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AB196109

Recombinant human BRD2 protein (His tag N-Terminus)

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Recombinant human BRD2 protein (His tag N-Terminus) is a Human Fragment protein, in the 65 to 187 aa range, expressed in Escherichia coli, with >98%, suitable for SDS-PAGE, FuncS.

View Alternative Names

KIAA9001, RING3, BRD2, Bromodomain-containing protein 2, O27.1.1

2 Images
Functional Studies - Recombinant human BRD2 protein (His tag N-Terminus) (AB196109)
  • FuncS

Supplier Data

Functional Studies - Recombinant human BRD2 protein (His tag N-Terminus) (AB196109)

ab196109 binding to biotinylated acetyl-histone peptide substrate.

SDS-PAGE - Recombinant human BRD2 protein (His tag N-Terminus) (AB196109)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human BRD2 protein (His tag N-Terminus) (AB196109)

4-20% SDS-PAGE analysis of 20 μg ab196109 with Coomassie staining.

Key facts

Purity

>98% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

BRD2 is incubated with biotinylated acetyl-histone peptide substrate in BRD assay buffer in a 10 μL reaction for 1 hour at RT.

Accession

P25440

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.72% Sodium chloride, 0.71% Tris HCl, 0.02% Potassium chloride

storage-buffer

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Complementary Products

Proteins & Peptides

AB176936

Recombinant Human Brd4 protein

SDS-PAGE - Recombinant Human Brd4 protein (AB176936)

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Primary Antibodies

AB50818

Anti-BRD3 antibody [2088C3a]

KO Validated

Flow Cytometry - Anti-BRD3 antibody [2088C3a] (AB50818)

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Primary Antibodies

AB139690

Anti-BRD2 antibody [EPR7642] - ChIP Grade

RabMAb|Recombinant|KO Validated

Immunocytochemistry/ Immunofluorescence - Anti-BRD2 antibody [EPR7642] - ChIP Grade (AB139690)

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Primary Antibodies

AB128874

Anti-Brd4 antibody [EPR5150(2)]

BOND RX™ Validated|RabMAb|Recombinant|Lab Essentials

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Brd4 antibody [EPR5150(2)] (AB128874)

  • 5
  • 18
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"EVSNPKKPGRVTNQLQYLHKVVMKALWKHQFAWPFRQPVDAVKLGLPDYHKIIKQPMDMGTIKRRLENNYYWAASECMQDFNTMFTNCYIYNKPTDDIVLMAQTLEKIFLQKVASMPQEEQEL","proteinLength":"Fragment","predictedMolecularWeight":"15.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":187,"aminoAcidStart":65,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P25440","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

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General info

Function

Chromatin reader protein that specifically recognizes and binds histone H4 acetylated at 'Lys-5' and 'Lys-12' (H4K5ac and H4K12ac, respectively), thereby controlling gene expression and remodeling chromatin structures (PubMed : 17148447, PubMed : 17848202, PubMed : 18406326, PubMed : 20048151, PubMed : 20709061, PubMed : 20871596). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed : 28262505). Plays a key role in genome compartmentalization via its association with CTCF and cohesin : recruited to chromatin by CTCF and promotes formation of topologically associating domains (TADs) via its ability to bind acetylated histones, contributing to CTCF boundary formation and enhancer insulation (PubMed : 35410381). Also recognizes and binds acetylated non-histone proteins, such as STAT3 (PubMed : 28262505). Involved in inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 : recognizes and binds STAT3 acetylated at 'Lys-87', promoting STAT3 recruitment to chromatin (PubMed : 28262505). In addition to acetylated lysines, also recognizes and binds lysine residues on histones that are both methylated and acetylated on the same side chain to form N6-acetyl-N6-methyllysine (Kacme), an epigenetic mark of active chromatin associated with increased transcriptional initiation (PubMed : 37731000). Specifically binds histone H4 acetyl-methylated at 'Lys-5' and 'Lys-12' (H4K5acme and H4K12acme, respectively) (PubMed : 37731000).

Sequence similarities

Belongs to the BET family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Chromatin reader protein that specifically recognizes and binds histone H4 acetylated at 'Lys-5' and 'Lys-12' (H4K5ac and H4K12ac, respectively), thereby controlling gene expression and remodeling chromatin structures (PubMed : 17148447, PubMed : 17848202, PubMed : 18406326, PubMed : 20048151, PubMed : 20709061, PubMed : 20871596). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed : 28262505). Plays a key role in genome compartmentalization via its association with CTCF and cohesin : recruited to chromatin by CTCF and promotes formation of topologically associating domains (TADs) via its ability to bind acetylated histones, contributing to CTCF boundary formation and enhancer insulation (PubMed : 35410381). Also recognizes and binds acetylated non-histone proteins, such as STAT3 (PubMed : 28262505). Involved in inflammatory response by regulating differentiation of naive CD4(+) T-cells into T-helper Th17 : recognizes and binds STAT3 acetylated at 'Lys-87', promoting STAT3 recruitment to chromatin (PubMed : 28262505). In addition to acetylated lysines, also recognizes and binds lysine residues on histones that are both methylated and acetylated on the same side chain to form N6-acetyl-N6-methyllysine (Kacme), an epigenetic mark of active chromatin associated with increased transcriptional initiation (PubMed : 37731000). Specifically binds histone H4 acetyl-methylated at 'Lys-5' and 'Lys-12' (H4K5acme and H4K12acme, respectively) (PubMed : 37731000).
See full target information BRD2
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Product promise

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