Recombinant human BRD3 protein is a Human Fragment protein, in the 29 to 417 aa range, expressed in Escherichia coli, with >=37% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >=37% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
M H H H H H H P S K P G R K T N Q L Q Y M Q N V V V K T L W K H Q F A W P F Y Q P V D A I K L N L P D Y H K I I K N P M D M G T I K K R L E N N Y Y W S A S E C M Q D F N T M F T N C Y I Y N K P T D D I V L M A Q A L E K I F L Q K V A Q M P Q E E V E L L P P A P K G K G R K P A A G A Q S A G T Q Q V A A V S S V S P A T P F Q S V P P T V S Q T P V I A A T P V P T I T A N V T S V P V P P A A A P P P P A T P I V P V V P P T P P V V K K K G V K R K A D T T T P T T S A I T A S R S E S P P P L S D P K Q A K V V A R R E S G G R P I K P P K K D L E D G E V P Q H A G K K G K L S E H L R Y C D S I L R E M L S K K H A A Y A W P F Y K P V D A E A L E L H D Y H D I I K H P M D L S T V K R K M D G R E Y P D A Q G F A A D V R L M F S N C Y K Y N P P D H E V V A M A R K L Q D V F E M R F A K M
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes Useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling. |
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Target data
Function
Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures (PubMed:18406326, PubMed:22464331, PubMed:27105114, PubMed:32895492). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed:29567837, PubMed:32895492). In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (PubMed:18406326). Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT: BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression (PubMed:32895492). Also binds non-histones acetylated proteins, such as GATA1 and GATA2: regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity).
Alternative names
KIAA0043, RING3L, BRD3, Bromodomain-containing protein 3, RING3-like protein
Recommended products
Recombinant human BRD3 protein is a Human Fragment protein, in the 29 to 417 aa range, expressed in Escherichia coli, with >=37% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >=37% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- Active
- Accession
- Q15059-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2
Constituents: 20% Glycerol (glycerin, glycerine), 1.68% Sodium chloride, 0.95% HEPES
Sequence info
Amino acid sequence
- M H H H H H H P S K P G R K T N Q L Q Y M Q N V V V K T L W K H Q F A W P F Y Q P V D A I K L N L P D Y H K I I K N P M D M G T I K K R L E N N Y Y W S A S E C M Q D F N T M F T N C Y I Y N K P T D D I V L M A Q A L E K I F L Q K V A Q M P Q E E V E L L P P A P K G K G R K P A A G A Q S A G T Q Q V A A V S S V S P A T P F Q S V P P T V S Q T P V I A A T P V P T I T A N V T S V P V P P A A A P P P P A T P I V P V V P P T P P V V K K K G V K R K A D T T T P T T S A I T A S R S E S P P P L S D P K Q A K V V A R R E S G G R P I K P P K K D L E D G E V P Q H A G K K G K L S E H L R Y C D S I L R E M L S K K H A A Y A W P F Y K P V D A E A L E L H D Y H D I I K H P M D L S T V K R K M D G R E Y P D A Q G F A A D V R L M F S N C Y K Y N P P D H E V V A M A R K L Q D V F E M R F A K M
- Accession
- Q15059
- Protein length
- Fragment
- Predicted molecular weight
- 43.95 kDa
- Amino acids
- 29 to 417
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Chromatin reader that recognizes and binds acetylated histones, thereby controlling gene expression and remodeling chromatin structures (PubMed:18406326, PubMed:22464331, PubMed:27105114, PubMed:32895492). Recruits transcription factors and coactivators to target gene sites, and activates RNA polymerase II machinery for transcriptional elongation (PubMed:29567837, PubMed:32895492). In vitro, binds acetylated lysine residues on the N-terminus of histone H2A, H2B, H3 and H4 (PubMed:18406326). Involved in endoderm differentiation via its association with long non-coding RNA (lncRNA) DIGIT: BRD3 undergoes liquid-liquid phase separation upon binding to lncRNA DIGIT, promoting binding to histone H3 acetylated at 'Lys-18' (H3K18ac) to induce endoderm gene expression (PubMed:32895492). Also binds non-histones acetylated proteins, such as GATA1 and GATA2: regulates transcription by promoting the binding of the transcription factor GATA1 to its targets (By similarity).
- Sequence similarities
Belongs to the BET family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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2 product images
SDS-PAGE - Recombinant human BRD3 protein (ab196096)
SDS PAGE analysis of ab196096.
Functional Studies - Recombinant human BRD3 protein (ab196096)
BRD3 protein is incubated with biotinylated acetyl-histone peptide substrate in BRD assay buffer in a 10 μl reaction for 1 hour at RT. Ni acceptor beads are added, followed by Streptavidin-conjugated donor beads. After 1 hour incubation at RT, Alphacounts are measured.
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