Recombinant Human C Reactive Protein is a Human Full Length protein, in the 1 to 224 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
A A S e q u e n c e : M E K L L C F L V L T S L S H A F G Q T D M S R K A F V F P K E S D T S Y V S L K A P L T K P L K A F T V C L H F Y T E L S S T R G Y S I F S Y A T K R Q D N E I L I F W S K D I G Y S F T V G G S E I L F E V P E V T V A P V H I C T S W E S A S G I V E F W V D G K P R V R K S L K K G Y T V G A E A S I I L G Q E Q D S F G G N F E G S Q S L V G D I G N V N M W D F V L S P D E I N T I Y L G G P F S P N V L N W R A L K Y E V Q G E V F T K P Q L W P
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab51784 can be used as a WB positive control in conjunction with Anti-C Reactive Protein antibody ab78051. |
Target data
Function
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
Alternative names
PTX1, CRP, C-reactive protein
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Recombinant Human C Reactive Protein is a Human Full Length protein, in the 1 to 224 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- P02741-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Preservative: 0.05% Sodium azide
Constituents: 0.81% Sodium chloride, 0.24% Tris, 0.02% Calcium chloride
Sequence info
Amino acid sequence
- A A S e q u e n c e : M E K L L C F L V L T S L S H A F G Q T D M S R K A F V F P K E S D T S Y V S L K A P L T K P L K A F T V C L H F Y T E L S S T R G Y S I F S Y A T K R Q D N E I L I F W S K D I G Y S F T V G G S E I L F E V P E V T V A P V H I C T S W E S A S G I V E F W V D G K P R V R K S L K K G Y T V G A E A S I I L G Q E Q D S F G G N F E G S Q S L V G D I G N V N M W D F V L S P D E I N T I Y L G G P F S P N V L N W R A L K Y E V Q G E V F T K P Q L W P
- Accession
- P02741
- Protein length
- Full Length
- Predicted molecular weight
- 115 kDa
- Amino acids
- 1 to 224
- Nature
- Recombinant
Specifications
- Form
- Liquid
- Additional notes
>95% by SDS-PAGE, HPLC analysis and anion exchange FPLC. Recombinant Human CRP is purified by proprietary chromatographic techniques.
General info
- Function
Displays several functions associated with host defense: it promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. Can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.
- Sequence similarities
Belongs to the pentraxin family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate long-term storage conditions
- -20°C
- Storage information
- Avoid freeze / thaw cycle, For long term storage it is recommended to add a carrier protein on reconstitution (0.1% HSA or BSA), Reconstitute for long term storage
Notes
This product is manufactured by BioVision, an Abcam company and was previously called 4863 CRP, human recombinant. 4863-10 is the same size as the 10 μg size of ab51784.
Endotoxin level is <0.1 ng/μg.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C-reactive protein (CRP) also known as CRP protein is an acute-phase protein with a molecular mass of approximately 25 kDa. It is primarily synthesized in the liver in response to cytokines. This protein appears in the blood where it binds to phosphocholine expressed on the surface of dead or dying cells. CRP is often used as a biomarker for inflammation in the body and its levels in human and canine (anti CRP used) blood samples are measured using assays like the CRP ELISA test or CRP ELISA kit.
- Biological function summary
CRP plays a significant role in the innate immune system by recognizing foreign pathogens and damaged cells. It does not form part of a larger complex but itself can bind to specific ligands initiating the complement cascade. This activation enhances phagocytosis by macrophages contributing to the clearance of necrotic and apoptotic cells. The CRP protein therefore acts as a fast responder in the presence of inflammation making it a useful indicator for monitoring inflammatory diseases.
- Pathways
CRP operates predominantly within the acute-phase response pathway. Its production is upregulated by cytokines such as IL-6 which connects it to inflammation-signaling pathways. CRP also interacts with the complement system bridging innate and adaptive immunity. Besides CRP could indirectly influence pathways involving other inflammatory proteins like serum amyloid A and haptoglobin creating a network of responses important for managing inflammation.
- Associated diseases and disorders
Elevated CRP levels are commonly associated with cardiovascular diseases and rheumatoid arthritis. In cardiovascular diseases CRP acts as a marker for atherosclerosis where the protein levels correlate with disease severity. It connects with other proteins involved in inflammatory processes such as fibrinogen to contribute to disease pathology. In rheumatoid arthritis CRP provides insights into disease activity helping in the assessment of treatment responses.
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2 product images
Western blot - Recombinant Human C Reactive Protein (ab51784)
All lanes: Western blot - Anti-C Reactive Protein antibody (Anti-C Reactive Protein antibody ab78051) at 1 µg/mL
All lanes: Western blot - Recombinant Human C Reactive Protein (ab51784) at 0.01 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Performed under reducing conditions.
Predicted band size: 25 kDa
Exposure time: 1min
SDS-PAGE - Recombinant Human C Reactive Protein (ab51784)
4-20% SDS-PAGE of Human CRP: 5 ug of Recombinant human CRP (C-reactive protein) loaded in each lane under reducing conditions and stained with Coomassie Blue. Recombinant human CRP has a predicted MW of ~ 115 kDa corresponding to the pentameric structure of 23 kDa monomers.
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