JavaScript is disabled in your browser. Please enable JavaScript to view this website.
AB78694

Recombinant Human Calmodulin 1/2/3 protein (Tag Free)

Be the first to review this product! Submit a review

|

(0 Publication)

Recombinant Human Calmodulin 1/2/3 protein (Tag Free) is a Human Full Length protein, expressed in Escherichia coli, with >90%, suitable for SDS-PAGE.

View Alternative Names

CALM, CAM, CAM1, CALM1, Calmodulin-1

1 Images
SDS-PAGE - Recombinant Human Calmodulin 1/2/3 protein (AB78694)
  • SDS-PAGE

Unknown

SDS-PAGE - Recombinant Human Calmodulin 1/2/3 protein (AB78694)

15% SDS-PAGE showing ab78694 at approximately 18kDa (3μg).

Key facts

Purity

>90% SDS-PAGE

Expression system

Escherichia coli

Tags

Tag free

Applications

SDS-PAGE

applications

Biologically active

No

Accession

P0DP23

Animal free

No

Carrier free

No

Species

Human

Storage buffer

pH: 7.5 Constituents: 0.242% Tris

storage-buffer

style
left-column

Reactivity data

{ "title": "Reactivity Data", "filters": { "stats": ["", "Reactivity", "Dilution Info", "Notes"] }, "values": { "SDS-PAGE": { "reactivity":"TESTED_AND_REACTS", "dilution-info":"", "notes":"<p></p>" } } }
style
left-column
style
left-column

Sequence info

[{"sequence":"MADQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDADGNGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYISAAELRHVMTNLGEKLTDEEVDEMIREADIDGDGQVNYEEFVQMMTAK","proteinLength":"Full Length","predictedMolecularWeight":null,"actualMolecularWeight":null,"aminoAcidEnd":0,"aminoAcidStart":0,"nature":"Recombinant","expressionSystem":"Escherichia coli","accessionNumber":null,"tags":[]}]
style
left-column

Properties and storage information

Shipped at conditions
Blue Ice
Appropriate short-term storage conditions
-20°C
Appropriate long-term storage conditions
-20°C
Aliquoting information
Upon delivery aliquot
Storage information
Avoid freeze / thaw cycle
False
style
left-column

Supplementary information

This supplementary information is collated from multiple sources and compiled automatically.

Calmodulin also known as calmoduline is a multifunctional intermediate calcium-binding protein expressed widely in eukaryotic cells. This small protein has a molecular weight of approximately 16.7 kDa. Calmodulin 1 2 and 3 serve as the key isoforms facilitating a variety of calcium-mediated processes. You can find it in cellular contexts where calcium signaling plays a regulatory role. Due to its ubiquity it acts as a mediator in numerous cellular responses.
Biological function summary

Calmodulin modulates intracellular activities by binding to calcium ions and interacting with a wide array of target proteins. It is not part of a molecular complex but can partner with proteins like kinases and phosphatases leading to downstream effects on diverse cellular processes. Calmodulin function extends to muscle contraction cell division and signal transduction impacting both basal and specialized cellular functions.

Pathways

Calmodulin operates as a functional link in calcium signaling and the MAPK signaling pathways. In these pathways calmodulin influences the activity of proteins such as myosin light-chain kinase and calmodulin-dependent protein kinase. These interactions showcase calmodulin's pivotal role in translating calcium signaling into cellular responses connecting several signaling cascades.

Calmodulin has associations with certain cardiovascular diseases and neurodegenerative conditions. Its relationship with ion channel dysfunction links it to disorders like long QT syndrome. Additionally calmodulin interacts with proteins like sodium-calcium exchangers or other ion-handling channels reflecting its involvement in maintaining cellular ionic balance and consequently its implication in pathological states when dysregulated.
style
left-column

Specifications

Form

Liquid

Additional notes

ab78694 is purified by using anion-exchange chromatography (DEAE sepharose resin) and gel-filtration chromatography (Sephacryl S-200) with 20mM Tris pH 7.5, 2mM EDTA.

