Recombinant Human C11B2/CYP11B2 protein (His tag) is a Human Full Length protein, in the 25 to 503 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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- Acta physiologica (Oxford, England) 237:e138992022Collecting duct renin regulates potassium homeostasis in mice.Applications:Unspecified applicationReactive species:Unspecified reactive speciesChuanming Xu,Yanting Chen,Nirupama Ramkumar,Chang-Jiang Zou,Curt D Sigmund,Tianxin YangPubMed 36264268
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
G T R A A R A P R T V L P F E A M P Q H P G N R W L R L L Q I W R E Q G Y E H L H L E M H Q T F Q E L G P I F R Y N L G G P R M V C V M L P E D V E K L Q Q V D S L H P C R M I L E P W V A Y R Q H R G H K C G V F L L N G P E W R F N R L R L N P D V L S P K A V Q R F L P M V D A V A R D F S Q A L K K K V L Q N A R G S L T L D V Q P S I F H Y T I E A S N L A L F G E R L G L V G H S P S S A S L N F L H A L E V M F K S T V Q L M F M P R S L S R W I S P K V W K E H F E A W D C I F Q Y G D N C I Q K I Y Q E L A F N R P Q H Y T G I V A E L L L K A E L S L E A I K A N S M E L T A G S V D T T A F P L L M T L F E L A R N P D V Q Q I L R Q E S L A A A A S I S E H P Q K A T T E L P L L R A A L K E T L R L Y P V G L F L E R V V S S D L V L Q N Y H I P A G T L V Q V F L Y S L G R N A A L F P R P E R Y N P Q R W L D I R G S G R N F H H V P F G F G M R Q C L G R R L A E A E M L L L L H H V L K H F L V E T L T Q E D I K M V Y S F I L R P G T S P L L T F R A I N
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
A cytochrome P450 monooxygenase that catalyzes the biosynthesis of aldosterone, the main mineralocorticoid in the human body responsible for salt and water homeostasis, thus involved in blood pressure regulation, arterial hypertension, and the development of heart failure (PubMed:11856349, PubMed:12530636, PubMed:1518866, PubMed:15356073, PubMed:1594605, PubMed:1775135, PubMed:22446688, PubMed:23322723, PubMed:9814482, PubMed:9814506). Catalyzes three sequential oxidative reactions of 11-deoxycorticosterone (21-hydroxyprogesterone), namely 11-beta hydroxylation, followed by two successive oxidations at C18 yielding 18-hydroxy and then 18-oxo intermediates (that would not leave the enzyme active site during the consecutive hydroxylation reactions), ending with the formation of aldosterone (PubMed:11856349, PubMed:12530636, PubMed:1518866, PubMed:1594605, PubMed:1775135, PubMed:22446688, PubMed:23322723, PubMed:9814506). Can also produce 18-hydroxycortisol and 18-oxocortisol, derived from successive oxidations of cortisol at C18, normally found at very low levels, but significantly increased in primary aldosteronism, the most common form of secondary hypertension (PubMed:15356073, PubMed:9814482). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed:11856349, PubMed:1594605, PubMed:23322723, PubMed:9814506). Could also be involved in the androgen metabolic pathway (Probable).
Alternative names
Aldosterone synthase, Aldosterone-synthesizing enzyme, CYPXIB2, Cytochrome P-450Aldo, Cytochrome P-450C18, Steroid 18-hydroxylase, ALDOS, CYP11B2
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Recombinant Human C11B2/CYP11B2 protein (His tag) is a Human Full Length protein, in the 25 to 503 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P19099-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.2 - 7.4
Constituents: Tris buffer, 50% Glycerol (glycerin, glycerine)
Sequence info
Amino acid sequence
- G T R A A R A P R T V L P F E A M P Q H P G N R W L R L L Q I W R E Q G Y E H L H L E M H Q T F Q E L G P I F R Y N L G G P R M V C V M L P E D V E K L Q Q V D S L H P C R M I L E P W V A Y R Q H R G H K C G V F L L N G P E W R F N R L R L N P D V L S P K A V Q R F L P M V D A V A R D F S Q A L K K K V L Q N A R G S L T L D V Q P S I F H Y T I E A S N L A L F G E R L G L V G H S P S S A S L N F L H A L E V M F K S T V Q L M F M P R S L S R W I S P K V W K E H F E A W D C I F Q Y G D N C I Q K I Y Q E L A F N R P Q H Y T G I V A E L L L K A E L S L E A I K A N S M E L T A G S V D T T A F P L L M T L F E L A R N P D V Q Q I L R Q E S L A A A A S I S E H P Q K A T T E L P L L R A A L K E T L R L Y P V G L F L E R V V S S D L V L Q N Y H I P A G T L V Q V F L Y S L G R N A A L F P R P E R Y N P Q R W L D I R G S G R N F H H V P F G F G M R Q C L G R R L A E A E M L L L L H H V L K H F L V E T L T Q E D I K M V Y S F I L R P G T S P L L T F R A I N
- Accession
- P19099
- Protein length
- Full Length
