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AB139205

Recombinant Human BTF3 protein

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Recombinant Human BTF3 protein is a Human Full Length protein, in the 1 to 206 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

NACB, OK/SW-cl.8, BTF3, Transcription factor BTF3, Nascent polypeptide-associated complex subunit beta, RNA polymerase B transcription factor 3, NAC-beta

1 Images
SDS-PAGE - Recombinant Human BTF3 protein (AB139205)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human BTF3 protein (AB139205)

15% SDS-PAGE analysis of ab139205 (3μg)

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

Mass Spec, SDS-PAGE

applications

Biologically active

No

Accession

P20290

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMRRTGAPAQADSRGRGRARGGCPGGEATLSQPPPRGGTRGQEPQMKETIMNQEKLAKLQAQVRIGGKGTARRKKKVVHRTATADDKKLQFSLKKLGVNNISGIEEVNMFTNQGTVIHFNNPKVQASLAANTFTITGHAETKQLTEMLPSILNQLGADSLTSLRRLAEALPKQSVDGKAPLATGEDDDDEVPDLVENFDEASKNEAN","proteinLength":"Full Length","predictedMolecularWeight":"24.6 kDa","actualMolecularWeight":null,"aminoAcidEnd":206,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P20290","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

BTF3 also known as Basic Transcription Factor 3 is an important transcription factor weighing around 21 kDa. It plays a mechanical role in initiating and regulating transcription by binding to RNA polymerase II facilitating gene expression. BTF3 is ubiquitously expressed in various tissues serving as an essential component in cellular processes. Its activity is not confined to specific cell types making it an important element across the organism.
Biological function summary

BTF3 contributes significantly to the regulation of transcription and is part of the transcriptional preinitiation complex. This complex is necessary for the transcription of class II genes influencing cell cycle and growth. Through its interaction within this complex BTF3 assists in recruiting RNA polymerase II to specific gene promoters. It plays a role in maintaining gene expression stability affecting how cells respond to different stimuli.

Pathways

BTF3 is integrally involved in the transcriptional regulation pathways. It directly participates in the transcription initiation process working with proteins such as TFIIB and TFIID within the transcriptional machinery. This interaction places BTF3 as a critical link in the gene expression regulatory network impacting the overall cellular response to environmental and physiological signals.

BTF3 has associations with several conditions including cancer and neurodegenerative diseases. In cancer the overexpression of BTF3 leads to unchecked cell proliferation linking it with tumorigenesis. It interacts with proteins such as MYC which is vital in cell cycle regulation and apoptosis. In neurodegenerative conditions the disruption of BTF3's normal function may contribute to pathological progress showing its broad relevance in both health and disease.
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Specifications

Form

Liquid

Additional notes

ab139205 was purified by using conventional chromatography techniques.

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General info

Function

When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.

Sequence similarities

Belongs to the NAC-beta family.

Subcellular localisation

Nucleus

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Product protocols

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Target data

When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
See full target information BTF3
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Primary Antibodies

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