Recombinant Human C1QA protein is a Human Full Length protein, in the 23 to 245 aa range, expressed in Wheat germ and suitable for ELISA, WB.
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Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
Amino acid sequence
E D L C R A P D G K K G E A G R P G R R G R P G L K G E Q G E P G A P G I R T G I Q G L K G D Q G E P G P S G N P G K V G Y P G P S G P L G A R G I P G I K G T K G S P G N I K D Q P R P A F S A I R R N P P M G G N V V I F D T V I T N Q E E P Y Q N H S G R F V C T V P G Y Y Y F T F Q V L S Q W E I C L S I V S S S R G Q V R R S L G F C D T T N K G L F Q V V S G G M V L Q L Q Q G D Q V W V E K D P K K G H I Y Q G S E A D S V F S G F L I F P S A
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application ELISA | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes - |
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Target data
Function
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
Alternative names
Complement C1q subcomponent subunit A, C1QA
Recommended products
Recombinant Human C1QA protein is a Human Full Length protein, in the 23 to 245 aa range, expressed in Wheat germ and suitable for ELISA, WB.
Key facts
- Expression system
- Wheat germ
- Tags
- GST tag N-Terminus
- Applications
- ELISA, WB
- Biologically active
- No
- Accession
- P02745-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.79% Tris HCl, 0.31% Glutathione
Sequence info
Amino acid sequence
- E D L C R A P D G K K G E A G R P G R R G R P G L K G E Q G E P G A P G I R T G I Q G L K G D Q G E P G P S G N P G K V G Y P G P S G P L G A R G I P G I K G T K G S P G N I K D Q P R P A F S A I R R N P P M G G N V V I F D T V I T N Q E E P Y Q N H S G R F V C T V P G Y Y Y F T F Q V L S Q W E I C L S I V S S S R G Q V R R S L G F C D T T N K G L F Q V V S G G M V L Q L Q Q G D Q V W V E K D P K K G H I Y Q G S E A D S V F S G F L I F P S A
- Accession
- P02745
- Protein length
- Full Length
- Amino acids
- 23 to 245
- Nature
- Recombinant
- Tags
- GST tag N-Terminus
Specifications
- Form
- Liquid
General info
- Function
C1q associates with the proenzymes C1r and C1s to yield C1, the first component of the serum complement system. The collagen-like regions of C1q interact with the Ca(2+)-dependent C1r(2)C1s(2) proenzyme complex, and efficient activation of C1 takes place on interaction of the globular heads of C1q with the Fc regions of IgG or IgM antibody present in immune complexes.
- Post-translational modifications
O-linked glycans are assumed to be the Glc-Gal disaccharides typically found as secondary modifications of hydroxylated lysines in collagen-like domains.
Storage
- Shipped at conditions
- Dry Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
The C1QA protein is one part of the complement component 1q (C1q) molecule also called C1Q-A. This protein plays an important role in the immune system. The C1q complex consists of three polypeptide chains: C1QA C1QB and C1QC. C1QA alone has a mass of around 26 kDa. These proteins mainly express in immune cells like macrophages dendritic cells and B cells. It forms part of the extracellular matrix where it participates in immune functions.
- Biological function summary
C1QA interacts with pathogens and modified host cells to trigger the complement cascade. This starts the activation of the classical pathway which enhances immune responses. C1QA together with C1QB and C1QC binds to Fc regions of antibodies attached to antigens. This binding leads to the subsequent activation of C1r and C1s proteases facilitating opsonization and clearance of immune complexes and apoptotic cells.
- Pathways
The synthesis of C1QA plays an important role in the classical complement pathway and is part of the innate immunity. C1QA collaborates with proteins like C1QB and C1QC in this pathway. This pathway bridges innate and adaptive immunity by assisting in the removal of pathogens and cell debris. It also relates to the coagulation pathway where its activity can influence inflammation and tissue repair processes.
- Associated diseases and disorders
Impaired C1QA function links to conditions like systemic lupus erythematosus (SLE) and rheumatoid arthritis. These diseases often associate with inappropriate activation or regulation of the complement system. C1QA mutations or deficiencies can result in a higher susceptibility to SLE and interactions with C1QB and C1QC are also implicated in this disease. Moreover C1QA influences the pathogenesis of rheumatoid arthritis by modulating inflammation responses.
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1 product image
SDS-PAGE - Recombinant Human C1QA protein (ab157982)
ab157982 on a 12.5% SDS-PAGE stained with Coomassie Blue.
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