Recombinant human BRDT protein is a Human Fragment protein, in the 22 to 138 aa range, expressed in Escherichia coli, with >97% purity and suitable for SDS-PAGE, FuncS.
Images
Key facts
- Purity
- >97% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
T K K N G R L T N Q L Q Y L Q K V V L K D L W K H S F S W P F Q R P V D A V K L Q L P D Y Y T I I K N P M D L N T I K K R L E N K Y Y A K A S E C I E D F N T M F S N C Y L Y N K P G D D I V L M A Q A L E K L F M Q K L S Q M P Q E E Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis (PubMed:22464331, PubMed:22901802). Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time (PubMed:22901802). In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA (PubMed:22901802). Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac) (By similarity). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids (By similarity). Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.
Alternative names
Bromodomain testis-specific protein, Cancer/testis antigen 9, RING3-like protein, CT9, BRDT
Recommended products
Recombinant human BRDT protein is a Human Fragment protein, in the 22 to 138 aa range, expressed in Escherichia coli, with >97% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >97% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- BRDT protein is incubated with biotinylated acetyl-histone peptide substrate in BRD assay buffer in a 10 μl reaction for 1 hour at RT. GSH acceptor beads are added, followed by Streptavidin-conjugated donor beads. After 1 hour incubation at RT, Alphacounts are measured.
- Accession
- Q58F21-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.8% Sodium chloride, 0.79% Tris HCl, 0.03% EDTA, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride
Sequence info
Amino acid sequence
- T K K N G R L T N Q L Q Y L Q K V V L K D L W K H S F S W P F Q R P V D A V K L Q L P D Y Y T I I K N P M D L N T I K K R L E N K Y Y A K A S E C I E D F N T M F S N C Y L Y N K P G D D I V L M A Q A L E K L F M Q K L S Q M P Q E E Q
- Accession
- Q58F21
- Protein length
- Fragment
- Predicted molecular weight
- 40.7 kDa
- Amino acids
- 22 to 138
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis (PubMed:22464331, PubMed:22901802). Required in late pachytene spermatocytes: plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time (PubMed:22901802). In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA (PubMed:22901802). Also recognizes and binds a subset of butyrylated histones: able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac) (By similarity). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids (By similarity). Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.
- Sequence similarities
Belongs to the BET family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
ab198111 is useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
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2 product images
Functional Studies - Recombinant human BRDT protein (ab198111)
Activity assay for ab198111.
SDS-PAGE - Recombinant human BRDT protein (ab198111)
4-20% SDS-PAGE analysis of ab198111.
Lane 1: 0.5 μg ab198111
Lane 2: Protein markerStained with Coomassie Blue
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Product protocols
- Visit the general protocols
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