Recombinant human BRG1 protein is a Human Full Length protein, expressed in Baculovirus, with >95% purity and suitable for WB, FuncS.
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- Nature chemistry 13:626-6332021Chemical profiling of DNA G-quadruplex-interacting proteins in live cells.Applications:Unspecified applicationReactive species:Unspecified reactive speciesXiaoyun Zhang,Jochen Spiegel,Sergio Martínez Cuesta,Santosh Adhikari,Shankar BalasubramanianPubMed 34183817
- Molecular cell 67:1001-1012.e62017The Short Isoform of BRD4 Promotes HIV-1 Latency by Engaging Repressive SWI/SNF Chromatin-Remodeling Complexes.Applications:Unspecified applicationReactive species:Unspecified reactive speciesRyan J Conrad et. al.PubMed 28844864
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus
- Tags
- His tag N-Terminus
- Applications
- WB, FuncS
- Biologically active
- Yes
Amino acid sequence
M S Y Y H H H H H H D Y D I P T T E N L Y F Q G A M G I R N S K A Y V D G A G G S C R R P L S A A P V K M S T P D P P L G G T P R P G P S P G P G P S P G A M L G P S P G P S P G S A H S M M G P S P G P P S A G H P I P T Q G P G G Y P Q D N M H Q M H K P M E S M H E K G M S D D P R Y N Q M K G M G M R S G G H A G M G P P P S P M D Q H S Q G Y P S P L G G S E H A S S P V P A S G P S S G P Q M S S G P G G A P L D G A D P Q A L G Q Q N R G P T P F N Q N Q L H Q L R A Q I M A Y K M L A R G Q P L P D H L Q M A V Q G K R P M P G M Q Q Q M P T L P P P S V S A T G P G P G P G P G P G P G P G P A P P N Y S R P H G M G G P N M P P P G P S G V P P G M P G Q P P G G P P K P W P E G P M A N A A A P T S T P Q K L I P P Q P T G R P S P A P P A V P P A A S P V M P P Q T Q S P G Q P A Q P A P M V P L H Q K Q S R I T P I Q K P R G L D P V E I L Q E R E Y R L Q A R I A H R I Q E L E N L P G S L A G D L R T K A T I E L K A L R L L N F Q R Q L R Q E V V V C M R R D T A L E T A L N A K A Y K R S K R Q S L R E A R I T E K L E K Q Q K I E Q E R K R R Q K H Q E Y L N S I L Q H A K D F K E Y H R S V T G K I Q K L T K A V A T Y H A N T E R E Q K K E N E R I E K E R M R R L M A E D E E G Y R K L I D Q K K D K R L A Y L L Q Q T D E Y V A N L T E L V R Q H K A A Q V A K E K K K K K K K K K A E N A E G Q T P A I G P D G E P L D E T S Q M S D L P V K V I H V E S G K I L T G T D A P K A G Q L E A W L E M N P G Y E V A P R S D S E E S G S E E E E E E E E E E Q P Q A A Q P P T L P V E E K K K I P D P D S D D V S E V D A R H I I E N A K Q D V D D E Y G V S Q A L A R G L Q S Y Y A V A H A V T E R V D K Q S A L M V N G V L K Q Y Q I K G L E W L V S L Y N N N L N G I L A D E M G L G K T I Q T I A L I T Y L M E H K R I N G P F L I I V P L S T L S N W A Y E F D K W A P S V V K V S Y K G S P A A R R A F V P Q L R S G K F N V L L T T Y E Y I I K D K H I L A K I R W K Y M I V D E G H R M K N H H C K L T Q V L N T H Y V A P R R L L L T G T P L Q N K L P E L W A L L N F L L P T I F K S C S T F E Q W F N A P F A M T G E K V D L N E E E T I L I I R R L H K V L R P F L L R R L K K E V E A Q L P E K V E Y V I K C D M S A L Q R V L Y R H M Q A K G V L L T D G S E K D K K G K G G T K T L M N T I M Q L R K I C N H P Y M F Q H I E E S F S E H L G F T G G I V Q G L D L Y R A S G K F E L L D R I L P K L R A T N H K V L L F C Q M T S L M T I M E D Y F A Y R G F K Y L R L D G T T K A E D R G M L L K T F N E P G S E Y F I F L L S T R A G G L G L N L Q S A D T V I I F D S D W N P H Q D L Q A Q D R A H R I G Q Q N E V R V L R L C T V N S V E E K I L A A A K Y K L N V D Q K V I Q A G M F D Q K S S S H E R R A F L Q A I L E H E E Q D E S R H C S T G S G S A S F A H T A P P P A G V N P D L E E P P L K E E D E V P D D E T V N Q M I A R H E E E F D L F M R M D L D R R R E E A R N P K R K P R L M E E D E L P S W I I K D D A E V E R L T C E E E E E K M F G R G S R H R K E V D Y S D S L T E K Q W L K A I E E G T L E E I E E E V R Q K K S S R K R K R D S D A G S S T P T T S T R S R D K D D E S K K Q K K R G R P P A E K L S P N P P N L T K K M K K I V D A V I K Y K D S S S G R Q L S E V F I Q L P S R K E L P E Y Y E L I R K P V D F K K I K E R I R N H K Y R S L N D L E K D V M L L C Q N A Q T F N L E G S L I Y E D S I V L Q S V F T S V R Q K I E K E D D S E G E E S E E E E E G E E E G S E S E S R S V K V K I K L G R K E K A Q D R L K G G R R R P S R G S R A K P V V S D D D S E E E Q E E D R S G S G S E E D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application WB | Reactivity Reacts | Dilution info - | Notes ab83327 can be used as a WB positive control in conjunction with Anti-BRG1 antibody ab4081. |
