Recombinant human c-Kit (mutated D820E) protein is a Human Fragment protein, in the 544 to 976 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >85% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
T Y K Y L Q K P M Y E V Q W K V V E E I N G N N Y V Y I D P T Q L P Y D H K W E F P R N R L S F G K T L G A G A F G K V V E A T A Y G L I K S D A A M T V A V K M L K P S A H L T E R E A L M S E L K V L S Y L G N H M N I V N L L G A C T I G G P T L V I T E Y C C Y G D L L N F L R R K R D S F I C S K Q E D H A E A A L Y K N L L H S K E S S C S D S T N E Y M D M K P G V S Y V V P T K A D K R R S V R I G S Y I E R D V T P A I M E D D E L A L D L E D L L S F S Y Q V A K G M A F L A S K N C I H R D L A A R N I L L T H G R I T K I C D F G L A R D I K N E S N Y V V K G N A R L P V K W M A P E S I F N C V Y T F E S D V W S Y G I F L W E L F S L G S S P Y P G M P V D S K F Y K M I K E G F R M L S P E H A P A E M Y D I M K T C W D A D P L K R P T F K Q I V Q L I E K Q I S E S T N H I Y S N L A N C S P N R Q K P V V D H S V R I N S V G S T A S S S Q P L L V H D D V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
Alternative names
CD117, SCFR, KIT, Mast/stem cell growth factor receptor Kit, Piebald trait protein, Proto-oncogene c-Kit, Tyrosine-protein kinase Kit, p145 c-kit, v-kit Hardy-Zuckerman 4 feline sarcoma viral oncogene homolog, PBT
Recommended products
Recombinant human c-Kit (mutated D820E) protein is a Human Fragment protein, in the 544 to 976 aa range, expressed in Baculovirus infected Sf9, with >85% purity and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >85% Densitometry
- Expression system
- Baculovirus infected Sf9 cells
- Tags
- GST tag N-Terminus
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- The specific activity of ab184893 was determined to be 16 nmol/min/mg.
- Accession
- P10721-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 25% Glycerol (glycerin, glycerine), 0.88% Sodium chloride, 0.79% Tris HCl, 0.31% Glutathione, 0.004% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.003% EDTA, 0.002% PMSF
Sequence info
Amino acid sequence
- T Y K Y L Q K P M Y E V Q W K V V E E I N G N N Y V Y I D P T Q L P Y D H K W E F P R N R L S F G K T L G A G A F G K V V E A T A Y G L I K S D A A M T V A V K M L K P S A H L T E R E A L M S E L K V L S Y L G N H M N I V N L L G A C T I G G P T L V I T E Y C C Y G D L L N F L R R K R D S F I C S K Q E D H A E A A L Y K N L L H S K E S S C S D S T N E Y M D M K P G V S Y V V P T K A D K R R S V R I G S Y I E R D V T P A I M E D D E L A L D L E D L L S F S Y Q V A K G M A F L A S K N C I H R D L A A R N I L L T H G R I T K I C D F G L A R D I K N E S N Y V V K G N A R L P V K W M A P E S I F N C V Y T F E S D V W S Y G I F L W E L F S L G S S P Y P G M P V D S K F Y K M I K E G F R M L S P E H A P A E M Y D I M K T C W D A D P L K R P T F K Q I V Q L I E K Q I S E S T N H I Y S N L A N C S P N R Q K P V V D H S V R I N S V G S T A S S S Q P L L V H D D V
- Accession
- P10721
- Protein length
- Fragment
- Predicted molecular weight
- 73 kDa
- Amino acids
- 544 to 976
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Affinity purified.
