A C A S P K T P I Q A G G Y G A F P V H
Application | Reactivity | Dilution info | Notes |
---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). Phosphorylates PFKFB2 (PubMed:36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).
BRAF1, RAFB1, BRAF1, BRAF, RAFB1, Serine/threonine-protein kinase B-raf, Proto-oncogene B-Raf, p94, v-Raf murine sarcoma viral oncogene homolog B1
Recombinant human BRAF protein is a Human Fragment protein, in the 381 to 766 aa range, expressed in Baculovirus infected Sf9, with >90% purity and suitable for SDS-PAGE, FuncS.
>90% Densitometry
Baculovirus infected Sf9 cells
GST tag N-Terminus
SDS-PAGE, FuncS
Yes
The specific activity of ab204208 was determined to be 2,300 nmol/min/mg.
No
Human
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.87% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
D L I R D Q G F R G D G G S T T G L S A T P P A S L P G S L T N V K A L Q K S P G P Q R E R K S S S S S E D R N R M K T L G R R D S S D D W E I P D G Q I T V G Q R I G S G S F G T V Y K G K W H G D V A V K M L N V T A P T P Q Q L Q A F K N E V G V L R K T R H V N I L L F M G Y S T K P Q L A I V T Q W C E G S S L Y H H L H I I E T K F E M I K L I D I A R Q T A Q G M D Y L H A K S I I H R D L K S N N I F L H E D L T V K I G D F G L A T V K S R W S G S H Q F E Q L S G S I L W M A P E V I R M Q D K N P Y S F Q S D V Y A F G I V L Y E L M T G Q L P Y S N I N N R D Q I I F M V G R G Y L S P D L S K V R S N C P K A M K R L M A E C L K K K R D E R P L F P Q I L A S I E L L A R S L P K I H R S A S E P S L N R A G F Q T E D F S L Y A C A S P K T P I Q A G G Y G A F P V H
Fragment
69 kDa
381 to 766
Recombinant
GST tag N-Terminus
Liquid
Purity is lot specific. Please contact our technical Support team for details. Affinity purified.
Protein kinase involved in the transduction of mitogenic signals from the cell membrane to the nucleus (Probable). Phosphorylates MAP2K1, and thereby activates the MAP kinase signal transduction pathway (PubMed:21441910, PubMed:29433126). Phosphorylates PFKFB2 (PubMed:36402789). May play a role in the postsynaptic responses of hippocampal neurons (PubMed:1508179).
Belongs to the protein kinase superfamily. TKL Ser/Thr protein kinase family. RAF subfamily.
Phosphorylation at Ser-365 by SGK1 inhibits its activity (Ref.8, PubMed:11410590). Phosphorylation at Thr-753 by MAPK1 (PubMed:19710016). Dephosphorylation of Ser-365 by the SHOC2-MRAS-PP1c (SMP) complex consisting of SHOC2, GTP-bound M-Ras/MRAS and the catalytic subunit of protein phosphatase 1 (PPP1CA, PPP1CB or PPP1CC); this relieves inactivation and stimulates kinase activity (PubMed:35768504, PubMed:35830882).
Nucleus
Dry Ice
-80°C
-80°C
Upon delivery aliquot
Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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Terms & Conditions.
The purity of ab204208 was determined to be >90% by densitometry.
Approximate MWt: 69 kDa.
Kinase assay showing the specific activity of ab204208 as 2,300 nmol/min/mg.
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