- Predicted molecular weight
- 71 kDa
- Amino acids
- 25 to 503
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
A cytochrome P450 monooxygenase that catalyzes the biosynthesis of aldosterone, the main mineralocorticoid in the human body responsible for salt and water homeostasis, thus involved in blood pressure regulation, arterial hypertension, and the development of heart failure (PubMed:11856349, PubMed:12530636, PubMed:1518866, PubMed:15356073, PubMed:1594605, PubMed:1775135, PubMed:22446688, PubMed:23322723, PubMed:9814482, PubMed:9814506). Catalyzes three sequential oxidative reactions of 11-deoxycorticosterone (21-hydroxyprogesterone), namely 11-beta hydroxylation, followed by two successive oxidations at C18 yielding 18-hydroxy and then 18-oxo intermediates (that would not leave the enzyme active site during the consecutive hydroxylation reactions), ending with the formation of aldosterone (PubMed:11856349, PubMed:12530636, PubMed:1518866, PubMed:1594605, PubMed:1775135, PubMed:22446688, PubMed:23322723, PubMed:9814506). Can also produce 18-hydroxycortisol and 18-oxocortisol, derived from successive oxidations of cortisol at C18, normally found at very low levels, but significantly increased in primary aldosteronism, the most common form of secondary hypertension (PubMed:15356073, PubMed:9814482). Mechanistically, uses molecular oxygen inserting one oxygen atom into a substrate and reducing the second into a water molecule. Two electrons are provided by NADPH via a two-protein mitochondrial transfer system comprising flavoprotein FDXR (adrenodoxin/ferredoxin reductase) and nonheme iron-sulfur protein FDX1 or FDX2 (adrenodoxin/ferredoxin) (PubMed:11856349, PubMed:1594605, PubMed:23322723, PubMed:9814506). Could also be involved in the androgen metabolic pathway (Probable).
- Sequence similarities
Belongs to the cytochrome P450 family.
- Subcellular localisation
- Mitochondrion inner membrane
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -20°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Notes
This product was previously labelled as C11B2
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C11B2 also known as CYP11B2 is a highly specific enzyme that plays an essential role in the biosynthesis of aldosterone. Also referred to as aldosterone synthase this cytochrome P450 family member is encoded by the CYP11B2 gene. It has a molecular mass of approximately 58 kDa. Its expression occurs mainly in the adrenal cortex particularly within the zona glomerulosa cells where it catalyzes the final steps in the production of aldosterone from cholesterol.
- Biological function summary
CYP11B2 converts 11-deoxycorticosterone to corticosterone which is then converted to aldosterone. This enzyme functions independently and is not part of any larger protein complexes. It undertakes its role in the mitochondria of adrenal gland cells where it serves as an important factor in the corticosteroid synthesis pathway. Its activity is regulated via several factors including angiotensin II and potassium ion concentration to maintain homeostatic balance within the body.
- Pathways
CYP11B2 is an integral part of the steroidogenic pathway specifically within the branch that leads to mineralocorticoid biosynthesis. This pathway involves the conversion of cholesterol to steroid hormones and is closely linked to the renin-angiotensin-aldosterone system (RAAS) which governs blood pressure and fluid balance. Besides its relationship with cholesterol side-chain cleavage enzyme (CYP11A1) CYP11B2 interacts with other proteins like 3-beta-hydroxysteroid dehydrogenase in facilitating steroidogenesis.
- Associated diseases and disorders
Abnormalities in CYP11B2 expression or function can lead to aldosterone-related conditions such as primary aldosteronism and Addison’s disease. Primary aldosteronism results from overproduction of aldosterone causing hypertension and electrolyte imbalances while its deficiency contributes to Addison's disease. These disorders can also involve connections with proteins like CYP17A1 which is another key player in steroid biosynthesis and whose disruptions can influence CYP11B2's activity or expression amplifying disease pathology.
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SDS-PAGE - Recombinant Human C11B2/CYP11B2 protein (His tag) (ab224858)
(Tris-Glycine gel) Discontinuous SDS-PAGE (reduced) analysis of ab224858 with 5% enrichment gel and 15% separation gel.
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