Recombinant Human Calcium binding protein P22 is a Human Full Length protein, in the 1 to 195 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
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Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
Amino acid sequence
M G S S H H H H H H S S G L V P R G S H M M G S R A S T L L R D E E L E E I K K E T G F S H S Q I T R L Y S R F T S L D K G E N G T L S R E D F Q R I P E L A I N P L G D R I I N A F F P E G E D Q V N F R G F M R T L A H F R P I E D N E K S K D V N G P E P L N S R S N K L H F A F R L Y D L D K D E K I S R D E L L Q V L R M M V G V N I S D E Q L G S I A D R T I Q E A D Q D G D S A I S F T E F V K V L E K V D V E Q K M S I R F L H
Reactivity data
| Application | Reactivity | Dilution info | Notes |
|---|---|---|---|
Application SDS-PAGE | Reactivity Reacts | Dilution info - | Notes - |
Application MS | Reactivity Reacts | Dilution info - | Notes - |
Target data
Function
Calcium-binding protein involved in different processes such as regulation of vesicular trafficking, plasma membrane Na(+)/H(+) exchanger and gene transcription. Involved in the constitutive exocytic membrane traffic. Mediates the association between microtubules and membrane-bound organelles of the endoplasmic reticulum and Golgi apparatus and is also required for the targeting and fusion of transcytotic vesicles (TCV) with the plasma membrane. Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Affects the pH sensitivity of SLC9A1/NHE1 by increasing its sensitivity at acidic pH. Required for the stabilization and localization of SLC9A1/NHE1 at the plasma membrane. Inhibits serum- and GTPase-stimulated Na(+)/H(+) exchange. Plays a role as an inhibitor of ribosomal RNA transcription by repressing the nucleolar UBF1 transcriptional activity. May sequester UBF1 in the nucleoplasm and limit its translocation to the nucleolus. Associates to the ribosomal gene promoter. Acts as a negative regulator of the calcineurin/NFAT signaling pathway. Inhibits NFAT nuclear translocation and transcriptional activity by suppressing the calcium-dependent calcineurin phosphatase activity. Also negatively regulates the kinase activity of the apoptosis-induced kinase STK17B. Inhibits both STK17B auto- and substrate-phosphorylations in a calcium-dependent manner.
Alternative names
CHP, CHP1, Calcineurin B homologous protein 1, Calcineurin B-like protein, Calcium-binding protein CHP, Calcium-binding protein p22, EF-hand calcium-binding domain-containing protein p22
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Recombinant Human Calcium binding protein P22 is a Human Full Length protein, in the 1 to 195 aa range, expressed in Escherichia coli, with >95% purity and suitable for SDS-PAGE, MS.
Key facts
- Purity
- >95% SDS-PAGE
- Expression system
- Escherichia coli
- Tags
- His tag N-Terminus
- Applications
- SDS-PAGE, MS
- Biologically active
- No
- Accession
- Q99653-1
- Animal free
- No
- Species
- Human
- Concentration
- Storage buffer
pH: 7.5
Constituents: 10% Glycerol (glycerin, glycerine), 0.316% Tris HCl
Sequence info
Amino acid sequence
- M G S S H H H H H H S S G L V P R G S H M M G S R A S T L L R D E E L E E I K K E T G F S H S Q I T R L Y S R F T S L D K G E N G T L S R E D F Q R I P E L A I N P L G D R I I N A F F P E G E D Q V N F R G F M R T L A H F R P I E D N E K S K D V N G P E P L N S R S N K L H F A F R L Y D L D K D E K I S R D E L L Q V L R M M V G V N I S D E Q L G S I A D R T I Q E A D Q D G D S A I S F T E F V K V L E K V D V E Q K M S I R F L H
- Accession
- Q99653
- Protein length
- Full Length
- Predicted molecular weight
- 24.7 kDa
- Amino acids
- 1 to 195
- Nature
- Recombinant
- Tags
- His tag N-Terminus
Specifications
- Form
- Liquid
- Additional notes
ab99141 was purified by using conventional chromatography.
