Recombinant Human BVR protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M A E P E R K F G V V V V G V G R A G S V R M R D L R N P H P S S A F L N L I G F V S R R E L G S I D G V Q Q I S L E D A L S S Q E V E V A Y I C S E S S S H E D Y I R Q F L N A G K H V L V E Y P M T L S L A A A Q E L W E L A E Q K G K V L H E E H V E L L M E E F A F L K K E V V G K D L L K G S L L F T A G P L E E E R F G F P A F S G I S R L T W L V S L F G E L S L V S A T L E E R K E D Q Y M K M T V C L E T E K K S P L S W I E E K G P G L K R N R Y L S F H F K S G S L E N V P N V G V N K N I F L K D Q N I F V Q K L L G Q F S E K E L A A E K K R I L H C L G L A E E I Q K Y C C S R K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Associated Products
Select an associated product type
1 product for Alternative Product
Target data
Function
Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IXalpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor (PubMed:10858451, PubMed:7929092, PubMed:8424666, PubMed:8631357). Does not reduce bilirubin IXbeta (PubMed:10858451). Uses the reactants NADH or NADPH depending on the pH; NADH is used at the acidic pH range (6-6.9) and NADPH at the alkaline range (8.5-8.7) (PubMed:7929092, PubMed:8424666, PubMed:8631357). NADPH, however, is the probable reactant in biological systems (PubMed:7929092).
Alternative names
BLVR, BVR, BLVRA, Biliverdin reductase A, BVR A, Biliverdin-IX alpha-reductase
Recommended products
Recombinant Human BVR protein is a Human Full Length protein, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P53004-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl
Sequence info
Amino acid sequence
- M A E P E R K F G V V V V G V G R A G S V R M R D L R N P H P S S A F L N L I G F V S R R E L G S I D G V Q Q I S L E D A L S S Q E V E V A Y I C S E S S S H E D Y I R Q F L N A G K H V L V E Y P M T L S L A A A Q E L W E L A E Q K G K V L H E E H V E L L M E E F A F L K K E V V G K D L L K G S L L F T A G P L E E E R F G F P A F S G I S R L T W L V S L F G E L S L V S A T L E E R K E D Q Y M K M T V C L E T E K K S P L S W I E E K G P G L K R N R Y L S F H F K S G S L E N V P N V G V N K N I F L K D Q N I F V Q K L L G Q F S E K E L A A E K K R I L H C L G L A E E I Q K Y C C S R K
- Accession
- P53004
- Protein length
- Full Length
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
ab87761 is purified using conventional chromatography techniques.
General info
- Function
Reduces the gamma-methene bridge of the open tetrapyrrole, biliverdin IXalpha, to bilirubin with the concomitant oxidation of a NADH or NADPH cofactor (PubMed:10858451, PubMed:7929092, PubMed:8424666, PubMed:8631357). Does not reduce bilirubin IXbeta (PubMed:10858451). Uses the reactants NADH or NADPH depending on the pH; NADH is used at the acidic pH range (6-6.9) and NADPH at the alkaline range (8.5-8.7) (PubMed:7929092, PubMed:8424666, PubMed:8631357). NADPH, however, is the probable reactant in biological systems (PubMed:7929092).
- Sequence similarities
Belongs to the Gfo/Idh/MocA family. Biliverdin reductase subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
Endotoxin Level: < 1.0 EU per 1 µg of protein (determined by LAL method).Protein previously labeled as Biliverdin Reductase.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The BVR protein known in full form as Biliverdin Reductase plays an essential mechanical role in the conversion of biliverdin to bilirubin. It exists as two main isoforms BVR-A and BVR-B with molecular masses approximately of 33 kDa. BVR is widely expressed in various tissues with higher levels found in liver kidney and spleen. As an enzyme its main function centers around the redox cycle between biliverdin and bilirubin providing an antioxidant defense mechanism.
- Biological function summary
BVR influences cell signaling and growth processes acting as a multifunctional enzyme beyond its primary reductive activity. It participates in the MAPK and PI3K/Akt signaling pathways acting both as a kinase and phosphatase which affects transcription factor regulation. BVR is also involved in heme metabolism working in conjunction with heme oxygenase to recycle heme a vital cellular molecule.
- Pathways
BVR significantly contributes to the antioxidative and anti-inflammatory pathways. It operates within the heme catabolic pathway and the MAPK signaling cascade linking to proteins like heme oxygenase-1 (HO-1) and the transcription factor Nrf2. These pathways support cell response to oxidative stress and regulate gene expression in response to environmental stressors.
- Associated diseases and disorders
BVR is associated with conditions such as jaundice and chronic inflammation-oriented diseases. Its interaction with proteins like heme oxygenase-1 (HO-1) elevates its importance in managing oxidative stress-related disorders. Disruption in BVR function or expression can influence bilirubin metabolism potentially leading to hyperbilirubinemia and contributing to inflammatory disease development through altered cell signaling.
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human BVR protein (ab87761)
15% SDS-PAGE analysis of 3μg ab87761.
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com