Recombinant Human Calcineurin A protein is a Human Full Length protein, in the 1 to 511 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, WB.
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Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H T G S M S E P K A I D P K L S T T D R V V K A V P F P P S H R L T A K E V F D N D G K P R V D I L K A H L M K E G R L E E S V A L R I I T E G A S I L R Q E K N L L D I D A P V T V C G D I H G Q F F D L M K L F E V G G S P A N T R Y L F L G D Y V D R G Y F S I E C V L Y L W A L K I L Y P K T L F L L R G N H E C R H L T E Y F T F K Q E C K I K Y S E R V Y D A C M D A F D C L P L A A L M N Q Q F L C V H G G L S P E I N T L D D I R K L D R F K E P P A Y G P M C D I L W S D P L E D F G N E K T Q E H F T H N T V R G C S Y F Y S Y P A V C E F L Q H N N L L S I L R A H E A Q D A G Y R M Y R K S Q T T G F P S L I T I F S A P N Y L D V Y N N K A A V L K Y E N N V M N I R Q F N C S P H P Y W L P N F M D V F T W S L P F V G E K V T E M L V N V L N I C S D D E L G S E E D G F D G A T A A A R K E V I R N K I R A I G K M A R V F S V L R E E S E S V L T L K G L T P T G M L P S G V L S G G K Q T L Q S A I K G F S P Q H K I T S F E E A K G L D R I N E R M P P R R D A M P S D A N L N S I N K A L T S E T N G T D S N G S N S S N I Q
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application WB | Reactivity Reacts | Dilution info - | Notes ab101870 can be used as a WB positive control in conjunction with Anti-Calcineurin A antibody ab3673. |
Associated Products
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Target data
Function
Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:15671020, PubMed:18838687, PubMed:19154138, PubMed:23468591, PubMed:30254215). Many of the substrates contain a PxIxIT motif and/or a LxVP motif (PubMed:17498738, PubMed:17502104, PubMed:22343722, PubMed:23468591, PubMed:27974827). In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation (PubMed:15671020). In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion (PubMed:18838687). Positively regulates the CACNA1B/CAV2.2-mediated Ca(2+) release probability at hippocampal neuronal soma and synaptic terminals (By similarity). Dephosphorylates heat shock protein HSPB1 (By similarity). Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138). In response to increased Ca(2+) levels, regulates NFAT-mediated transcription probably by dephosphorylating NFAT and promoting its nuclear translocation (PubMed:26248042). Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138). Dephosphorylates DARPP32 (PubMed:19154138). May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins (PubMed:30611118). Dephosphorylates transcription factor TFEB at 'Ser-211' following Coxsackievirus B3 infection, promoting nuclear translocation (PubMed:33691586). Required for postnatal development of the nephrogenic zone and superficial glomeruli in the kidneys, cell cycle homeostasis in the nephrogenic zone, and ultimately normal kidney function (By similarity). Plays a role in intracellular AQP2 processing and localization to the apical membrane in the kidney, may thereby be required for efficient kidney filtration (By similarity). Required for secretion of salivary enzymes amylase, peroxidase, lysozyme and sialic acid via formation of secretory vesicles in the submandibular glands (By similarity). Required for calcineurin activity and homosynaptic depotentiation in the hippocampus (By similarity). Required for normal differentiation and survival of keratinocytes and therefore required for epidermis superstructure formation (By similarity). Positively regulates osteoblastic bone formation, via promotion of osteoblast differentiation (By similarity). Positively regulates osteoclast differentiation, potentially via NFATC1 signaling (By similarity). May play a role in skeletal muscle fiber type specification, potentially via NFATC1 signaling (By similarity). Negatively regulates MAP3K14/NIK signaling via inhibition of nuclear translocation of the transcription factors RELA and RELB (By similarity). Required for antigen-specific T-cell proliferation response (By similarity). Dephosphorylates KLHL3, promoting the interaction between KLHL3 and WNK4 and subsequent degradation of WNK4 (PubMed:30718414). Negatively regulates SLC9A1 activity (PubMed:31375679).
