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AB135006

Recombinant Human C6orf134/TAT protein

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Recombinant Human C6orf134/TAT protein is a Human Fragment protein, in the 2 to 224 aa range, expressed in Escherichia coli, with >40%, suitable for SDS-PAGE, FuncS.

View Alternative Names

C6orf134, MEC17, Nbla00487, ATAT1, Alpha-tubulin N-acetyltransferase 1, Alpha-TAT, Alpha-TAT1, TAT, Acetyltransferase mec-17 homolog

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SDS-PAGE - Recombinant Human C6orf134/TAT protein (AB135006)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human C6orf134/TAT protein (AB135006)

10% SDS-PAGE analysis of ab135006 at 3.2 μg stained with Coomassie.

Key facts

Purity

>40% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

FuncS, SDS-PAGE

applications

Biologically active

No

Accession

Q5SQI0

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 20% Glycerol (glycerin, glycerine), 0.65% Sodium chloride, 0.64% Tris HCl, 0.5% Glutathione, 0.05% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.017% Potassium chloride

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "FuncS": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as C6orf134
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Sequence info

[{"sequence":"MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKKFELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRYGVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVLYMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPKSDLEVLFQGPLGSWLTWPFCFLTITLREEGVCHLESVDLQQQIMTIIDELGKASAKAQNLSAPITSASRMQSNRHVVYILKDSSARPAGKGAIIGFIKVGYKKLFVLDDREAHNEVEPLCILDFYIHESVQRHGHGRELFQYMLQKERVEPHQLAIDRPSQKLLKFLNKHYNLETTVPQVNNFVIFEGFFAHQHRPPAPSLRATRHSRAAAVDPTPAAPARKLPPKRAEGDIKPYS","proteinLength":"Fragment","predictedMolecularWeight":"52 kDa","actualMolecularWeight":null,"aminoAcidEnd":224,"aminoAcidStart":2,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"Q5SQI0","tags":[]}]
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Properties and storage information

Shipped at conditions
Dry Ice
Appropriate short-term storage conditions
-80°C
Appropriate long-term storage conditions
-80°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

C6orf134 also known as TAT codes for a protein that functions in enzymatic activity catalyzing chemical reactions within cells. This protein has a molecular mass of approximately 59 kDa. It is found expressed in a variety of tissues but shows higher expression in liver tissue. Its enzymatic role involves processes that rely on its catalytic ability impacting cell metabolism and energy regulation.
Biological function summary

The enzyme affects metabolic pathways and amino acid metabolism. It plays a part in the conversion of specific substrates within these systems balancing energy flow and the availability of key metabolites. It can function as either an isolated enzyme or as part of larger enzymatic complexes depending on the cellular environment and metabolic demands.

Pathways

C6orf134 is deeply involved in the urea cycle and gluconeogenesis. These pathways play a critical role in managing nitrogen disposal and producing glucose when energy is needed. The enzyme operates alongside proteins such as carbamoyl phosphate synthetase 1 in the urea cycle and is related to phosphoenolpyruvate carboxykinase in gluconeogenesis affecting both nitrogen balance and glucose production.

Deficiencies or dysregulations of the enzyme relate to metabolic disorders such as tyrosinemia and hyperammonemia. These conditions connect C6orf134 to proteins like fumarylacetoacetate hydrolase and ornithine transcarbamylase as they are part of the metabolic networks disrupted in these disorders. Studying these connections helps understand how metabolic imbalances lead to disease symptoms.
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Specifications

Form

Liquid

Additional notes

Lot specific.

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General info

Function

Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.

Sequence similarities

Belongs to the acetyltransferase ATAT1 family.

Post-translational modifications

Autoacetylation strongly increases tubulin acetylation.

Subcellular localisation

Cytoskeleton

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Product protocols

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Target data

Specifically acetylates 'Lys-40' in alpha-tubulin on the lumenal side of microtubules. Promotes microtubule destabilization and accelerates microtubule dynamics; this activity may be independent of acetylation activity. Acetylates alpha-tubulin with a slow enzymatic rate, due to a catalytic site that is not optimized for acetyl transfer. Enters the microtubule through each end and diffuses quickly throughout the lumen of microtubules. Acetylates only long/old microtubules because of its slow acetylation rate since it does not have time to act on dynamically unstable microtubules before the enzyme is released. Required for normal sperm flagellar function. Promotes directional cell locomotion and chemotaxis, through AP2A2-dependent acetylation of alpha-tubulin at clathrin-coated pits that are concentrated at the leading edge of migrating cells. May facilitate primary cilium assembly.
See full target information ATAT1
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