General info
- Function
Tyrosine-protein kinase that acts as a cell-surface receptor for the cytokine KITLG/SCF and plays an essential role in the regulation of cell survival and proliferation, hematopoiesis, stem cell maintenance, gametogenesis, mast cell development, migration and function, and in melanogenesis. In response to KITLG/SCF binding, KIT can activate several signaling pathways. Phosphorylates PIK3R1, PLCG1, SH2B2/APS and CBL. Activates the AKT1 signaling pathway by phosphorylation of PIK3R1, the regulatory subunit of phosphatidylinositol 3-kinase. Activated KIT also transmits signals via GRB2 and activation of RAS, RAF1 and the MAP kinases MAPK1/ERK2 and/or MAPK3/ERK1. Promotes activation of STAT family members STAT1, STAT3, STAT5A and STAT5B. Activation of PLCG1 leads to the production of the cellular signaling molecules diacylglycerol and inositol 1,4,5-trisphosphate. KIT signaling is modulated by protein phosphatases, and by rapid internalization and degradation of the receptor. Activated KIT promotes phosphorylation of the protein phosphatases PTPN6/SHP-1 and PTPRU, and of the transcription factors STAT1, STAT3, STAT5A and STAT5B. Promotes phosphorylation of PIK3R1, CBL, CRK (isoform Crk-II), LYN, MAPK1/ERK2 and/or MAPK3/ERK1, PLCG1, SRC and SHC1.
- Sequence similarities
Belongs to the protein kinase superfamily. Tyr protein kinase family. CSF-1/PDGF receptor subfamily.
- Post-translational modifications
Ubiquitinated by SOCS6. KIT is rapidly ubiquitinated after autophosphorylation induced by KITLG/SCF binding, leading to internalization and degradation.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Notes
It is recommended to store this protein at -80°C in glycerol to preserve the activity of the protein.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The c-Kit protein also known as CD117 is a type of receptor tyrosine kinase with a molecular weight of approximately 145 kDa. It is encoded by the KIT gene and commonly expressed on the surface of hematopoietic stem cells mast cells melanocytes and germ cells. C-Kit plays a role in the signal transduction pathways that control cell proliferation and survival. Researchers often identify c-Kit using markers like biotin c-Kit PerCP Cy5.5 or antibodies such as anti-c-Kit for experimental purposes.
- Biological function summary
C-Kit influences the function and survival of certain cell types. When it binds to its ligand stem cell factor (SCF) it activates receptor dimerization and phosphorylation triggering downstream signaling. C-Kit does not act alone; it often functions in a complex with other proteins to affect cellular outcomes. Its expression is vital for the maintenance of bone marrow hematopoietic cells and contributes to melanocyte and germ cell development.
- Pathways
C-Kit interacts with the PI3K/AKT and RAS/ERK pathways through its kinase activity. These pathways play important roles in cell division differentiation and survival. The c-Abl and ACK2 proteins are closely related to c-Kit function within these signaling networks contributing to regulating cellular responses to external stimuli.
- Associated diseases and disorders
C-Kit mutations are frequently associated with gastrointestinal stromal tumors (GIST) and acute myeloid leukemia (AML). These conditions often involve alterations in c-Kit activity leading to uncontrolled cell growth. While studying GIST researchers have noticed interactions between c-Kit and other proteins like CBL which is involved in the modulation of c-Kit signaling. Understanding these interactions helps in designing targeted therapies for conditions involving c-Kit dysregulation.
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4 product images
Functional Studies - Recombinant human c-Kit (mutated D820E) protein (ab184893)
The specific activity of c-Kit (ab184893) was determined to be 9 nmol/min/mg as per activity assay protocol and was equivalent to 16 nmol/min/mg as per radiometric assay
SDS-PAGE - Recombinant human c-Kit (mutated D820E) protein (ab184893)
SDS PAGE analysis of ab184893
SDS-PAGE - Recombinant human c-Kit (mutated D820E) protein (ab184893)
SDS-PAGE analysis of ab184893.
Functional Studies - Recombinant human c-Kit (mutated D820E) protein (ab184893)
Kinase Assay determining the specific activity of ab184893 to be 16 nmol/min/mg.
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