Application FuncS | Reactivity Reacts | Dilution info - | Notes in vitro transcription assay: Use at an assay dependent dilution.in vitro nucleosome remodeling assay: Use 50-100 units.Cell growth assay: Use at an assay dependent dilution.1 unit equals 1 nanogram purified protein. |
Associated Products
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Target data
Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development, a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Binds via DLX1 to enhancers located in the intergenic region between DLX5 and DLX6 and this binding is stabilized by the long non-coding RNA (lncRNA) Evf2 (By similarity). Binds to RNA in a promiscuous manner (By similarity). Binding to RNAs including lncRNA Evf2 leads to inhibition of SMARCA4 ATPase and chromatin remodeling activities (By similarity). In brown adipose tissue, involved in the regulation of thermogenic genes expression (By similarity).
Alternative names
BAF190A, BRG1, SNF2B, SNF2L4, SMARCA4, Transcription activator BRG1, ATP-dependent helicase SMARCA4, BRG1-associated factor 190A, Mitotic growth and transcription activator, Protein BRG-1, Protein brahma homolog 1, SNF2-beta, SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4
Recommended products
Recombinant human BRG1 protein is a Human Full Length protein, expressed in Baculovirus, with >95% purity and suitable for WB, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Baculovirus
- Tags
- His tag N-Terminus
- Applications
- WB, FuncS
- Biologically active
- Yes
- Biological activity
- ab82237 contains no detectable proteases, DNase and RNase activity.
- Accession
- P51532-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.9
Constituents: 20% Glycerol (glycerin, glycerine), 0.75% Potassium chloride, 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.00584% EDTA
Sequence info
Amino acid sequence
- M S Y Y H H H H H H D Y D I P T T E N L Y F Q G A M G I R N S K A Y V D G A G G S C R R P L S A A P V K M S T P D P P L G G T P R P G P S P G P G P S P G A M L G P S P G P S P G S A H S M M G P S P G P P S A G H P I P T Q G P G G Y P Q D N M H Q M H K P M E S M H E K G M S D D P R Y N Q M K G M G M R S G G H A G M G P P P S P M D Q H S Q G Y P S P L G G S E H A S S P V P A S G P S S G P Q M S S G P G G A P L D G A D P Q A L G Q Q N R G P T P F N Q N Q L H Q L R A Q I M A Y K M L A R G Q P L P D H L Q M A V Q G K R P M P G M Q Q Q M P T L P P P S V S A T G P G P G P G P G P G P G P G P A P P N Y S R P H G M G G P N M P P P G P S G V P P G M P G Q P P G G P P K P W P E G P M A N A A A P T S T P Q K L I P P Q P T G R P S P A P P A V P P A A S P V M P P Q T Q S P G Q P A Q P A P M V P L H Q K Q S R I T P I Q K P R G L D P V E I L Q E R E Y R L Q A R I A H R I Q E L E N L P G S L A G D L R T K A T I E L K A L R L L N F Q R Q L R Q E V V V C M R R D T A L E T A L N A K A Y K R S K R Q S L R E A R I T E K L E K Q Q K I E Q E R K R R Q K H Q E Y L N S I L Q H A K D F K E Y H R S V T G K I Q K L T K A V A T Y H A N T E R E Q K K E N E R I E K E R M R R L M A E D E E G Y R K L I D Q K K D K R L A Y L L Q Q T D E Y V A N L T E L V R Q H K A A Q V A K E K K K K K K K K K A E N A E G Q T P A I G P D G E P L D E T S Q M S D L P V K V I H V E S G K I L T G T D A P K A G Q L E A W L E M N P G Y E V A P R S D S E E S G S E E E E E E E E E E Q P Q A A Q P P T L P V E E K K K I P D P D S D D V S E V D A R H I I E N A K Q D V D D E Y G V S Q A L A R G L Q S Y Y A V A H A V T E R V D K Q S A L M V N G V L K Q Y Q I K G L E W L V S L Y N N N L N G I L A D E M G L G K T I Q T I A L I T Y L M E H K R I N G P F L I I V P L S T L S N W A Y E F D K W A P S V V K V S Y K G S P A A R R A F V P Q L R S G K F N V L L T T Y E Y I I K D K H I L A K I R W K Y