Recombinant human c-Myc protein (Active) is a Human Full Length protein, in the 1 to 454 aa range, expressed in Escherichia coli, with >93% purity, 5 EU/µg endotoxin level and suitable for western blot, SDS-PAGE, Functional studies and mass spectrometry. The predicted molecular weight of ab169901 protein is 53 kDa.
- Save time and ensure accurate results - use our recombinant c-Myc protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Cited in over 10 publications
M D F F R V V E N Q Q P P A T M P L N V S F T N R N Y D L D Y D S V Q P Y F Y C D E E E N F Y Q Q Q Q Q S E L Q P P A P S E D I W K K F E L L P T P P L S P S R R S G L C S P S Y V A V T P F S L R G D N D G G G G S F S T A D Q L E M V T E L L G G D M V N Q S F I C D P D D E T F I K N I I I Q D C M W S G F S A A A K L V S E K L A S Y Q A A R K D S G S P N P A R G H S V C S T S S L Y L Q D L S A A A S E C I D P S V V F P Y P L N D S S S P K S C A S Q D S S A F S P S S D S L L S S T E S S P Q G S P E P L V L H E E T P P T T S S D S E E E Q E D E E E I D V V S V E K R Q A P G K R S E S G S P S A G G H S K P P H S P L V L K R C H V S T H Q H N Y A A P P S T R K D Y P A A K R V K L D S V R V L R Q I S N N R K C T S P R S S D T E E N V K R R T H N V L E R Q R R N E L K R S F F A L R D Q I P E L E N N E K A P K V V I L K K A T A Y I L S V Q A E E Q K L I S E E D L L R K R R E Q L K H K L E Q L R N S C A E S G G G G S P G R R R R R R R R R R R
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Select an associated product type
Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029). Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).
BHLHE39, MYC, Myc proto-oncogene protein, Class E basic helix-loop-helix protein 39, Proto-oncogene c-Myc, Transcription factor p64, bHLHe39
Recombinant human c-Myc protein (Active) is a Human Full Length protein, in the 1 to 454 aa range, expressed in Escherichia coli, with >93% purity, 5 EU/µg endotoxin level and suitable for western blot, SDS-PAGE, Functional studies and mass spectrometry. The predicted molecular weight of ab169901 protein is 53 kDa.
- Save time and ensure accurate results - use our recombinant c-Myc protein as a control
- Optimal protein bioactivity, stability and reproducibility
- Cited in over 10 publications
Intracellular protein penetration rate was tested using DyLight labeled ab169901 protein at 1 μg/ ml for 30 min incubation for human fibroblast cells at 37oC. More than 90% cell will be positive one hour after sample incubation.
pH: 7.5
Constituents: 0.24% Tris
ab169901 was expressed in E. coli as inclusion bodies, solubilized, refolded, and further purified.
Transcription factor that binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3' (PubMed:24940000, PubMed:25956029). Activates the transcription of growth-related genes (PubMed:24940000, PubMed:25956029). Binds to the VEGFA promoter, promoting VEGFA production and subsequent sprouting angiogenesis (PubMed:24940000, PubMed:25956029). Regulator of somatic reprogramming, controls self-renewal of embryonic stem cells (By similarity). Functions with TAF6L to activate target gene expression through RNA polymerase II pause release (By similarity). Positively regulates transcription of HNRNPA1, HNRNPA2 and PTBP1 which in turn regulate splicing of pyruvate kinase PKM by binding repressively to sequences flanking PKM exon 9, inhibiting exon 9 inclusion and resulting in exon 10 inclusion and production of the PKM M2 isoform (PubMed:20010808).
Phosphorylated by PRKDC (PubMed:1597196). Phosphorylation at Ser-344 by PIM2 leads to the stabilization of MYC (By similarity). Phosphorylation at Ser-77 by CDK2 prevents Ras-induced senescence (PubMed:19966300, PubMed:20713526). Phosphorylated at Ser-77 by DYRK2; this primes the protein for subsequent phosphorylation by GSK3B at Thr-73 (PubMed:22307329). Phosphorylation at Thr-73 and Ser-77 by GSK3 is required for ubiquitination and degradation by the proteasome (PubMed:15103331, PubMed:17558397, PubMed:8386367). Dephosphorylation at Ser-77 by protein phosphatase 2A (PPP2CA) promotes its degradation; interaction with PPP2CA is enhanced by AMBRA1 (PubMed:25438055, PubMed:25803737).
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Ensure the validity of your result using our bioactive recombinant human c-Myc protein ab169901 as a positive control in western blot and SDS-PAGE.
ab169901 is fused to an eleven arginine (11R) membrane penetration domain at the C terminus to enable penetration across the plasma membrane of mammalian cells.
Cellular Toxicity: The ab169901 recombinant protein was tested on mouse embryonic stem cells up to 50 µg/mL in culture medium. Suggested reprogramming protein concentration is between 0.5 to 8 µg/mL for both human and mouse fibroblast cells applications.
Check out our protein gel staining guide for SDS-PAGE here
Check out of western blot protocol for more information here
C-Myc also known simply as Myc or MYC protein is a transcription factor with significant roles in cellular processes. Its estimated molecular weight is approximately 62 kDa. This protein is expressed in various tissues and cell types including common use in HEK (human embryonic kidney) cells for research. c-Myc functions by regulating transcription of genes involved in cell cycle progression apoptosis and cellular transformation.
C-Myc is involved in regulating cell growth and proliferation. It forms part of the Myc/Max complex which binds to DNA to regulate gene expression. This activity affects cellular metabolism ribosome biogenesis and cell cycle entry emphasizing its regulation of cellular energy and stress response. Its expression levels critically govern normal cellular functions and homeostasis.
C-Myc plays an important role in the cell cycle pathway and apoptosis regulation. Specifically c-Myc is associated with the Wnt signaling pathway which influences cellular proliferation and differentiation. It also interacts with other proteins like Cyclin D1 influencing cell cycle control. These interactions ensure c-Myc's involvement in regulating key processes related to cell proliferation and stability.
C-Myc is tightly linked to cancer such as Burkitt's lymphoma and colon cancer. In these conditions c-Myc overexpression contributes to uncontrolled cell proliferation. Additionally c-Myc is associated with other oncogenic proteins like BCL2 in tumorigenesis highlighting its pivotal role in cancer development and progression. Understanding c-Myc's involvement in these diseases aids in the development of targeted therapeutic strategies.
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com