Recombinant Human C4d protein is a Human Full Length protein, in the 957 to 1336 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
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Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His-T7 tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
Amino acid sequence
M A S M T G G Q Q M G R G H H H H H H G N L Y F Q G G E F T L E I P G N S D P N M I P D G D F N S Y V R V T A S D P L D T L G S E G A L S P G G V A S L L R L P R G C G E Q T M I Y L A P T L A A S R Y L D K T E Q W S T L P P E T K D H A V D L I Q K G Y M R I Q Q F R K A D G S Y A A W L S R D S S T W L T A F V L K V L S L A Q E Q V G G S P E K L Q E T S N W L L S Q Q Q A D G S F Q D P C P V L D R S M Q G G L V G N D E T V A L T A F V T I A L H H G L A V F Q D E G A E P L K Q R V E A S I S K A N S F L G E K A S A G L L G A H A A A I T A Y A L T L T K A P V D L L G V A H N N L M A M A Q E T G D N L Y W G S V T G S Q S N A V S P T P A P R N P S D P M P Q A P A L W I E T T A Y A L L H L L L H E G K A E M A D Q A S A W L T R Q G S F Q G G F R S T Q D T V I A L D A L S A Y W I A S H T T E E R G L N V T L S S T G R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens. Derived from proteolytic degradation of complement C4, C4a anaphylatoxin is a mediator of local inflammatory process. It induces the contraction of smooth muscle, increases vascular permeability and causes histamine release from mast cells and basophilic leukocytes.
Alternative names
CO4, CPAMD3, C4B_2, Complement C4-B, Basic complement C4, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 3
Recommended products
Recombinant Human C4d protein is a Human Full Length protein, in the 957 to 1336 aa range, expressed in Escherichia coli, with >90% purity and suitable for SDS-PAGE.
Key facts
- Purity
- >90% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His-T7 tag N-Terminus
- Applications
- SDS-PAGE
- Biologically active
- No
- Accession
- P0C0L5-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 0.32% Tris HCl
Sequence info
Amino acid sequence
- M A S M T G G Q Q M G R G H H H H H H G N L Y F Q G G E F T L E I P G N S D P N M I P D G D F N S Y V R V T A S D P L D T L G S E G A L S P G G V A S L L R L P R G C G E Q T M I Y L A P T L A A S R Y L D K T E Q W S T L P P E T K D H A V D L I Q K G Y M R I Q Q F R K A D G S Y A A W L S R D S S T W L T A F V L K V L S L A Q E Q V G G S P E K L Q E T S N W L L S Q Q Q A D G S F Q D P C P V L D R S M Q G G L V G N D E T V A L T A F V T I A L H H G L A V F Q D E G A E P L K Q R V E A S I S K A N S F L G E K A S A G L L G A H A A A I T A Y A L T L T K A P V D L L G V A H N N L M A M A Q E T G D N L Y W G S V T G S Q S N A V S P T P A P R N P S D P M P Q A P A L W I E T T A Y A L L H L L L H E G K A E M A D Q A S A W L T R Q G S F Q G G F R S T Q D T V I A L D A L S A Y W I A S H T T E E R G L N V T L S S T G R
- Accession
- P0C0L4
- Protein length
- Full Length
- Predicted molecular weight
- 43.9 kDa
- Amino acids
- 957 to 1336
- Nature
- Recombinant
- Tags
- His-T7 tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab198640 was expressed in E.coli as inclusion bodies, refolded using unique “temperature shift inclusion body refolding” technology, chromatographically purified and sterile filtered.
General info
- Function
Non-enzymatic component of the C3 and C5 convertases and thus essential for the propagation of the classical complement pathway. Covalently binds to immunoglobulins and immune complexes and enhances the solubilization of immune aggregates and the clearance of IC through CR1 on erythrocytes. C4A isotype is responsible for effective binding to form amide bonds with immune aggregates or protein antigens, while C4B isotype catalyzes the transacylation of the thioester carbonyl group to form ester bonds with carbohydrate antigens.
- Post-translational modifications
Prior to secretion, the single-chain precursor is enzymatically cleaved to yield non-identical chains alpha, beta and gamma. During activation, the alpha chain is cleaved by C1 into C4a and C4b, and C4b stays linked to the beta and gamma chains. Further degradation of C4b by C1 into the inactive fragments C4c and C4d blocks the generation of C3 convertase. The proteolytic cleavages often are incomplete so that many structural forms can be found in plasma.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage conditions
- -80°C
- Appropriate long-term storage conditions
- -80°C
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C4d also known as the C4d complement is a degradation product of the complement component C4. It has a molecular weight of approximately 45 kDa. This protein is commonly expressed on the surfaces of endothelial cells in various tissues where it plays a role in the immune response. The presence of C4d on tissues is often detected using techniques like immunohistochemistry (IHC) also referred to as C4d IHC or C4d immunostain providing valuable insights into complement activation.
- Biological function summary
The C4d protein acts as a marker for complement activation and is part of the larger complement cascade. C4d is primarily associated with the classical pathway and serves as an indicator of complement-mediated cell injury. The degradation of C4 into C4d is usually seen in immune responses where antibodies recognize antigens triggering sequence activation in the complement pathway. C4d's role as a marker facilitates the differentiation between antibody-mediated injury and other types of tissue damage.
- Pathways
This complement protein gets involved in the classical pathway of the complement system which plays a critical role in innate and adaptive immunity. It is related to other proteins like C3 and C1q which are additional components within this pathway. These interactions are significant for the clearance of pathogens and also assist in enhancing the humoral immune response by promoting the deposition of complement on pathogenic surfaces.
- Associated diseases and disorders
C4d presence becomes particularly significant in conditions like transplant rejection and systemic lupus erythematosus (SLE). In transplant rejection its deposition on tissue samples might indicate antibody-mediated rejection where C4d binds to the tissue signaling an immune response against the transplanted organ. Additionally in SLE C4d's relation to other proteins such as C3 and C1q confirms its involvement in autoimmune responses where the immune system mistakenly targets the body's own tissues leading to chronic inflammation and damage.
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SDS-PAGE - Recombinant Human C4d protein (ab198640)
SDS PAGE analysis of ab198640, using BSA as a reference control protein. 4-20% gradient gel was used.
Lane 1: 10μg BSA
Lane 2: 5μg BSA
Lane 3: 1μg BSA
Lane 4: 10μl ab198640
Lane 5: 5μl ab198640
Lane 6: 1μl ab198640
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