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AB159473

Recombinant Human C-4 Methylsterol Oxidase/SC4MOL protein (GST tag N-Terminus)

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Recombinant Human C-4 Methylsterol Oxidase/SC4MOL protein (GST tag N-Terminus) is a Human Full Length protein, in the 1 to 293 aa range, expressed in Wheat germ, suitable for ELISA, WB.

View Alternative Names

DESP4, ERG25, SC4MOL, MSMO1, Methylsterol monooxygenase 1, C-4 methylsterol oxidase, Sterol-C4-methyl oxidase

1 Images
SDS-PAGE - Recombinant Human C-4 Methylsterol Oxidase/SC4MOL protein (GST tag N-Terminus) (AB159473)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human C-4 Methylsterol Oxidase/SC4MOL protein (GST tag N-Terminus) (AB159473)

ab159473 on a 12.5% SDS-PAGE stained with Coomassie Blue.

Key facts

Expression system

Wheat germ

Tags

GST tag N-Terminus

Applications

WB, ELISA

applications

Biologically active

No

Accession

Q15800

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 0.79% Tris HCl, 0.31% Glutathione

storage-buffer

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Complementary Products

Primary Antibodies

AB203587

Anti-C-4 Methylsterol Oxidase/SC4MOL antibody

Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-C-4 Methylsterol Oxidase/SC4MOL antibody (AB203587)

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Cell Lines & Lysates

AB265787

Human SQLE (Squalene Epoxidase) knockout HeLa cell line

Sanger Sequencing - Human SQLE (Squalene Epoxidase) knockout HeLa cell line (AB265787)

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  • 0
Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

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  • 20
Proteins & Peptides

AB130046

Recombinant Human MEMO1 protein (His tag N-Terminus)

SDS-PAGE - Recombinant Human MEMO1 protein (His tag N-Terminus) (AB130046)

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  • 0
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "ELISA": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as C-4 Methylsterol Oxidase.
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Sequence info

[{"sequence":"MATNESVSIFSSASLAVEYVDSLLPENPLQEPFKNAWNYMLNNYTKFQIATWGSLIVHEALYFLFCLPGFLFQFIPYMKKYKIQKDKPETWENQWKCFKVLLFNHFCIQLPLICGTYYFTEYFNIPYDWERMPRWYFLLARCFGCAVIEDTWHYFLHRLLHHKRIYKYIHKVHHEFQAPFGMEAEYAHPLETLILGTGFFIGIVLLCDHVILLWAWVTIRLLETIDVHSGYDIPLNPLNLIPFYAGSRHHDFHHMNFIGNYASTFTWWDRIFGTDSQYNAYNEKRKKFEKKTE","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":293,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Wheat germ","accessionNumber":"Q15800","tags":[{"tag":"GST","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-4 Methylsterol Oxidase also known as SC4MOL is an important enzyme involved in cholesterol biosynthesis. This enzyme is responsible for the demethylation of 44-dimethylsterols in the cholesterol synthesis process. It is a protein with a molecular mass of approximately 47 kDa. SC4MOL is widely expressed across various tissues including the liver where it has a significant role in lipid metabolism.
Biological function summary

The role of C-4 Methylsterol Oxidase is important for the efficient production of cholesterol which is a component of cell membranes and precursor for steroid hormones. This enzyme belongs to the sterol C4-methyl oxidase complex contributing to the fine-tuning of sterol intermediates during their conversion to cholesterol. Its activity ensures the reduction of certain sterol precursors and the stability of membrane fluidity across diverse physiological conditions.

Pathways

The enzyme is involved in the Kandutsch-Russell pathway and the Bloch pathway both of which are essential for cholesterol biosynthesis. These pathways manage the flow of sterol intermediates toward cholesterol while maintaining cellular homeostasis. SC4MOL interacts with other important enzymes like HMG-CoA reductase and lanosterol synthase playing a critical role in these metabolic processes.

SC4MOL has links to conditions such as Conradi–Hünermann syndrome and multiple sclerosis. Mutations or deficiencies in SC4MOL may result in abnormal sterol accumulation leading to these disorders. In Conradi–Hünermann syndrome the pathway disruption caused by SC4MOL impacts the processing of specific sterols while in multiple sclerosis cholesterol biosynthesis abnormalities can influence myelin sheath integrity associating the enzyme with this neurological condition through its interaction with other cholesterol-related proteins.
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Specifications

Form

Liquid

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General info

Function

Catalyzes the three-step monooxygenation required for the demethylation of 4,4-dimethyl and 4alpha-methylsterols, which can be subsequently metabolized to cholesterol (PubMed : 21285510, PubMed : 23583456, PubMed : 26114596, PubMed : 28673550, PubMed : 36958722). Also involved in drug metabolism, as it can metabolize eldecalcitol (ED-71 or 1alpha,25-dihydroxy-2beta-(3-hydroxypropoxy)-cholecalciferol), a second-generation vitamin D analog, into 1alpha,2beta,25-trihydroxy vitamin D3; this reaction occurs via enzymatic hydroxylation and spontaneous O-dehydroxypropylation (PubMed : 26038696).

Sequence similarities

Belongs to the sterol desaturase family.

Post-translational modifications

Ubiquitinated by MARCHF6, leading to proteasomal degradation.

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Product protocols

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Target data

Catalyzes the three-step monooxygenation required for the demethylation of 4,4-dimethyl and 4alpha-methylsterols, which can be subsequently metabolized to cholesterol (PubMed : 21285510, PubMed : 23583456, PubMed : 26114596, PubMed : 28673550, PubMed : 36958722). Also involved in drug metabolism, as it can metabolize eldecalcitol (ED-71 or 1alpha,25-dihydroxy-2beta-(3-hydroxypropoxy)-cholecalciferol), a second-generation vitamin D analog, into 1alpha,2beta,25-trihydroxy vitamin D3; this reaction occurs via enzymatic hydroxylation and spontaneous O-dehydroxypropylation (PubMed : 26038696).
See full target information MSMO1
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