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AB103786

Recombinant Human Calcineurin B/PPP3R1 protein

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(1 Publication)

Recombinant Human Calcineurin B/PPP3R1 protein is a Human Full Length protein, in the 1 to 170 aa range, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE, Mass Spec.

View Alternative Names

CNA2, CNB, PPP3R1, Calcineurin subunit B type 1, Protein phosphatase 2B regulatory subunit 1, Protein phosphatase 3 regulatory subunit B alpha isoform 1

1 Images
SDS-PAGE - Recombinant Human Calcineurin B/PPP3R1 protein (AB103786)
  • SDS-PAGE

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SDS-PAGE - Recombinant Human Calcineurin B/PPP3R1 protein (AB103786)

15% SDS-PAGE analysis of 3μg ab103786.

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

His tag N-Terminus

Applications

SDS-PAGE, Mass Spec

applications

Biologically active

No

Accession

P63098

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 8 Constituents: 10% Glycerol (glycerin, glycerine), 0.58% Sodium chloride, 0.316% Tris HCl, 0.0308% (R*,R*)-1,4-Dimercaptobutan-2,3-diol

storage-buffer

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Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" }, "Mass Spec": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
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Product details

This product was previously labelled as Calcineurin B and Calcineurin B/CNB.
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Sequence info

[{"sequence":"MGSSHHHHHHSSGLVPRGSHMGNEASYPLEMCSHFDADEIKRLGKRFKKLDLDNSGSLSVEEFMSLPELQQNPLVQRVIDIFDTDGNGEVDFKEFIEGVSQFSVKGDKEQKLRFAFRIYDMDKDGYISNGELFQVLKMMVGNNLKDTQLQQIVDKTIINADKDGDGRISFEEFCAVVGGLDIHKKMVVDV","proteinLength":"Full Length","predictedMolecularWeight":"21.5 kDa","actualMolecularWeight":null,"aminoAcidEnd":170,"aminoAcidStart":1,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":"P63098","tags":[{"tag":"His","terminus":"N-Terminus"}]}]
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Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
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Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calcineurin B also known as PPP3R1 serves as the regulatory subunit of the calcineurin phosphatase complex. The protein possesses a molecular mass of roughly 19 kDa. It expresses widely in various tissues especially in the brain skeletal muscle and immune cells. Calcineurin B partners with the catalytic subunit Calcineurin A to form the complete enzyme necessary for its dephosphorylation activity.
Biological function summary

Calcineurin B plays an important role in regulating calcium signaling pathways. It functions as part of a heterodimeric complex where it binds calcium ions and is necessary for activating the serine/threonine phosphatase activity of calcineurin. Through this activity calcineurin B influences the transcriptional activation of NFAT (Nuclear Factor of Activated T-cells) and other downstream signaling pathways affecting cell growth differentiation and immune responses.

Pathways

Calcineurin B acts critically in the calcium/calmodulin-dependent signaling pathway and the T-cell receptor signaling pathway. In these pathways it regulates the activity of proteins like NFAT and influences downstream effects essential for immune responses. The dephosphorylation activity facilitated by calcineurin B also connects it with a network of signaling cascades modulating calcium levels impacting cellular activities significantly.

Calcineurin B is associated with immunosuppressive outcomes and neurodegenerative conditions. Its connection with NFAT makes it a target for immune-related disorders such as autoimmune diseases and transplant rejection. Additionally alterations in its activity connect with proteins like tau contributing to disorders like Alzheimer's disease due to dysregulated calcium signaling and neuronal damage.
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Specifications

Form

Liquid

Additional notes

ab103786 was purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

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General info

Function

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.

Sequence similarities

Belongs to the calcineurin regulatory subunit family.

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Product protocols

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Target data

Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
See full target information PPP3R1
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Publications (1)

Recent publications for all applications. Explore the full list and refine your search

PloS one 18:e0283943 PubMed37200357

2023

Development of a cell-free screening assay for the identification of direct PERK activators.

Applications

Unspecified application

Species

Unspecified reactive species

Márcia F D Costa,Günter U Höglinger,Thomas W Rösler
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