Recombinant Human Calcineurin BII/PPP3R2 protein is a Human Full Length protein, in the 1 to 173 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M S T M G N E A S Y P A E M C S H F D N D E I K R L G R R F K K L D L D K S G S L S V E E F M S L P E L R H N P L V R R V I D V F D T D G D G E V D F K E F I L G T S Q F S V K G D E E Q K L R F A F S I Y D M D K D G Y I S N G E L F Q V L K M M V G N N L T D W Q L Q Q L V D K T I I I L D K D G D G K I S F E E F S A V V R D L E I H K K L V L I V
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
Alternative names
CBLP, PPP3RL, PPP3R2, Calcineurin subunit B type 2, Calcineurin B-like protein, Calcineurin BII, PPP3R1-like, Protein phosphatase 2B regulatory subunit 2, Protein phosphatase 3 regulatory subunit B beta isoform, CNBII
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Recombinant Human Calcineurin BII/PPP3R2 protein is a Human Full Length protein, in the 1 to 173 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q96LZ3-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 8
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl, 0.0154% (R*,R*)-1,4-Dimercaptobutan-2,3-diol
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M S T M G N E A S Y P A E M C S H F D N D E I K R L G R R F K K L D L D K S G S L S V E E F M S L P E L R H N P L V R R V I D V F D T D G D G E V D F K E F I L G T S Q F S V K G D E E Q K L R F A F S I Y D M D K D G Y I S N G E L F Q V L K M M V G N N L T D W Q L Q Q L V D K T I I I L D K D G D G K I S F E E F S A V V R D L E I H K K L V L I V
- Accession
- Q96LZ3
- Protein length
- Full Length
- Predicted molecular weight
- 22 kDa
- Amino acids
- 1 to 173
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
Purified using conventional chromatography techniques.
General info
- Function
Regulatory subunit of calcineurin, a calcium-dependent, calmodulin stimulated protein phosphatase. Confers calcium sensitivity.
- Sequence similarities
Belongs to the calcineurin regulatory subunit family.
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calcineurin BII also known as PPP3R2 is a regulatory subunit of the calcineurin heterodimeric complex. Calcineurin BII mainly constitutes the regulatory component of this phosphatase which has a molecular mass of approximately 19 kDa. It is expressed in various tissues with significant presence in the brain and immune cells. Calcineurin BII interacts with the catalytic subunit (calcineurin A) to form the active calcineurin enzyme which is an important player in several intracellular processes.
- Biological function summary
Calcineurin BII serves as a calcium sensor that regulates the enzyme's phosphatase activity. The interaction with calcineurin A forms the dimeric complex necessary for its activity. This complex plays an important role in dephosphorylating proteins which is essential for modifying protein function and signaling pathways. Calcineurin BII controls cellular responses like T-cell activation by responding to calcium influxes impacting immune responses and neuronal signaling.
- Pathways
Calcineurin BII has important implications in both the calcium signaling pathway and the immune signaling pathway. In the calcium signaling pathway calcineurin BII works alongside the calcineurin A subunit to regulate the dephosphorylation of nuclear factor of activated T-cells (NFAT) a transcription factor. This regulation extends to immune responses as calcineurin BII is instrumental in T-cell receptor signaling where it influences NFAT to modulate gene expression critical for T-cell function.
- Associated diseases and disorders
Calcineurin BII relates to autoimmune diseases and neurodegenerative disorders. In autoimmune diseases abnormal activation of calcineurin BII and its associated pathways can cause excessive immune responses. Meanwhile in neurodegenerative disorders its dysregulation affects neuronal signaling pathways potentially adding to disease pathogenesis. Calcineurin BII further interacts with proteins like NFAT in these disease contexts emphasizing its role in pathological signaling alterations.
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2 product images
SDS-PAGE - Recombinant Human Calcineurin BII/PPP3R2 protein (ab105609)
15% SDS-PAGE analysis of ab105609 (3 μg).
SDS-PAGE - Recombinant Human Calcineurin BII/PPP3R2 protein (ab105609)
SDS PAGE analysis of ab105609
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