Alternative names
CALNA, CNA, PPP3CA, Protein phosphatase 3 catalytic subunit alpha, CAM-PRP catalytic subunit, Calcineurin A alpha, Calmodulin-dependent calcineurin A subunit alpha isoform, Serine/threonine-protein phosphatase 2B catalytic subunit alpha isoform, CNA alpha
Recommended products
Recombinant Human Calcineurin A protein is a Human Full Length protein, in the 1 to 511 aa range, expressed in Escherichia coli, with >85% purity and suitable for SDS-PAGE, WB.
Key facts
- Purity
- >85% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, WB
- Biologically active
- No
- Accession
- Q08209-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 20% Glycerol (glycerin, glycerine), 1.16% Sodium chloride, 0.316% Tris HCl, 0.077% (R*,R*)-1,4-Dimercaptobutan-2,3-diol, 0.0292% EDTA
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H T G S M S E P K A I D P K L S T T D R V V K A V P F P P S H R L T A K E V F D N D G K P R V D I L K A H L M K E G R L E E S V A L R I I T E G A S I L R Q E K N L L D I D A P V T V C G D I H G Q F F D L M K L F E V G G S P A N T R Y L F L G D Y V D R G Y F S I E C V L Y L W A L K I L Y P K T L F L L R G N H E C R H L T E Y F T F K Q E C K I K Y S E R V Y D A C M D A F D C L P L A A L M N Q Q F L C V H G G L S P E I N T L D D I R K L D R F K E P P A Y G P M C D I L W S D P L E D F G N E K T Q E H F T H N T V R G C S Y F Y S Y P A V C E F L Q H N N L L S I L R A H E A Q D A G Y R M Y R K S Q T T G F P S L I T I F S A P N Y L D V Y N N K A A V L K Y E N N V M N I R Q F N C S P H P Y W L P N F M D V F T W S L P F V G E K V T E M L V N V L N I C S D D E L G S E E D G F D G A T A A A R K E V I R N K I R A I G K M A R V F S V L R E E S E S V L T L K G L T P T G M L P S G V L S G G K Q T L Q S A I K G F S P Q H K I T S F E E A K G L D R I N E R M P P R R D A M P S D A N L N S I N K A L T S E T N G T D S N G S N S S N I Q
- Accession
- Q08209
- Protein length
- Full Length
- Predicted molecular weight
- 60 kDa
- Amino acids
- 1 to 511
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab101870 was purified using conventional chromatography techniques.
General info
- Function
Calcium-dependent, calmodulin-stimulated protein phosphatase which plays an essential role in the transduction of intracellular Ca(2+)-mediated signals (PubMed:15671020, PubMed:18838687, PubMed:19154138, PubMed:23468591, PubMed:30254215). Many of the substrates contain a PxIxIT motif and/or a LxVP motif (PubMed:17498738, PubMed:17502104, PubMed:22343722, PubMed:23468591, PubMed:27974827). In response to increased Ca(2+) levels, dephosphorylates and activates phosphatase SSH1 which results in cofilin dephosphorylation (PubMed:15671020). In response to increased Ca(2+) levels following mitochondrial depolarization, dephosphorylates DNM1L inducing DNM1L translocation to the mitochondrion (PubMed:18838687). Positively regulates the CACNA1B/CAV2.2-mediated Ca(2+) release probability at hippocampal neuronal soma and synaptic terminals (By similarity). Dephosphorylates heat shock protein HSPB1 (By similarity). Dephosphorylates and activates transcription factor NFATC1 (PubMed:19154138). In response to increased Ca(2+) levels, regulates NFAT-mediated transcription probably by dephosphorylating NFAT and promoting its nuclear translocation (PubMed:26248042). Dephosphorylates and inactivates transcription factor ELK1 (PubMed:19154138). Dephosphorylates DARPP32 (PubMed:19154138). May dephosphorylate CRTC2 at 'Ser-171' resulting in CRTC2 dissociation from 14-3-3 proteins (PubMed:30611118). Dephosphorylates transcription factor TFEB at 'Ser-211' following Coxsackievirus B3 infection, promoting nuclear translocation (PubMed:33691586). Required for postnatal development of the nephrogenic zone and superficial glomeruli in the kidneys, cell cycle homeostasis in the nephrogenic zone, and ultimately normal kidney function (By similarity). Plays a role in intracellular AQP2 processing and localization to the apical membrane in the kidney, may thereby be required for efficient kidney filtration (By similarity). Required for secretion of salivary enzymes amylase, peroxidase, lysozyme and sialic acid