Recombinant human C5a protein is a Human Fragment protein, in the 679 to 751 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
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Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
Amino acid sequence
L Q K K I E E I A A K Y K H S V V K K C C Y D G A C V N N D E T C E Q R A A R I S L G P R C I K A F T E C C V V A S Q L R A N I S H K D M Q L G R
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application FuncS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled. C5a anaphylatoxin. Derived from proteolytic degradation of complement C5, C5a anaphylatoxin is a mediator of local inflammatory process. Binding to the receptor C5AR1 induces a variety of responses including intracellular calcium release, contraction of smooth muscle, increased vascular permeability, and histamine release from mast cells and basophilic leukocytes (PubMed:8182049). C5a is also a potent chemokine which stimulates the locomotion of polymorphonuclear leukocytes and directs their migration toward sites of inflammation.
Alternative names
CPAMD4, C5, Complement C5, C3 and PZP-like alpha-2-macroglobulin domain-containing protein 4
Recommended products
Recombinant human C5a protein is a Human Fragment protein, in the 679 to 751 aa range, expressed in Escherichia coli, with >95% purity, < 1 EU/µg endotoxin level and suitable for SDS-PAGE, FuncS.
Key facts
- Purity
- >95% SDS-PAGE
- Endotoxin level
- < 1 EU/µg
- Expression system
- Escherichia coli
- Tags
- Tag free
- Applications
- SDS-PAGE, FuncS
- Biologically active
- Yes
- Biological activity
- ab167724 is active based on enzyme release assay of myeloperoxidase. The ED50 = 1.36 nM.
- Accession
- P01031-1
- Animal free
- No
- Carrier free
- Yes
- Species
- Human
- Reconstitution
- Reconstitute with sterile deionized water to a concentration of 400 µg/ml.
- Concentration
- Storage buffer
pH: 7.4
Constituents: 99% PBS
Sequence info
Amino acid sequence
- L Q K K I E E I A A K Y K H S V V K K C C Y D G A C V N N D E T C E Q R A A R I S L G P R C I K A F T E C C V V A S Q L R A N I S H K D M Q L G R
- Accession
- P01031
- Protein length
- Fragment
- Predicted molecular weight
- 8.3 kDa
- Amino acids
- 679 to 751
- Nature
- Recombinant
Specifications
- Form
- Lyophilized
General info
- Function
Activation of C5 by a C5 convertase initiates the spontaneous assembly of the late complement components, C5-C9, into the membrane attack complex. C5b has a transient binding site for C6. The C5b-C6 complex is the foundation upon which the lytic complex is assembled.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
This product is an active protein and may elicit a biological response in vivo, handle with caution.
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
C5a also known as complement component 5a is a 74-amino acid peptide with a molecular mass of approximately 11 kDa. It is expressed in the blood and is a fragment that forms when the complement system part of the body’s immune response activates. As a potent anaphylatoxin C5a plays a role in mediating inflammation and recruiting white blood cells to an infection site. It interacts with C5a receptors C5aR1 and C5aR2 located on various immune cells including neutrophils monocytes and macrophages.
- Biological function summary
C5a functions as part of the complement cascade a complex series of events essential for opsonization cell lysis and inflammation. C5a binds to its receptors initiating cell responses such as chemotaxis increased vascular permeability and release of pro-inflammatory cytokines. These processes help in pathogen clearance making C5a a significant player in innate immunity. C5a exists as part of the larger complement system that includes proteins like C3 and C5 forming an important arm of the body's defense against pathogens.
- Pathways
C5a integrates into the complement pathway and the coagulation cascade. It drives actions in the inflammatory response enhancing immune defense by promoting cytokine production and leukocyte migration. Important interactions occur with complement proteins such as C3 and C5b-9 key elements of the terminal complement complex. By influencing these pathways C5a directly contributes to both immune modulation and inflammatory processes.
- Associated diseases and disorders
C5a is linked to conditions such as sepsis and rheumatoid arthritis. In these diseases C5a contributes to excessive inflammation and tissue damage. C5a's interaction with C5aR1 and other receptors like CR1 involved in inflammatory regulation exemplifies its role in advancing disease states. Therapeutically targeting C5a and its receptors remains an active area of research aiming to develop treatments for conditions driven by dysregulated complement activation.
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4 product images
SDS-PAGE - Recombinant human C5a protein (ab167724)
Human C5a, Tag Free on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Functional Studies - Recombinant human C5a protein (ab167724)
Immobilized Human C5a, Tag Free at 2 µg/mL (100 µL/well) can bind Anti-C5a Mab, Human IgG1 with a linear range of 0.8-13 ng/mL.
Functional Studies - Recombinant human C5a protein (ab167724)
Human C5a, Tag Free induce N-acetyl-?-D-glucosaminidase release from differentiated U937 cells. The ED50 for this effect is 0.215-0.323 µg/mL.
SDS-PAGE - Recombinant human C5a protein (ab167724)
SDS-PAGE of reduced ab167724 stained overnight with Coomassie Blue (6μg).
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