General info
- Function
Calcium-binding protein involved in different processes such as regulation of vesicular trafficking, plasma membrane Na(+)/H(+) exchanger and gene transcription. Involved in the constitutive exocytic membrane traffic. Mediates the association between microtubules and membrane-bound organelles of the endoplasmic reticulum and Golgi apparatus and is also required for the targeting and fusion of transcytotic vesicles (TCV) with the plasma membrane. Functions as an integral cofactor in cell pH regulation by controlling plasma membrane-type Na(+)/H(+) exchange activity. Affects the pH sensitivity of SLC9A1/NHE1 by increasing its sensitivity at acidic pH. Required for the stabilization and localization of SLC9A1/NHE1 at the plasma membrane. Inhibits serum- and GTPase-stimulated Na(+)/H(+) exchange. Plays a role as an inhibitor of ribosomal RNA transcription by repressing the nucleolar UBF1 transcriptional activity. May sequester UBF1 in the nucleoplasm and limit its translocation to the nucleolus. Associates to the ribosomal gene promoter. Acts as a negative regulator of the calcineurin/NFAT signaling pathway. Inhibits NFAT nuclear translocation and transcriptional activity by suppressing the calcium-dependent calcineurin phosphatase activity. Also negatively regulates the kinase activity of the apoptosis-induced kinase STK17B. Inhibits both STK17B auto- and substrate-phosphorylations in a calcium-dependent manner.
- Sequence similarities
Belongs to the calcineurin regulatory subunit family. CHP subfamily.
- Post-translational modifications
Phosphorylated; decreased phosphorylation is associated with an increase in SLC9A1/NHE1 Na(+)/H(+) exchange activity. Phosphorylation occurs in serum-dependent manner. The phosphorylation state may regulate the binding to SLC9A1/NHE1.
- Subcellular localisation
- Nucleus, Cytoskeleton
Storage
- Shipped at conditions
- Blue Ice
- Appropriate short-term storage duration
- 1-2 weeks
- Appropriate short-term storage conditions
- +4°C
- Appropriate long-term storage conditions
- -20°C
- Aliquoting information
- Upon delivery aliquot
- Storage information
- Avoid freeze / thaw cycle
Supplementary info
- This supplementary information is collated from multiple sources and compiled automatically.
- Activity summary
Calcium binding protein P22 also known as CABP22 plays a role in calcium signaling by interacting with calcium ions. This protein has a molecular mass of around 22 kDa reflecting its name. Expression of CABP22 occurs in various tissues but predominantly in the brain which suggests its importance in neural activities. Scientists study this protein to understand how neurons respond to calcium fluctuations and consequently how they communicate in the nervous system.
- Biological function summary
CABP22 modulates intracellular calcium levels and is not known to be part of a complex. It influences calcium-dependent tasks such as neurotransmitter release gene expression and protein phosphorylation affecting cellular behavior. Its function is critical in fine-tuning the activity of neurons and maintaining proper neuronal signaling and plasticity.
- Pathways
CABP22 functions within the calcium signaling and synaptic transmission pathways. It influences the activities of related proteins such as calmodulin and troponin which are essential in transducing calcium signals to trigger specific cellular responses. This connection ensures that CABP22 can regulate calcium dynamics in the cellular environment affecting synaptic strength and plasticity.
- Associated diseases and disorders
CABP22 may relate to neurodegenerative diseases such as Alzheimer's disease where calcium homeostasis fails. It associates with proteins like beta-amyloid which accumulates in Alzheimer's suggesting a link between disrupted calcium signaling and disease progression. CABP22 also could connect to epilepsy as imbalances in calcium could lead to aberrant neuronal firing linking to proteins such as ion channels involved in neuronal excitability.
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SDS-PAGE - Recombinant Human Calcium binding protein P22 (ab99141)
15% SDS-PAGE analysis of 3μg ab99141.
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