Recombinant Human BTF3 protein is a Human Full Length protein, in the 1 to 206 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M R R T G A P A Q A D S R G R G R A R G G C P G G E A T L S Q P P P R G G T R G Q E P Q M K E T I M N Q E K L A K L Q A Q V R I G G K G T A R R K K K V V H R T A T A D D K K L Q F S L K K L G V N N I S G I E E V N M F T N Q G T V I H F N N P K V Q A S L A A N T F T I T G H A E T K Q L T E M L P S I L N Q L G A D S L T S L R R L A E A L P K Q S V D G K A P L A T G E D D D D E V P D L V E N F D E A S K N E A N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
Alternative names
NACB, OK/SW-cl.8, BTF3, Transcription factor BTF3, Nascent polypeptide-associated complex subunit beta, RNA polymerase B transcription factor 3, NAC-beta
Recommended products
Recombinant Human BTF3 protein is a Human Full Length protein, in the 1 to 206 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- P20290-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 50% Glycerol (glycerin, glycerine), 1.17% Sodium chloride, 0.32% Tris HCl, 0.03% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M R R T G A P A Q A D S R G R G R A R G G C P G G E A T L S Q P P P R G G T R G Q E P Q M K E T I M N Q E K L A K L Q A Q V R I G G K G T A R R K K K V V H R T A T A D D K K L Q F S L K K L G V N N I S G I E E V N M F T N Q G T V I H F N N P K V Q A S L A A N T F T I T G H A E T K Q L T E M L P S I L N Q L G A D S L T S L R R L A E A L P K Q S V D G K A P L A T G E D D D D E V P D L V E N F D E A S K N E A N
- Accession
- P20290
- Protein length
- Full Length
- Predicted molecular weight
- 24.6 kDa
- Amino acids
- 1 to 206
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab139205 was purified by using conventional chromatography techniques.
General info
- Function
When associated with NACA, prevents inappropriate targeting of non-secretory polypeptides to the endoplasmic reticulum (ER). Binds to nascent polypeptide chains as they emerge from the ribosome and blocks their interaction with the signal recognition particle (SRP), which normally targets nascent secretory peptides to the ER. BTF3 is also a general transcription factor that can form a stable complex with RNA polymerase II. Required for the initiation of transcription.
- Sequence similarities
Belongs to the NAC-beta family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
BTF3 also known as Basic Transcription Factor 3 is an important transcription factor weighing around 21 kDa. It plays a mechanical role in initiating and regulating transcription by binding to RNA polymerase II facilitating gene expression. BTF3 is ubiquitously expressed in various tissues serving as an essential component in cellular processes. Its activity is not confined to specific cell types making it an important element across the organism.
- Biological function summary
BTF3 contributes significantly to the regulation of transcription and is part of the transcriptional preinitiation complex. This complex is necessary for the transcription of class II genes influencing cell cycle and growth. Through its interaction within this complex BTF3 assists in recruiting RNA polymerase II to specific gene promoters. It plays a role in maintaining gene expression stability affecting how cells respond to different stimuli.
- Pathways
BTF3 is integrally involved in the transcriptional regulation pathways. It directly participates in the transcription initiation process working with proteins such as TFIIB and TFIID within the transcriptional machinery. This interaction places BTF3 as a critical link in the gene expression regulatory network impacting the overall cellular response to environmental and physiological signals.
- Associated diseases and disorders
BTF3 has associations with several conditions including cancer and neurodegenerative diseases. In cancer the overexpression of BTF3 leads to unchecked cell proliferation linking it with tumorigenesis. It interacts with proteins such as MYC which is vital in cell cycle regulation and apoptosis. In neurodegenerative conditions the disruption of BTF3's normal function may contribute to pathological progress showing its broad relevance in both health and disease.
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1 product image
SDS-PAGE - Recombinant Human BTF3 protein (ab139205)
15% SDS-PAGE analysis of ab139205 (3μg)
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