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AB322693

Recombinant Human Butyrylcholinesterase Protein Standard (His tag)

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Recombinant Human Butyrylcholinesterase Protein Standard (His tag) is a Human Full Length protein, expressed in HEK 293 cells, with >80%, suitable for SDS-PAGE, sELISA.

View Alternative Names

CHE1, BCHE, Cholinesterase, Acylcholine acylhydrolase, Butyrylcholine esterase, Choline esterase II, Pseudocholinesterase

2 Images
Sandwich ELISA - Recombinant Human Butyrylcholinesterase Protein Standard (His tag) (AB322693)
  • sELISA

Supplier Data

Sandwich ELISA - Recombinant Human Butyrylcholinesterase Protein Standard (His tag) (AB322693)

Sandwich ELISA with the capture antibody dilution at 2 µg/mL and detector antibody dilution at 0.5 µg/mL.

SDS-PAGE - Recombinant Human Butyrylcholinesterase Protein Standard (His tag) (AB322693)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human Butyrylcholinesterase Protein Standard (His tag) (AB322693)

SDS-PAGE analysis of ab322693 under reducing conditions for 2ug protein.

Key facts

Purity

>80% SDS-PAGE

Expression system

HEK 293 cells

Tags

His tag N-Terminus

Applications

SDS-PAGE, sELISA

applications

Biologically active

No

Accession

P06276

Animal free

Yes

Carrier free

No

Species

Human

Storage buffer

pH: 7.2 - 7.4 Constituents: PBS

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "sELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

While the standard is the same as the one provided in the corresponding SimpleStep ELISA Kit, it cannot be treated as the consumable provided with our SimpleStep ELISA Kit due to differences in its concentration calibration.

Abcam guarantee that this protein standard is suitable for use in a sandwich ELISA. Individual results may vary due to differences in technique, laboratory equipment, buffers, and other experimental factors. The detection range provided for this protein standard is based on initial sandwich ELISA validation data.

The protein concentration is the concentration after validation on our sandwich ELISA platform. This Standard protein is guaranteed to work with our Capture and Detector antibodies in sELISA. Please contact our Scientific Supportteam to know which antibody pair is suitable for this protein.

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Sequence info

[{"sequence":null,"proteinLength":"Full Length","predictedMolecularWeight":"88.1 kDa","actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"HEK 293 cells","accessionNumber":"P06276","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Specifications

Form

Liquid

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General info

Function

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.

Sequence similarities

Belongs to the type-B carboxylesterase/lipase family.

Post-translational modifications

N-glycosylated. No other PTM detected (PubMed:20946535). The major N-glycan structures are of the complex diantennary type with 1 and 2 N-acetylneuraminic acid molecules (Neu5Ac) making up approximately 33% and 47% of the total N-glycans, respectively. Only low amounts of fucosylated diantennary N-glycans are detected (approximately 2%). Triantennary N-glycans with or without fucose amount to approximately 13%, whereas 5% of the total N-glycans are of the oligomannosidic or hybrid type.

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Product protocols

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Target data

Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
See full target information BCHE
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Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

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