style
left-column

General info

Function

Calmodulin acts as part of a calcium signal transduction pathway by mediating the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding (PubMed : 16760425, PubMed : 23893133, PubMed : 26969752, PubMed : 27165696, PubMed : 28890335, PubMed : 31454269, PubMed : 35568036). Calcium-binding is required for the activation of calmodulin (PubMed : 16760425, PubMed : 23893133, PubMed : 26969752, PubMed : 27165696, PubMed : 28890335, PubMed : 31454269, PubMed : 35568036). Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases, such as myosin light-chain kinases and calmodulin-dependent protein kinase type II (CaMK2), and phosphatases (PubMed : 16760425, PubMed : 23893133, PubMed : 26969752, PubMed : 27165696, PubMed : 28890335, PubMed : 31454269, PubMed : 35568036). Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed : 16760425). Is a regulator of voltage-dependent L-type calcium channels (PubMed : 31454269). Mediates calcium-dependent inactivation of CACNA1C (PubMed : 26969752). Positively regulates calcium-activated potassium channel activity of KCNN2 (PubMed : 27165696). Forms a potassium channel complex with KCNQ1 and regulates electrophysiological activity of the channel via calcium-binding (PubMed : 25441029). Acts as a sensor to modulate the endoplasmic reticulum contacts with other organelles mediated by VMP1 : ATP2A2 (PubMed : 28890335).. (Microbial infection) Required for Legionella pneumophila SidJ glutamylase activity.. (Microbial infection) Required for C.violaceum CopC and S.flexneri OspC3 arginine ADP-riboxanase activity.

Sequence similarities

Belongs to the calmodulin family.

Post-translational modifications

Ubiquitination results in a strongly decreased activity.. Phosphorylation results in a decreased activity.

Subcellular localisation

Cytoskeleton

style
left-column

Product protocols

style
left-column

Target data

Calmodulin acts as part of a calcium signal transduction pathway by mediating the control of a large number of enzymes, ion channels, aquaporins and other proteins through calcium-binding (PubMed : 16760425, PubMed : 23893133, PubMed : 26969752, PubMed : 27165696, PubMed : 28890335, PubMed : 31454269, PubMed : 35568036). Calcium-binding is required for the activation of calmodulin (PubMed : 16760425, PubMed : 23893133, PubMed : 26969752, PubMed : 27165696, PubMed : 28890335, PubMed : 31454269, PubMed : 35568036). Among the enzymes to be stimulated by the calmodulin-calcium complex are a number of protein kinases, such as myosin light-chain kinases and calmodulin-dependent protein kinase type II (CaMK2), and phosphatases (PubMed : 16760425, PubMed : 23893133, PubMed : 26969752, PubMed : 27165696, PubMed : 28890335, PubMed : 31454269, PubMed : 35568036). Together with CCP110 and centrin, is involved in a genetic pathway that regulates the centrosome cycle and progression through cytokinesis (PubMed : 16760425). Is a regulator of voltage-dependent L-type calcium channels (PubMed : 31454269). Mediates calcium-dependent inactivation of CACNA1C (PubMed : 26969752). Positively regulates calcium-activated potassium channel activity of KCNN2 (PubMed : 27165696). Forms a potassium channel complex with KCNQ1 and regulates electrophysiological activity of the channel via calcium-binding (PubMed : 25441029). Acts as a sensor to modulate the endoplasmic reticulum contacts with other organelles mediated by VMP1 : ATP2A2 (PubMed : 28890335).. (Microbial infection) Required for Legionella pneumophila SidJ glutamylase activity.. (Microbial infection) Required for C.violaceum CopC and S.flexneri OspC3 arginine ADP-riboxanase activity.
See full target information CALM1

Additional targets

,CALM3
style
left-column
style
left-column
style
right-column

Complementary Products

Secondary Antibodies

AB150113

Goat Anti-Mouse IgG H&L (Alexa Fluor® 488)

Immunocytochemistry/ Immunofluorescence - Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (AB150113)

  • 5
  • 15
Primary Antibodies

AB45689

Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal

RabMAb|Recombinant|20ul selling size

Flow Cytometry (Intracellular) - Anti-Calmodulin 1/2/3 antibody [EP799Y] - C-terminal (AB45689)

  • 4
  • 20
Proteins & Peptides

AB60867

Recombinant human HIPK1 protein

Functional Study - Recombinant human HIPK1 protein (AB60867)

  • 0
  • 0
Proteins & Peptides

AB85758

Recombinant human DCLK2 protein

Functional Studies - Recombinant human DCLK2 protein (AB85758)

  • 0
  • 0

Product promise

We are committed to supporting your work with high-quality reagents, and we're here for you every step of the way. In the unlikely event that one of our products does not perform as expected, you're protected by our Product Promise.
For full details, please see our Terms & Conditions

Please note: All products are 'FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC OR THERAPEUTIC PROCEDURES'.

For licensing inquiries, please contact partnerships@abcam.com