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Recombinant human c-Myc (mutated T73A) protein is a Human Full Length protein, in the 1 to 454 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.

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Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, FuncS

Biologically active

Yes

Amino acid sequence

M D F F R V V E N Q Q P P A T M P L N V S F T N R N Y D L D Y D S V Q P Y F Y C D E E E N F Y Q Q Q Q Q S E L Q P P A P S E D I W K K F E L L P A P P L S P S R R S G L C S P S Y V A V T P F S L R G D N D G G G G S F S T A D Q L E M V T E L L G G D M V N Q S F I C D P D D E T F I K N I I I Q D C M W S G F S A A A K L V S E K L A S Y Q A A R K D S G S P N P A R G H S V C S T S S L Y L Q D L S A A A S E C I D P S V V F P Y P L N D S S S P K S C A S Q D S S A F S P S S D S L L S S T E S S P Q G S P E P L V L H E E T P P T T S S D S E E E Q E D E E E I D V V S V E K R Q A P G K R S E S G S P S A G G H S K P P H S P L V L K R C H V S T H Q H N Y A A P P S T R K D Y P A A K R V K L D S V R V L R Q I S N N R K C T S P R S S D T E E N V K R R T H N V L E R Q R R N E L K R S F F A L R D Q I P E L E N N E K A P K V V I L K K A T A Y I L S V Q A E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A E S G G G G S P G R R R R R R R R R R R

Reactivity data

Application

SDS-PAGE

Reactivity

Reacts

Dilution info

-

Notes

-

Application

FuncS

Reactivity

Reacts

Dilution info

-

Notes

-

Associated Products

Select an associated product type

4 products for Alternative Product

Target data

Function

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029). Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).

Alternative names

Recommended products

Recombinant human c-Myc (mutated T73A) protein is a Human Full Length protein, in the 1 to 454 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, FuncS.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Applications

SDS-PAGE, FuncS

Biological activity

DNA binding activity was demonstrated using cMyc specific DNA binding oligo derived ELISA. Mouse iPS generation activity was measured for each lot of products.

Accession

P01106

Animal free

No

Species

Human

Concentration
Loading...
Storage buffer

pH: 8
Constituents: Glycerol (glycerin, glycerine), Potassium chloride, Sodium chloride, 3.4% DL-Arginine, 0.32% Tris HCl, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.02% EDTA

Sequence info

Amino acid sequence

M D F F R V V E N Q Q P P A T M P L N V S F T N R N Y D L D Y D S V Q P Y F Y C D E E E N F Y Q Q Q Q Q S E L Q P P A P S E D I W K K F E L L P A P P L S P S R R S G L C S P S Y V A V T P F S L R G D N D G G G G S F S T A D Q L E M V T E L L G G D M V N Q S F I C D P D D E T F I K N I I I Q D C M W S G F S A A A K L V S E K L A S Y Q A A R K D S G S P N P A R G H S V C S T S S L Y L Q D L S A A A S E C I D P S V V F P Y P L N D S S S P K S C A S Q D S S A F S P S S D S L L S S T E S S P Q G S P E P L V L H E E T P P T T S S D S E E E Q E D E E E I D V V S V E K R Q A P G K R S E S G S P S A G G H S K P P H S P L V L K R C H V S T H Q H N Y A A P P S T R K D Y P A A K R V K L D S V R V L R Q I S N N R K C T S P R S S D T E E N V K R R T H N V L E R Q R R N E L K R S F F A L R D Q I P E L E N N E K A P K V V I L K K A T A Y I L S V Q A E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A E S G G G G S P G R R R R R R R R R R R

Accession

P01106

Protein length

Full Length

Predicted molecular weight

52.95 kDa

Amino acids

1 to 454

Nature

Recombinant

Specifications

Form

Liquid

Additional notes

ab135024 was expressed in E. coli as inclusion bodies, refolded using temperature shift inclusion body refolding technology and chromatographically purified.

General info

Function

Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029). Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).

Post-translational modifications

Phosphorylated by PRKDC (PubMed:1597196). Phosphorylation at Ser-344 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-77 by CDK2 prevents Ras-induced senescence (PubMed:19966300, PubMed:20713526). Phosphorylated at Ser-77 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-73 (PubMed:22307329). Phosphorylation at Thr-73 and Ser-77 by GSK3 is required for ubiquitination and degradation by the proteasome (PubMed:15103331, PubMed:17558397, PubMed:8386367). Dephosphorylation at Ser-77 by protein phosphatase 2A (PPP2CA) promotes its degradation; interaction with PPP2CA is enhanced by AMBRA1 (PubMed:25438055, PubMed:25803737).

Subcellular localisation

Nucleus, Nucleoplasm, Nucleolus

Storage

Shipped at conditions

Blue Ice

Appropriate short-term storage conditions

-20°C

Appropriate long-term storage conditions

-20°C

Aliquoting information

Upon delivery aliquot

Storage information

Avoid freeze / thaw cycle

This product is an active protein and may elicit a biological response in vivo, handle with caution.

Notes

Incubating ab135024 in culture mediums at concentration of 8 µg/ml may be used for protein derived iPS (PiPS) generation when combined with Oct4-11R, Sox2-11R and Klf4-11R.

Supplementary info

This supplementary information is collated from multiple sources and compiled automatically.

Activity summary

C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.

Biological function summary

C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.

Pathways

C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.

Associated diseases and disorders

C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.

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