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AB271391

Recombinant human Brk/PTK6 protein (Tagged)

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Recombinant human Brk/PTK6 protein (Tagged) is a Human Full Length protein, in the 2 to 451 aa range, expressed in Baculovirus infected Sf9 cells, with >65%, suitable for SDS-PAGE, FuncS.

View Alternative Names

BRK, PTK6, Protein-tyrosine kinase 6, Breast tumor kinase, Tyrosine-protein kinase BRK

2 Images
SDS-PAGE - Recombinant human Brk/PTK6 protein (Tagged) (AB271391)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant human Brk/PTK6 protein (Tagged) (AB271391)

SDS-PAGE analysis of ab271391.

Functional Studies - Recombinant human Brk/PTK6 protein (Tagged) (AB271391)
  • FuncS

Unknown

Functional Studies - Recombinant human Brk/PTK6 protein (Tagged) (AB271391)

Specific activity of ab271391.

Key facts

Purity

>65% SDS-PAGE

Expression system

Baculovirus infected Sf9 cells

Tags

GST tag N-Terminus

Applications

FuncS, SDS-PAGE

applications

Biologically active

Yes

Biological activity

Specific Activity: 88 pmol/min/μg

Assay conditions: Enzyme reaction was conducted in a buffer (25 μl) containing 40 mM Tris-HCl (pH 7.5), 20 mM MgCl2, 100 μM ATP, 0.2 mg/ml poly-(Glu, Tyr), and BRK for 40 min at 30˚C. ATP reduction is detected using ADP-Glo™ Kinase Assay Platform.

Accession

Q13882

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 20% Glycerol (glycerin, glycerine), 6% Glutathione, 0.64% Sodium chloride, 0.48% Tris, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.04% Sorbitan monolaurate, ethoxylated, 0.02% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"VSRDQAHLGPKYVGLWDFKSRTDEELSFRAGDVFHVARKEEQWWWATLLDEAGGAVAQGYVPHNYLAERETVESEPWFFGCISRSEAVRRLQAEGNATGAFLIRVSEKPSADYVLSVRDTQAVRHYKIWRRAGGRLHLNEAVSFLSLPELVNYHRAQSLSHGLRLAAPCRKHEPEPLPHWDDWERPREEFTLCRKLGSGYFGEVFEGLWKDRVQVAIKVISRDNLLHQQMLQSEIQAMKKLRHKHILALYAVVSVGDPVYIITELMAKGSLLELLRDSDEKVLPVSELLDIAWQVAEGMCYLESQNYIHRDLAARNILVGENTLCKVGDFGLARLIKEDVYLSHDHNIPYKWTAPEALSRGHYSTKSDVWSFGILLHEMFSRGQVPYPGMSNHEAFLRVDAGYRMPCPLECPPSVHKLMLTCWCRDPEQRPCFKALRERLSSFTSYENPT","proteinLength":"Full Length","predictedMolecularWeight":"80 kDa","actualMolecularWeight":null,"aminoAcidEnd":451,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Baculovirus infected Sf9 cells","accessionNumber":"Q13882","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Storage information
Avoid freeze / thaw cycle
True
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Brk known also as PTK6 is a non-receptor tyrosine kinase. Its molecular mass is approximately 52 kDa. PTK6 is expressed in various tissues with higher levels found in epithelial tissues such as breast skin and gastrointestinal tissues. Mechanically PTK6 phosphorylates different substrates affecting their function and participating in signal transduction. It acts intracellularly influencing cellular processes like proliferation differentiation and migration.
Biological function summary

PTK6 plays a role in cellular signaling systems influencing growth and survival. It does not belong to a large protein complex but often interacts with several downstream proteins to exert its effects. PTK6 modulates pathways in normal epithelial tissue development and homeostasis. In addition it regulates functions like apoptosis and motility significant for tissue maintenance and repair.

Pathways

PTK6 relates to key signaling cascades such as the MAPK and PI3K-Akt pathways. Within these pathways PTK6 is often associated with proteins like ERK1/2 and Akt which are important for cell survival and growth regulation. PTK6 can activate or inhibit these pathways influencing the cellular response to various stimuli and sometimes altering processes like cell cycle progression.

PTK6 has been implicated in the development and progression of certain cancers such as breast and prostate cancer. Elevated PTK6 expression levels have been linked to poor prognosis in these cancers where it appears to enhance tumor cell proliferation and invasion. The kinase interacts with proteins like HER2 and EGFR which are also involved in these cancers potentially affecting therapeutic responses and resistance to treatments.
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Specifications

Form

Liquid

Additional notes

Affinity purified.

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General info

Function

Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins : KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors : STAT3 and STAT5A/B and a variety of signaling molecules : ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways.. Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. BRK/PTK6/SIK subfamily.

Post-translational modifications

Autophosphorylated. Autophosphorylation of Tyr-342 leads to an increase of kinase activity. Tyr-447 binds to the SH2 domain when phosphorylated and negatively regulates kinase activity.

Subcellular localisation

Nucleus

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Product protocols

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Target data

Non-receptor tyrosine-protein kinase implicated in the regulation of a variety of signaling pathways that control the differentiation and maintenance of normal epithelia, as well as tumor growth. Function seems to be context dependent and differ depending on cell type, as well as its intracellular localization. A number of potential nuclear and cytoplasmic substrates have been identified. These include the RNA-binding proteins : KHDRBS1/SAM68, KHDRBS2/SLM1, KHDRBS3/SLM2 and SFPQ/PSF; transcription factors : STAT3 and STAT5A/B and a variety of signaling molecules : ARHGAP35/p190RhoGAP, PXN/paxillin, BTK/ATK, STAP2/BKS. Associates also with a variety of proteins that are likely upstream of PTK6 in various signaling pathways, or for which PTK6 may play an adapter-like role. These proteins include ADAM15, EGFR, ERBB2, ERBB3 and IRS4. In normal or non-tumorigenic tissues, PTK6 promotes cellular differentiation and apoptosis. In tumors PTK6 contributes to cancer progression by sensitizing cells to mitogenic signals and enhancing proliferation, anchorage-independent survival and migration/invasion. Association with EGFR, ERBB2, ERBB3 may contribute to mammary tumor development and growth through enhancement of EGF-induced signaling via BTK/AKT and PI3 kinase. Contributes to migration and proliferation by contributing to EGF-mediated phosphorylation of ARHGAP35/p190RhoGAP, which promotes association with RASA1/p120RasGAP, inactivating RhoA while activating RAS. EGF stimulation resulted in phosphorylation of PNX/Paxillin by PTK6 and activation of RAC1 via CRK/CrKII, thereby promoting migration and invasion. PTK6 activates STAT3 and STAT5B to promote proliferation. Nuclear PTK6 may be important for regulating growth in normal epithelia, while cytoplasmic PTK6 might activate oncogenic signaling pathways.. Isoform 2 inhibits PTK6 phosphorylation and PTK6 association with other tyrosine-phosphorylated proteins.
See full target information PTK6
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