Recombinant Human C20orf11 protein is a Human Full Length protein, in the 1 to 228 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M S Y A E K P D E I T K D E W M E K L N N L H V Q R A D M N R L I M N Y L V T E G F K E A A E K F R M E S G I E P S V D L E T L D E R I K I R E M I L K G Q I Q E A I A L I N S L H P E L L D T N R Y L Y F H L Q Q Q H L I E L I R Q R E T E A A L E F A Q T Q L A E Q G E E S R E C L T E M E R T L A L L A F D S P E E S P F G D L L H T M Q R Q K V W S E V N Q A V L D Y E N R E S T P K L A K L L K L L L W A Q N E L D Q K K V K Y P K M T D L S K G V I E E P K
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (PubMed:29911972). Acts as a positive regulator of Wnt signaling pathway by promoting beta-catenin (CTNNB1) nuclear accumulation (PubMed:28829046).
Alternative names
C20orf11, TWA1, GID8, Glucose-induced degradation protein 8 homolog, Two hybrid-associated protein 1 with RanBPM, Twa1
Recommended products
Recombinant Human C20orf11 protein is a Human Full Length protein, in the 1 to 228 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9NWU2-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M S Y A E K P D E I T K D E W M E K L N N L H V Q R A D M N R L I M N Y L V T E G F K E A A E K F R M E S G I E P S V D L E T L D E R I K I R E M I L K G Q I Q E A I A L I N S L H P E L L D T N R Y L Y F H L Q Q Q H L I E L I R Q R E T E A A L E F A Q T Q L A E Q G E E S R E C L T E M E R T L A L L A F D S P E E S P F G D L L H T M Q R Q K V W S E V N Q A V L D Y E N R E S T P K L A K L L K L L L W A Q N E L D Q K K V K Y P K M T D L S K G V I E E P K
- Accession
- Q9NWU2
- Protein length
- Full Length
- Predicted molecular weight
- 29.1 kDa
- Amino acids
- 1 to 228
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab201714 was purified using conventional chromatography techniques.
General info
- Function
Core component of the CTLH E3 ubiquitin-protein ligase complex that selectively accepts ubiquitin from UBE2H and mediates ubiquitination and subsequent proteasomal degradation of the transcription factor HBP1 (PubMed:29911972). Acts as a positive regulator of Wnt signaling pathway by promoting beta-catenin (CTNNB1) nuclear accumulation (PubMed:28829046).
- Sequence similarities
Belongs to the GID8 family.
- Post-translational modifications
Polyubiquitinated through 'Lys-48'-polyubiquitin chains, leading to proteasomal degradation in the absence of Wnt stimulation.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
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In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human C20orf11 protein (ab201714)
15% SDS-PAGE analysis of ab201714 (3μg).
Downloads
Product protocols
- Visit the general protocols
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