M I V D E G H R M K N H H C K L T Q V L N T H Y V A P R R L L L T G T P L Q N K L P E L W A L L N F L L P T I F K S C S T F E Q W F N A P F A M T G E K V D L N E E E T I L I I R R L H K V L R P F L L R R L K K E V E A Q L P E K V E Y V I K C D M S A L Q R V L Y R H M Q A K G V L L T D G S E K D K K G K G G T K T L M N T I M Q L R K I C N H P Y M F Q H I E E S F S E H L G F T G G I V Q G L D L Y R A S G K F E L L D R I L P K L R A T N H K V L L F C Q M T S L M T I M E D Y F A Y R G F K Y L R L D G T T K A E D R G M L L K T F N E P G S E Y F I F L L S T R A G G L G L N L Q S A D T V I I F D S D W N P H Q D L Q A Q D R A H R I G Q Q N E V R V L R L C T V N S V E E K I L A A A K Y K L N V D Q K V I Q A G M F D Q K S S S H E R R A F L Q A I L E H E E Q D E S R H C S T G S G S A S F A H T A P P P A G V N P D L E E P P L K E E D E V P D D E T V N Q M I A R H E E E F D L F M R M D L D R R R E E A R N P K R K P R L M E E D E L P S W I I K D D A E V E R L T C E E E E E K M F G R G S R H R K E V D Y S D S L T E K Q W L K A I E E G T L E E I E E E V R Q K K S S R K R K R D S D A G S S T P T T S T R S R D K D D E S K K Q K K R G R P P A E K L S P N P P N L T K K M K K I V D A V I K Y K D S S S G R Q L S E V F I Q L P S R K E L P E Y Y E L I R K P V D F K K I K E R I R N H K Y R S L N D L E K D V M L L C Q N A Q T F N L E G S L I Y E D S I V L Q S V F T S V R Q K I E K E D D S E G E E S E E E E E G E E E G S E S E S R S V K V K I K L G R K E K A Q D R L K G G R R R P S R G S R A K P V V S D D D S E E E Q E E D R S G S G S E E D
- Accession
- P51532
- Protein length
- Full Length
- Predicted molecular weight
- 190.5 kDa
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab82237 is greater than 95% homogeneous, purified by affinity and FPLC chromatography.
General info
- Function
Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. Component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating the calcium-dependent release of a repressor complex and the recruitment of an activator complex. In resting neurons, transcription of the c-FOS promoter is inhibited by SMARCA4-dependent recruitment of a phospho-RB1-HDAC repressor complex. Upon calcium influx, RB1 is dephosphorylated by calcineurin, which leads to release of the repressor complex. At the same time, there is increased recruitment of CREBBP to the promoter by a CREST-dependent mechanism, which leads to transcriptional activation. The CREST-BRG1 complex also binds to the NR2B promoter, and activity-dependent induction of NR2B expression involves the release of HDAC1 and recruitment of CREBBP. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development, a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth. SMARCA4/BAF190A may promote neural stem cell self-renewal/proliferation by enhancing Notch-dependent proliferative signals, while concurrently making the neural stem cell insensitive to SHH-dependent differentiating cues (By similarity). Acts as a corepressor of ZEB1 to regulate E-cadherin transcription and is required for induction of epithelial-mesenchymal transition (EMT) by ZEB1. Binds via DLX1 to enhancers located in the intergenic region between DLX5 and DLX6 and this binding is stabilized by the long non-coding RNA (lncRNA) Evf2 (By similarity). Binds to RNA in a promiscuous manner (By similarity). Binding to RNAs including lncRNA Evf2 leads to inhibition of SMARCA4 ATPase and chromatin remodeling activities (By similarity). In brown adipose tissue, involved in the regulation of thermogenic genes expression (By similarity).
- Sequence similarities
Belongs to the SNF2/RAD54 helicase family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
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Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
Western blot - Recombinant human BRG1 protein (ab82237)
All lanes: Western blot - Anti-BRG1 antibody (Anti-BRG1 antibody ab4081) at 1/1000 dilution
All lanes: Western blot - Recombinant human BRG1 protein (ab82237) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 185 kDa
Exposure time: 8min
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