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AB51784

Recombinant Human C Reactive Protein

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Recombinant Human C Reactive Protein is a Human Full Length protein, in the 1 to 224 aa range, expressed in Escherichia coli, with >95%, suitable for SDS-PAGE, WB.

View Alternative Names

PTX1, CRP, C-reactive protein

2 Images
Western blot - Recombinant Human C Reactive Protein (AB51784)
  • WB

Unknown

Western blot - Recombinant Human C Reactive Protein (AB51784)

All lanes:

Western blot - Anti-C Reactive Protein antibody (<a href='/en-us/products/primary-antibodies/c-reactive-protein-antibody-ab78051'>ab78051</a>) at 1 µg/mL

All lanes:

Western blot - Recombinant Human C Reactive Protein (ab51784) at 0.01 µg

Secondary

All lanes:

Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (<a href='/en-us/products/secondary-antibodies/goat-rabbit-igg-h-l-hrp-preadsorbed-ab97080'>ab97080</a>) at 1/5000 dilution

Predicted band size: 25 kDa

false

Exposure time: 1min

SDS-PAGE - Recombinant Human C Reactive Protein (AB51784)
  • SDS-PAGE

Supplier Data

SDS-PAGE - Recombinant Human C Reactive Protein (AB51784)

4-20% SDS-PAGE of Human CRP : 5 ug of Recombinant human CRP (C-reactive protein) loaded in each lane under reducing conditions and stained with Coomassie Blue. Recombinant human CRP has a predicted MW of ~ 115 kDa corresponding to the pentameric structure of 23 kDa monomers.

Key facts

Purity

>95% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE, WB

applications

Biologically active

No

Accession

P02741

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Preservative: 0.05% Sodium azide Constituents: 0.81% Sodium chloride, 0.24% Tris, 0.02% Calcium chloride

storage-buffer

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Complementary Products

Secondary Antibodies

AB150077

Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (AB150077)

  • 5
  • 20
Primary Antibodies

AB9485

Anti-GAPDH antibody - Loading Control

BOND RX™ Validated|Lab Essentials

Immunocytochemistry/ Immunofluorescence - Anti-GAPDH antibody - Loading Control (AB9485)

  • 5
  • 88
Primary Antibodies

AB307519

Alexa Fluor® 647 Anti-C Reactive Protein antibody [Y284]

BOND RX™ Validated|RabMAb|Recombinant

Immunohistochemistry - Alexa Fluor® 647 Anti-C Reactive Protein antibody [Y284] (AB307519)

  • 0
  • 0
Primary Antibodies

AB220163

Anti-YTHDF2 antibody [EPR20318]

RabMAb|Recombinant|KO Validated

Western blot - Anti-YTHDF2 antibody [EPR20318] (AB220163)

  • 5
  • 3
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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "WB": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p>ab51784 can be used as a WB positive control in conjunction with <a href='/en-us/products/primary-antibodies/c-reactive-protein-antibody-ab78051'>ab78051</a>.</p>" } } }
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Product details

This product is manufactured by BioVision, an Abcam company and was previously called 4863 CRP, human recombinant. 4863-10 is the same size as the 10 μg size of ab51784.

Endotoxin level is <0.1 ng/μg.

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Sequence info

[{"sequence":"AA Sequence: MEKLLCFLVL TSLSHAFGQT DMSRKAFVFP KESDTSYVSL KAPLTKPLKA\r\nFTVCLHFYTE LSSTRGYSIF SYATKRQDNE ILIFWSKDIG YSFTVGGSEI LFEVPEVTVA\r\nPVHICTSWES ASGIVEFWVD GKPRVRKSLK KGYTVGAEAS IILGQEQDSF GGNFEGSQSL\r\nVGDIGNVNMW DFVLSPDEIN TIYLGGPFSP NVLNWRALKY EVQGEVFTKP QLWP","proteinLength":"Full Length","predictedMolecularWeight":"115 kDa","actualMolecularWeight":null,"aminoAcidEnd":224,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P02741","tags":[]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate long-term storage conditions
-20°C
Storage information
Avoid freeze / thaw cycle|For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA)|Reconstitute for long term storage
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C-reactive protein (CRP) also known as CRP protein is an acute-phase protein with a molecular mass of approximately 25 kDa. It is primarily synthesized in the liver in response to cytokines. This protein appears in the blood where it binds to phosphocholine expressed on the surface of dead or dying cells. CRP is often used as a biomarker for inflammation in the body and its levels in human and canine (anti CRP used) blood samples are measured using assays like the CRP ELISA test or CRP ELISA kit.
Biological function summary

CRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.

Pathways

CRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.

Elevated CRP levels are commonly associated with cardiovascular diseases and rheumatoid arthritis. In cardiovascular diseases CRP acts as a marker for atherosclerosis where the protein levels correlate with disease severity. It connects with other proteins involved in inflammatory processes such as fibrinogen to contribute to disease pathology. In rheumatoid arthritis CRP provides insights into disease activity helping in the assessment of treatment responses.
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Specifications

Form

Liquid

Additional notes

>95% by SDS-PAGE, HPLC analysis and anion exchange FPLC. Recombinant Human CRP is purified by proprietary chromatographic techniques.

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General info

Function

Displays several functions associated with host defense : it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Sequence similarities

Belongs to the pentraxin family.

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Product protocols

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Target data

Displays several functions associated with host defense : it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
See full target information CRP
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Product promise

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