JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB139802

Recombinant Human Bub3 protein (denatured)

Be the first to review this product! Submit a review

|

(1 Publication)

Recombinant Human Bub3 protein (denatured) is a Human Full Length protein, in the 1 to 328 aa range, expressed in Escherichia coli, with >85%, suitable for SDS-PAGE.

View Alternative Names

Mitotic checkpoint protein BUB3, BUB3

1 Images
SDS-PAGE - Recombinant Human Bub3 protein (denatured) (AB139802)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Bub3 protein (denatured) (AB139802)

15% SDS-PAGE analysis of ab139802 (3μg).

Key facts

Purity

>85% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE

applications

Biologically active

No

Accession

O43684

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 2.4% Urea, 0.32% Tris HCl

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column
style
left-column

Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGSMTGSNEFKLNQPPEDGISSVKFSPNTSQFLLVSSWDTSVRLYDVPANSMRLKYQHTGAVLDCAFYDPTHAWSGGLDHQLKMHDLNTDQENLVGTHDAPIRCVEYCPEVNVMVTGSWDQTVKLWDPRTPCNAGTFSQPEKVYTLSVSGDRLIVGTAGRRVLVWDLRNMGYVQQRRESSLKYQTRCIRAFPNKQGYVLSSIEGRVAVEYLDPSPEVQKKKYAFKCHRLKENNIEQIYPVNAISFHNIHNTFATGGSDGFVNIWDPFNKKRLCQFHRYPTSIASLAFSNDGTTLAIASSYMYEMDDTEHPEDGIFIRQVTDAETKPKSPCT","proteinLength":"Full Length","predictedMolecularWeight":"39.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":328,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"O43684","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage duration
1-2 weeks
Appropriate short-term storage conditions
+4°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Bub3 also known as budding uninhibited by benzimidazoles-3 is an important component of the spindle checkpoint machinery. Weighing approximately 35 kDa this protein is expressed ubiquitously in eukaryotic cells. Bub3 interacts with other spindle checkpoint proteins and takes part in chromosome segregation during mitosis. It primarily functions in the nucleus during the cell cycle ensuring proper attachment of chromosomes to the mitotic spindle which is important for accurate cell division.
Biological function summary

Bub3 participates in the mitotic checkpoint complex an essential mediator of cell cycle arrest in response to improper spindle attachment. This complex includes Bub1 BubR1 and Mad3 where Bub3 acts as an adapter facilitating interactions within the complex. It helps maintain chromosomal stability by preventing premature anaphase onset therefore ensuring that damaged or unaligned chromosomes do not progress through cell division. By doing so Bub3 safeguards against aneuploidy and genomic instability.

Pathways

Bub3 is linked to the spindle assembly checkpoint (SAC) and the cell cycle regulation pathway. In SAC Bub3 collaborates closely with proteins such as Bub1 and Mad1 to inhibit the anaphase-promoting complex/cyclosome (APC/C) until all chromosomes are properly aligned. The interplay between Bub3 and BubR1 is important for delaying the chief exit phases of mitosis ensuring that all cellular conditions are favorable for division. These interactions prevent errors that might cause irreparable damage or cell death.

Bub3 has associations with cancer and some neurodegenerative diseases. Its dysregulation can lead to aneuploidy a hallmark of many cancers through faulty chromosome segregation. In several cancer types altered expression of Bub3 and its partners such as Bub1 and Mad2 has been documented. Similarly emerging studies suggest links between Bub3 malfunction and neurodegeneration where alterations in cell division and genomic stability may contribute to disease progression.
style
left-column

Specifications

Form

Liquid

style
left-column

General info

Function

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.

Sequence similarities

Belongs to the WD repeat BUB3 family.

Post-translational modifications

Poly-ADP-ribosylated by PARP1.. Ubiquitinated by UBR5, promoting disassembly of the mitotic checkpoint complex from the APC/C complex.

Subcellular localisation

Nucleus

style
left-column

Product protocols

For this product, it's our understanding that no specific protocols are required. You can visit:
style
left-column

Target data

Has a dual function in spindle-assembly checkpoint signaling and in promoting the establishment of correct kinetochore-microtubule (K-MT) attachments. Promotes the formation of stable end-on bipolar attachments. Necessary for kinetochore localization of BUB1. Regulates chromosome segregation during oocyte meiosis. The BUB1/BUB3 complex plays a role in the inhibition of anaphase-promoting complex or cyclosome (APC/C) when spindle-assembly checkpoint is activated and inhibits the ubiquitin ligase activity of APC/C by phosphorylating its activator CDC20. This complex can also phosphorylate MAD1L1.
See full target information Mitotic checkpoint protein BUB3
style
left-column

Publications (1)

Recent publications for all applications. Explore the full list and refine your search

Nature communications 7:12969 PubMed27694884

2016

SUMOylated NKAP is essential for chromosome alignment by anchoring CENP-E to kinetochores.

Applications

Unspecified application

Species

Unspecified reactive species

Teng Li,Liang Chen,Juanxian Cheng,Jiang Dai,Yijiao Huang,Jian Zhang,Zhaoshan Liu,Ang Li,Na Li,Hongxia Wang,Xiaomin Yin,Kun He,Ming Yu,Tao Zhou,Xuemin Zhang,Qing Xia
View all publications
style
left-column
style
left-column

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions
style
left-column
style
right-column
style
bg-slate-100

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com