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AB198111

Recombinant human BRDT protein (GST tag N-Terminus)

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Recombinant human BRDT protein (GST tag N-Terminus) is a Human Fragment protein, in the 22 to 138 aa range, expressed in Escherichia coli, with >97%, suitable for SDS-PAGE, FuncS.

View Alternative Names

Bromodomain testis-specific protein, Cancer/testis antigen 9, RING3-like protein, CT9, BRDT

2 Images
Functional Studies - Recombinant human BRDT protein (GST tag N-Terminus) (AB198111)
  • FuncS

Supplier Data

Functional Studies - Recombinant human BRDT protein (GST tag N-Terminus) (AB198111)

Activity assay for ab198111.

SDS-PAGE - Recombinant human BRDT protein (GST tag N-Terminus) (AB198111)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant human BRDT protein (GST tag N-Terminus) (AB198111)

4-20% SDS-PAGE analysis of ab198111.

Lane 1 : 0.5 μg ab198111
Lane 2 : Protein marker

Stained with Coomassie Blue

Key facts

Purity

>97% SDS-PAGE

Expression system

Escherichia coli

Tags

GST tag N-Terminus

Applications

SDS-PAGE, FuncS

applications

Biologically active

Yes

Biological activity

BRDT protein is incubated with biotinylated acetyl-histone peptide substrate in BRD assay buffer in a 10 μl reaction for 1 hour at RT. GSH acceptor beads are added, followed by Streptavidin-conjugated donor beads. After 1 hour incubation at RT, Alphacounts are measured.

Accession

Q58F21

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.8% Sodium chloride, 0.79% Tris HCl, 0.03% EDTA, 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% Potassium chloride

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Complementary Products

Primary Antibodies

AB288435

Anti-BRDT antibody [EPR25427-24]

BOND RX™ Validated|20ul selling size|RabMAb|Recombinant

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-BRDT antibody [EPR25427-24] (AB288435)

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

ab198111 is useful for the study of bromodomain binding assays, screening inhibitors, and selectivity profiling.
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Sequence info

[{"sequence":"TKKNGRLTNQLQYLQKVVLKDLWKHSFSWPFQRPVDAVKLQLPDYYTIIKNPMDLNTIKKRLENKYYAKASECIEDFNTMFSNCYLYNKPGDDIVLMAQALEKLFMQKLSQMPQEEQ","proteinLength":"Fragment","predictedMolecularWeight":"40.7 kDa","actualMolecularWeight":null,"aminoAcidEnd":138,"aminoAcidStart":22,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q58F21","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Specifications

Form

Liquid

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General info

Function

Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis (PubMed : 22464331, PubMed : 22901802). Required in late pachytene spermatocytes : plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time (PubMed : 22901802). In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA (PubMed : 22901802). Also recognizes and binds a subset of butyrylated histones : able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac) (By similarity). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids (By similarity). Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.

Sequence similarities

Belongs to the BET family.

Post-translational modifications

Ubiquitinated in a SPOP-dependent manner, leading to proteasomal degradation.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Testis-specific chromatin protein that specifically binds histone H4 acetylated at 'Lys-5' and 'Lys-8' (H4K5ac and H4K8ac, respectively) and plays a key role in spermatogenesis (PubMed : 22464331, PubMed : 22901802). Required in late pachytene spermatocytes : plays a role in meiotic and post-meiotic cells by binding to acetylated histones at the promoter of specific meiotic and post-meiotic genes, facilitating their activation at the appropriate time (PubMed : 22901802). In the post-meiotic phase of spermatogenesis, binds to hyperacetylated histones and participates in their general removal from DNA (PubMed : 22901802). Also recognizes and binds a subset of butyrylated histones : able to bind histone H4 butyrylated at 'Lys-8' (H4K8ac), while it is not able to bind H4 butyrylated at 'Lys-5' (H4K5ac) (By similarity). Also acts as a component of the splicing machinery in pachytene spermatocytes and round spermatids and participates in 3'-UTR truncation of specific mRNAs in post-meiotic spermatids (By similarity). Required for chromocenter organization, a structure comprised of peri-centromeric heterochromatin.
See full target information BRDT
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