Recombinant Human BRD2 protein is a Human Fragment protein, in the 1 to 455 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M L Q N V T P H N K L P G E G N A G L L G L G P E A A A P G K R I R K P S L L Y E G F E S P T M A S V P A L Q L T P A N P P P P E V S N P K K P G R V T N Q L Q Y L H K V V M K A L W K H Q F A W P F R Q P V D A V K L G L P D Y H K I I K Q P M D M G T I K R R L E N N Y Y W A A S E C M Q D F N T M F T N C Y I Y N K P T D D I V L M A Q T L E K I F L Q K V A S M P Q E E Q E L V V T I P K N S H K K G A K L A A L Q G S V T S A H Q V P A V S S V S H T A L Y T P P P E I P T T V L N I P H P S V I S S P L L K S L H S A G P P L L A V T A A P P A Q P L A K K K G V K R K A D T T T P T P T A I L A P G S P A S P P G S L E P K A A R L P P M R R E S G R P I K P P R K D L P D S Q Q Q H Q S S K K G K L S E Q L K H C N G I L K E L L S K K H A A Y A W P F Y K P V D A S A L G L H D Y H D I I K H P M D L S T V K R K M E N R D Y R D A Q E F A A D V R L M F S N C Y K Y N P P D H D V V A M A R K L Q D V F E F R Y A K M P D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Chromatin reader protein that specifically recognizes and binds histone H4 acetylated at 'Lys-5' and 'Lys-12' (H4K5ac and H4K12ac, respectively), thereby controlling gene expression and remodeling chromatin structures (PubMed:17148447, PubMed:17848202, PubMed:18406326, PubMed:20048151, PubMed:20709061, PubMed:20871596). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed:28262505). Plays a key role in genome compartmentalization via its association with CTCF and cohesin: recruited to chromatin by CTCF and promotes formation of topologically associating domains (TADs) via its ability to bind acetylated histones, contributing to CTCF boundary formation and enhancer insulation (PubMed:35410381). Also recognizes and binds acetylated non-histone proteins, such as STAT3 (PubMed:28262505). Involved in inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17: recognizes and binds STAT3 acetylated at 'Lys-87', promoting STAT3 recruitment to chromatin (PubMed:28262505). In addition to acetylated lysines, also recognizes and binds lysine residues on histones that are both methylated and acetylated on the same side chain to form N6-acetyl-N6-methyllysine (Kacme), an epigenetic mark of active chromatin associated with increased transcriptional initiation (PubMed:37731000). Specifically binds histone H4 acetyl-methylated at 'Lys-5' and 'Lys-12' (H4K5acme and H4K12acme, respectively) (PubMed:37731000).
Alternative names
KIAA9001, RING3, BRD2, Bromodomain-containing protein 2, O27.1.1
Recommended products
Recombinant Human BRD2 protein is a Human Fragment protein, in the 1 to 455 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P25440-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 89% PBS, 10% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M L Q N V T P H N K L P G E G N A G L L G L G P E A A A P G K R I R K P S L L Y E G F E S P T M A S V P A L Q L T P A N P P P P E V S N P K K P G R V T N Q L Q Y L H K V V M K A L W K H Q F A W P F R Q P V D A V K L G L P D Y H K I I K Q P M D M G T I K R R L E N N Y Y W A A S E C M Q D F N T M F T N C Y I Y N K P T D D I V L M A Q T L E K I F L Q K V A S M P Q E E Q E L V V T I P K N S H K K G A K L A A L Q G S V T S A H Q V P A V S S V S H T A L Y T P P P E I P T T V L N I P H P S V I S S P L L K S L H S A G P P L L A V T A A P P A Q P L A K K K G V K R K A D T T T P T P T A I L A P G S P A S P P G S L E P K A A R L P P M R R E S G R P I K P P R K D L P D S Q Q Q H Q S S K K G K L S E Q L K H C N G I L K E L L S K K H A A Y A W P F Y K P V D A S A L G L H D Y H D I I K H P M D L S T V K R K M E N R D Y R D A Q E F A A D V R L M F S N C Y K Y N P P D H D V V A M A R K L Q D V F E F R Y A K M P D
- Accession
- P25440
- Protein length
- Fragment
- Predicted molecular weight
- 52.8 kDa
- Amino acids
- 1 to 455
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Chromatin reader protein that specifically recognizes and binds histone H4 acetylated at 'Lys-5' and 'Lys-12' (H4K5ac and H4K12ac, respectively), thereby controlling gene expression and remodeling chromatin structures (PubMed:17148447, PubMed:17848202, PubMed:18406326, PubMed:20048151, PubMed:20709061, PubMed:20871596). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed:28262505). Plays a key role in genome compartmentalization via its association with CTCF and cohesin: recruited to chromatin by CTCF and promotes formation of topologically associating domains (TADs) via its ability to bind acetylated histones, contributing to CTCF boundary formation and enhancer insulation (PubMed:35410381). Also recognizes and binds acetylated non-histone proteins, such as STAT3 (PubMed:28262505). Involved in inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17: recognizes and binds STAT3 acetylated at 'Lys-87', promoting STAT3 recruitment to chromatin (PubMed:28262505). In addition to acetylated lysines, also recognizes and binds lysine residues on histones that are both methylated and acetylated on the same side chain to form N6-acetyl-N6-methyllysine (Kacme), an epigenetic mark of active chromatin associated with increased transcriptional initiation (PubMed:37731000). Specifically binds histone H4 acetyl-methylated at 'Lys-5' and 'Lys-12' (H4K5acme and H4K12acme, respectively) (PubMed:37731000).
- Sequence similarities
Belongs to the BET family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
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1 product image
SDS-PAGE - Recombinant Human BRD2 protein (ab187435)
15% SDS-PAGE analysis of ab187435 (3 μg).
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