Recombinant Human C20orf77 protein is a Human Full Length protein, in the 1 to 326 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Images
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M G S M S S F S E S A L E K K L S E L S N S Q Q S V Q T L S L W L I H H R K H A G P I V S V W H R E L R K A K S N R K L T F L Y L A N D V I Q N S K R K G P E F T R E F E S V L V D A F S H V A R E A D E G C K K P L E R L L N I W Q E R S V Y G G E F I Q Q L K L S M E D S K S P P P K A T E E K K S L K R T F Q Q I Q E E E D D D Y P G S Y S P Q D P S A G P L L T E E L I K A L Q D L E N A A S G D A T V R Q K I A S L P Q E V Q D V S L L E K I T D K E A A E R L S K T V D E A C L L L A E Y N G R L A A E L E D R R Q L A R M L V E Y T Q N Q K D V L S E K E K K L E E Y K Q K L A R V T Q V R K E L K S H I Q S L P D L S L L P N V T G G L A P L P S A G D L F S T D
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation.
Alternative names
C20orf77, CREPT, RPRD1B, Regulation of nuclear pre-mRNA domain-containing protein 1B, Cell cycle-related and expression-elevated protein in tumor
Recommended products
Recombinant Human C20orf77 protein is a Human Full Length protein, in the 1 to 326 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q9NQG5-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.4
Constituents: 79% PBS, 20% Glycerol (glycerin, glycerine), 0.02% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M G S M S S F S E S A L E K K L S E L S N S Q Q S V Q T L S L W L I H H R K H A G P I V S V W H R E L R K A K S N R K L T F L Y L A N D V I Q N S K R K G P E F T R E F E S V L V D A F S H V A R E A D E G C K K P L E R L L N I W Q E R S V Y G G E F I Q Q L K L S M E D S K S P P P K A T E E K K S L K R T F Q Q I Q E E E D D D Y P G S Y S P Q D P S A G P L L T E E L I K A L Q D L E N A A S G D A T V R Q K I A S L P Q E V Q D V S L L E K I T D K E A A E R L S K T V D E A C L L L A E Y N G R L A A E L E D R R Q L A R M L V E Y T Q N Q K D V L S E K E K K L E E Y K Q K L A R V T Q V R K E L K S H I Q S L P D L S L L P N V T G G L A P L P S A G D L F S T D
- Accession
- Q9NQG5
- Protein length
- Full Length
- Predicted molecular weight
- 39.3 kDa
- Amino acids
- 1 to 326
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab202606 was purified using conventional chromatography techniques..
General info
- Function
Interacts with phosphorylated C-terminal heptapeptide repeat domain (CTD) of the largest RNA polymerase II subunit POLR2A, and participates in dephosphorylation of the CTD by RPAP2. Transcriptional regulator which enhances expression of CCND1. Promotes binding of RNA polymerase II to the CCDN1 promoter and to the termination region before the poly-A site but decreases its binding after the poly-A site. Prevents RNA polymerase II from reading through the 3' end termination site and may allow it to be recruited back to the promoter through promotion of the formation of a chromatin loop. Also enhances the transcription of a number of other cell cycle-related genes including CDK2, CDK4, CDK6 and cyclin-E but not CDKN1A, CDKN1B or cyclin-A. Promotes cell proliferation.
- Sequence similarities
Belongs to the UPF0400 (RTT103) family.
- Subcellular localisation
- Nucleus
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Product promise
We are dedicated to supporting your work with high quality reagents and we are here for you every step of the way should you need us.
In the unlikely event of one of our products not working as expected, you are covered by our product promise.
Full details and terms and conditions can be found here:
Terms & Conditions.
1 product image
SDS-PAGE - Recombinant Human C20orf77 protein (ab202606)
15% SDS-PAGE analysis of ab202606 (3 μg).
Downloads
Product protocols
- Visit the general protocols
- Visit troubleshooting
Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.
For licensing inquiries, please contact partnerships@abcam.com