via formation of secretory vesicles in the submandibular glands (By similarity). Required for calcineurin activity and homosynaptic depotentiation in the hippocampus (By similarity). Required for normal differentiation and survival of keratinocytes and therefore required for epidermis superstructure formation (By similarity). Positively regulates osteoblastic bone formation, via promotion of osteoblast differentiation (By similarity). Positively regulates osteoclast differentiation, potentially via NFATC1 signaling (By similarity). May play a role in skeletal muscle fiber type specification, potentially via NFATC1 signaling (By similarity). Negatively regulates MAP3K14/NIK signaling via inhibition of nuclear translocation of the transcription factors RELA and RELB (By similarity). Required for antigen-specific T-cell proliferation response (By similarity). Dephosphorylates KLHL3, promoting the interaction between KLHL3 and WNK4 and subsequent degradation of WNK4 (PubMed:30718414). Negatively regulates SLC9A1 activity (PubMed:31375679).
- Sequence similarities
Belongs to the PPP phosphatase family. PP-2B subfamily.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calcineurin A also known as PPP3CA is a highly specific protein phosphatase with a pivotal role in cellular signaling. It has a molecular mass of approximately 59 to 61 kDa depending on the isoform. Calcineurin A expression occurs in various tissues including the brain immune cells and cardiac tissues. It acts as the catalytic subunit of the enzyme calcineurin and is important for its function.
- Biological function summary
Calcineurin A participates in numerous cellular processes through its phosphatase activity. As part of the calcineurin complex which includes a regulatory subunit it modulates the dephosphorylation of target proteins. This activity enables Calcineurin A to influence activation of T-lymphocytes by dephosphorylating the nuclear factor of activated T-cells (NFAT) a transcription factor responsible for immune response. Calcineurin A also plays a role in neuronal signaling and muscle function.
- Pathways
Calcineurin A engages in the calcium signaling pathway which is vital for diverse cellular functions. It is related to proteins like calmodulin which binds calcium ions to activate Calcineurin A. Another important pathway includes the MAPK signaling pathway where Calcineurin A influences various cellular outcomes by regulating different transcription factors. Through these pathways Calcineurin A integrates signals that affect cellular growth survival and differentiation.
- Associated diseases and disorders
Calcineurin A is associated with cardiac hypertrophy and immunosuppressive conditions. In cardiac hypertrophy aberrant Calcineurin A activity leads to changes in heart muscle size and function. The protein also connects to the disorder of immunosuppression through its inhibition by drugs like cyclosporine which are used to prevent transplant rejection by targeting Calcineurin A. This inhibition blocks T-cell activation and provides therapeutic benefits in managing transplant patients.
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3 product images
SDS-PAGE - Recombinant Human Calcineurin A protein (ab101870)
15% SDS PAGE analysis of 3μg ab101870.
Western blot - Recombinant Human Calcineurin A protein (ab101870)
All lanes: Western blot - Anti-Calcineurin A antibody (Anti-Calcineurin A antibody ab3673) at 1/1000 dilution
All lanes: Western blot - Recombinant Human Calcineurin A protein (ab101870) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Exposure time: 10s
Western blot - Recombinant Human Calcineurin A protein (ab101870)
All lanes: Western blot - Anti-Calcineurin A antibody (Anti-Calcineurin A antibody ab3673) at 1/1000 dilution
All lanes: Western blot - Recombinant Human Calcineurin A protein (ab101870) at 0.1 µg
SecondaryAll lanes: Western blot - Goat Anti-Rabbit IgG H&L (HRP) preadsorbed (Goat Anti-Rabbit IgG H&L (HRP) preadsorbed ab97080) at 1/5000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 59 kDa
Exposure time: 10s
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